{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T19:44:19Z","timestamp":1769629459117,"version":"3.49.0"},"reference-count":68,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T00:00:00Z","timestamp":1632614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Greek National Funds; National Strategic Reference Framework (NSFR) 2014-2020 &quot;Support of researchers with emphasis on young researchers-cycle B&quot;, of the Operational Program &quot;Human Resource Development, Education and Lifelong Learning&quot;","award":["\u039f\u03a0\u03a3 5047896"],"award-info":[{"award-number":["\u039f\u03a0\u03a3 5047896"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Foodborne parasitic diseases represent a major threat to public health. Trichinellosis, caused by the nematode parasite Trichinella spp., is one of the most important foodborne diseases, while alariosis, caused by the trematode parasite Alaria spp., is less common in humans, and rare cases have been reported only in the USA and Canada. Both parasites can infect humans via the consumption of raw or undercooked wild boar meat. In order to investigate the prevalence of these parasites in wild boar meat in Greece, samples from the diaphragm pillars and the region of the mandibular angle from 128 wild boars, hunted in Greece, were collected. The samples were examined by classical parasitological (compression, artificial digestion, and Alaria spp. migration) and by molecular (real-time PCR) methods. For Trichinella spp. an existent real-time PCR detecting all species likely to be present in Greece was applied, while for Alaria spp. a real-time PCR was developed, employing an LNA TaqMan probe targeting the large subunit ribosomal RNA gene. All examined wild boar samples from Greece resulted negative for Trichinella and Alaria species, indicating a low prevalence of infection in the examined population. The novel real-time PCR for Alaria spp. has 81.5% amplification efficiency and is able to detect 0.12 larvae per 50 g of tissue and could be utilized as a complementary to AMT diagnostic tool in surveillance.<\/jats:p>","DOI":"10.3390\/ani11102803","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T01:59:47Z","timestamp":1632707987000},"page":"2803","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Investigation of the Food-Transmitted Parasites Trichinella spp. and Alaria spp. in Wild Boars in Greece by Classical and Molecular Methods and Development of a Novel Real-Time PCR for Alaria spp. Detection"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1580-942X","authenticated-orcid":false,"given":"Dimitris","family":"Dimzas","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3212-7458","authenticated-orcid":false,"given":"Taxiarchis","family":"Chassalevris","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 11 Stavrou Voutyra Str., 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5283-5187","authenticated-orcid":false,"given":"Zanda","family":"Ozolina","sequence":"additional","affiliation":[{"name":"Institute of Food Safety, Animal Health and Environment \u201cBIOR\u201d, Lejupes Str. 3, 1076 Riga, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2947-1765","authenticated-orcid":false,"given":"Chrysostomos I.","family":"Dovas","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 11 Stavrou Voutyra Str., 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8347-0769","authenticated-orcid":false,"given":"Anastasia","family":"Diakou","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.cofs.2021.01.006","article-title":"Burden of foodborne diseases: Think global, act local","volume":"39","author":"Pires","year":"2021","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_2","unstructured":"World Health Organization (2015). WHO Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group 2007\u20132015, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e00205","DOI":"10.1016\/j.parepi.2021.e00205","article-title":"Surveillance of foodborne parasitic diseases in Europe in a One Health approach","volume":"13","author":"Deksne","year":"2021","journal-title":"Parasite Epidemiol. Control"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/bs.afnr.2018.04.003","article-title":"Parasites in Food: From a Neglected Position to an Emerging Issue","volume":"86","author":"Robertson","year":"2018","journal-title":"Adv. Food Nutr. