{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:21:44Z","timestamp":1772893304561,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K09794"],"award-info":[{"award-number":["19K09794"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K07117"],"award-info":[{"award-number":["19K07117"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Smoking Research Foundation of Japan","award":["2020G006"],"award-info":[{"award-number":["2020G006"]}]},{"name":"Kobayashi Foundation","award":["181"],"award-info":[{"award-number":["181"]}]},{"name":"Kobayashi Foundation","award":["215"],"award-info":[{"award-number":["215"]}]},{"name":"The Grant of National Center for Child Health and Development","award":["2021-A1"],"award-info":[{"award-number":["2021-A1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>17\u03b2-hydroxysteroid dehydrogenase type 3 (HSD17B3) converts androstenedione (A4) into testosterone (T), which regulates sex steroid production. Because various mutations of the HSD17B3 gene cause disorder of sex differentiation (DSD) in multiple mammalian species, it is very important to reveal the molecular characteristics of this gene in various species. Here, we revealed the open reading frame of the ovine HSD17B3 gene. Enzymatic activities of ovine HSD17B3 and HSD17B1 for converting A4 to T were detected using ovine androgen receptor-mediated transactivation in reporter assays