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.ijfoodmicro.2016.10.029","article-title":"Parasite to patient: A quantitative risk model for Trichinella spp. in pork and wild boar meat","volume":"241","author":"Franssen","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00436-009-1444-7","article-title":"Biology of Alaria spp. and human exposition risk to Alaria mesocercariae\u2014A review","volume":"105","author":"Hamedy","year":"2009","journal-title":"Parasitol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/CMR.00026-08","article-title":"Epidemiology, diagnosis, treatment, and control of trichinellosis","volume":"22","author":"Gottstein","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.rvsc.2020.08.012","article-title":"Trichinella species and genotypes","volume":"133","author":"Zarlenga","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.3201\/eid1712.110896","article-title":"Worldwide Occurrence and Impact of Human Trichinellosis, 1986\u20132009","volume":"17","author":"Murrell","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Korpysa-Dzirba, W., R\u00f3\u017cycki, M., Bilska-Zaj\u0105c, E., Karamon, J., Sroka, J., Be\u0142cik, A., Wasiak, M., and Cencek, T. (2021). Alaria alata in Terms of Risks to Consumers\u2019 Health. Foods, 10.","DOI":"10.3390\/foods10071614"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijppaw.2021.07.005","article-title":"A comparison of detection methods of Alaria alata mesocercariae in wild boar (Sus scrofa) meat","volume":"16","author":"Strokowska","year":"2021","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_12","first-page":"395","article-title":"Der \u201cDunckersche Muskelegel\u201d kann experimentell auf den Affen \u00fcbertragen werden","volume":"16","author":"Odening","year":"1961","journal-title":"Monatsh. Veterinarmed."},{"key":"ref_13","first-page":"681","article-title":"The first outbreak of trichinellosis in Greece: Study of the disease","volume":"17","author":"Livieratos","year":"1946","journal-title":"Hell. Iatriki"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Dimzas, D., Diakou, A., Koutras, C., G\u00f3mez Morales, M.A., Psalla, D., Keryttopoulos, P., Deligianni, D., Kontotasios, K., and Pozio, E. (2020). Human trichinellosis caused by Trichinella britovi in Greece, and literature review. J. Helminthol., 94.","DOI":"10.1017\/S0022149X19000075"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.vetpar.2013.10.007","article-title":"Emerging Trichinella britovi infections in free ranging pigs of Greece","volume":"199","author":"Boutsini","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e000077","DOI":"10.1136\/vetreco-2014-000077","article-title":"A serosurvey for selected pathogens in Greek European wild boar","volume":"2","author":"Touloudi","year":"2015","journal-title":"Vet. Rec. Open"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S0022149X00015960","article-title":"Helminths of foxes and other wild carnivores from rural areas in Greece","volume":"71","author":"Papadopoulos","year":"1997","journal-title":"J. Helminthol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.vetpar.2007.05.013","article-title":"Gastrointestinal parasites of shepherd and hunting dogs in the Serres Prefecture, Northern Greece","volume":"148","author":"Papazahariadou","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_19","unstructured":"Diakou, A., Karaiosif, R., Petridou, M., and Iliopoulos, Y. (2014, January 26\u201329). Endoparasites of the wolf (Canis lupus) in central Greece. Proceedings of the 11th European Wildlife Diseases Association Conference, Edinburgh, UK."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Diakou, A., Migli, D., Dimzas, D., Morelli, S., Di Cesare, A., Youlatos, D., Lymberakis, P., and Traversa, D. (2021). Endoparasites of european wildcats (Felis silvestris) in Greece. Pathogens, 10.","DOI":"10.3390\/pathogens10050594"},{"key":"#cr-split#-ref_21.1","unstructured":"European-Commission (2015). Commission regulation"},{"key":"#cr-split#-ref_21.2","unstructured":"(EC) no 2015\/1375 of 10 August 2015 laying down specific rules for Trichinella in meat. Off. J. Eur. Union, 212, 7-34."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/S0020-7519(99)00107-1","article-title":"A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella","volume":"29","author":"Zarlenga","year":"1999","journal-title":"Int. J. Parasitol."},{"key":"ref_23","first-page":"299","article-title":"PCR-derived methods for the identification of Trichinella parasites from animal and human samples","volume":"216","author":"Pozio","year":"2003","journal-title":"Methods Mol. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.vetpar.2012.03.054","article-title":"Real-time PCR as a surveillance tool for the detection of Trichinella infection in muscle samples from wildlife","volume":"188","author":"Cuttell","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1007\/s00436-010-2240-0","article-title":"Development of a PCR approach for differentiation of Alaria spp. mesocercariae","volume":"108","author":"Riehn","year":"2011","journal-title":"Parasitol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ijppaw.2020.04.006","article-title":"The occurrence of Alaria alata mesocercariae in wild boars (Sus scrofa) in north-eastern Poland","volume":"12","author":"Strokowska","year":"2020","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_27","unstructured":"Dupouy-Camet, J., and Murrell, K.D. (2007). Detection and surveillance for Trichinella: Meat inspection and hygiene, and legislation. FAO\/WHO\/OIE Guidelines for the Surveillance, Management, Prevention and Control of Trichinellosis, World Organization for Animal Health (OIE)."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00436-010-1853-7","article-title":"A novel detection method for Alaria alata mesocercariae in meat","volume":"107","author":"Riehn","year":"2010","journal-title":"Parasitol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.4315\/0362-028X-66.6.1043","article-title":"Comparison of a modified digestion assay with trichinoscopy for the detection of Trichinella larvae in pork","volume":"66","author":"Forbes","year":"2003","journal-title":"J. Food Prot."},{"key":"ref_30","first-page":"55354","article-title":"Magnetic stirrer method for the detection of Trichinella larvae in muscle samples","volume":"2017","author":"Pozio","year":"2017","journal-title":"J. Vis. Exp."},{"key":"ref_31","unstructured":"Dupouy-Camet, J., and Murrell, K.D. (2007). FAO\/WHO\/OIE Guidelines for the Surveillance, Management, Prevention and Control of Trichinellosis, World Organisation for Animal Health (OIE)."},{"key":"ref_32","unstructured":"Ministry of Agriculture Fisheries and Food (MAFF) (1986). Manual of Veterinary Parasitological Techniques."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"101528","DOI":"10.1016\/j.mcp.2020.101528","article-title":"A highly sensitive semi-nested real-time PCR utilizing oligospermine-conjugated degenerate primers for the detection of diverse strains of small ruminant lentiviruses","volume":"51","author":"Chassalevris","year":"2020","journal-title":"Mol. Cell. Probes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"W163","DOI":"10.1093\/nar\/gkn198","article-title":"IDT SciTools: A suite for analysis and design of nucleic acid oligomers","volume":"36","author":"Owczarzy","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9352","DOI":"10.1021\/bi200904e","article-title":"Stability and mismatch discrimination of locked nucleic acid-DNA duplexes","volume":"50","author":"Owczarzy","year":"2011","journal-title":"Biochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e60","DOI":"10.1093\/nar\/gkl175","article-title":"Design of LNA probes that improve mismatch discrimination","volume":"34","author":"You","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_39","unstructured":"FAO (2021). Parasites in Foods: An Invisible Threat, Food and Agriculture Organization of the United Nations. Food Safety Technical Toolkit for Asia and the Pacific No. 7."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3892\/etm.2021.10327","article-title":"Trichinellosis in hospitalized patients from Western Romania: A retrospective study","volume":"22","author":"Lupu","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"EFSA (European Food Safety Authority), and ECDC (European Centre for Disease Prevention and Control) (2018). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J., 16, e05500.","DOI":"10.2903\/j.efsa.2018.5500"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"EFSA (European Food Safety Authority), and ECDC (European Centre for Disease Prevention and Control) (2021). The European Union One Health 2019 Zoonoses Report. EFSA J., 19, e06406.","DOI":"10.2903\/j.efsa.2021.6406"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.pt.2013.09.005","article-title":"Impacts of globalisation on foodborne parasites","volume":"30","author":"Robertson","year":"2014","journal-title":"Trends Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"803","DOI":"10.4269\/ajtmh.1976.25.803","article-title":"Fatal human infection with mesocercariae of the trematode Alaria americana","volume":"25","author":"Freeman","year":"1976","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_45","first-page":"1111","article-title":"Systemic infection with Alaria americana (Trematoda)","volume":"115","author":"Fernandes","year":"1976","journal-title":"Can. Med. Assoc. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"422","DOI":"10.4269\/ajtmh.1977.26.422","article-title":"Mesocercaria in the skin of man in Louisiana","volume":"26","author":"Beaver","year":"1977","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0002-9394(14)70003-0","article-title":"Two cases of intraocular infection with Alaria mesocercaria (Trematoda)","volume":"117","author":"McDonald","year":"1994","journal-title":"Am. J. Ophthalmol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/zph.12800","article-title":"Trichinella britovi infection in wild boar in Portugal","volume":"68","author":"Fernandes","year":"2021","journal-title":"Zoonoses Public Health"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"100172","DOI":"10.1016\/j.onehlt.2020.100172","article-title":"Trichinella spp. in wild boar (Sus scrofa) populations in Croatia during an eight-year study (2010\u20132017)","volume":"11","author":"Marucci","year":"2020","journal-title":"One Health"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"361","DOI":"10.26444\/aaem\/125194","article-title":"Trichinellosis in Slovakia\u2013epidemiological situation in humans and animals (2009\u20132018)","volume":"27","year":"2020","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13028-020-00539-x","article-title":"Surveillance of important bacterial and parasitic infections in Danish wild boars (Sus scrofa)","volume":"62","author":"Petersen","year":"2020","journal-title":"Acta Vet. Scand."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1515\/jvetres-2017-0023","article-title":"Epizootic and epidemiological situation of Trichinella sp. infection in Poland in 2006\u20132015 in view of wild boar population dynamics","volume":"61","author":"Flis","year":"2017","journal-title":"J. Vet. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1007\/s00436-018-5908-5","article-title":"Occurrence of selected zoonotic food-borne parasites and first molecular identification of Alaria alata in wild boars (Sus scrofa) in Italy","volume":"117","author":"Gazzonis","year":"2018","journal-title":"Parasitol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.fm.2016.12.012","article-title":"Meat sources of infection for outbreaks of human trichinellosis","volume":"64","author":"Rostami","year":"2017","journal-title":"Food Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-020-04394-7","article-title":"Differences in larval survival and IgG response patterns in long-lasting infections by Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis in pigs","volume":"13","author":"Pozio","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1186\/1756-3305-7-171","article-title":"Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa)","volume":"7","author":"Ludovisi","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetpar.2013.09.029","article-title":"Increasing circulation of Alaria alata mesocercaria in wild boar populations of the Rhine valley, France, 2007\u20132011","volume":"199","author":"Portier","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_58","first-page":"100485","article-title":"The wild boar (Sus scrofa, Linnaeus, 1758) as an important reservoir host for Alaria alata in the Baltic region and potential risk of infection in humans","volume":"22","author":"Mateusa","year":"2020","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_59","first-page":"25","article-title":"Effectiveness of two methods for mesocercariae of Alaria alata detection in wild boars (Sus scrofa)","volume":"15","author":"Deksne","year":"2017","journal-title":"Environ. Exp. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1007\/s00436-012-2936-4","article-title":"Alaria alata in wild boars (Sus scrofa, linnaeus, 1758) in the eastern parts of Germany","volume":"111","author":"Riehn","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_61","first-page":"345","article-title":"Alaria alata mesocercariae in wild boars (Sus scrofa) in northern Serbia after the flood disaster of 2014","volume":"103","author":"Malesevic","year":"2016","journal-title":"Wien. Tierarztl. Monatsschr."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.vetpar.2013.05.024","article-title":"Alaria alata mesocercariae in wild boar (Sus scrofa, Linnaeus, 1758) in south regions of the Czech Republic","volume":"197","author":"Paulsen","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s10344-012-0642-2","article-title":"Findings of Alaria alata mesocercariae in wild boars (Sus scrofa) in eastern Austria","volume":"58","author":"Paulsen","year":"2012","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00436-020-06914-x","article-title":"Occurrence of Alaria alata in wild boars (Sus scrofa) in Poland and detection of genetic variability between isolates","volume":"120","author":"Marucci","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_65","first-page":"120","article-title":"Findings of Alaria alata mesocercariae in wild boars (Sus scrofa, Linnaeus, 1758) in west Hungary (Transdanubia regions)","volume":"101","author":"Berger","year":"2014","journal-title":"Wien. Tierarztl. Monatsschr."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1007\/s00436-021-07178-9","article-title":"Prevalence of Alaria alata mesocercariae in wild boars from Brandenburg, Germany","volume":"120","author":"Bier","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"K\u00e4stner, C., Bahn, P., Sch\u00f6nfelder, R., Ozoli\u0146a, Z., Alksne, L., Richter, M.H., Deksne, G., Mayer-Scholl, A., and Johne, A. (2021). Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry. Microorganisms, 9.","DOI":"10.3390\/microorganisms9081664"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2803\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:05:18Z","timestamp":1760166318000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,26]]},"references-count":68,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ani11102803"],"URL":"https:\/\/doi.org\/10.3390\/ani11102803","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,26]]}}}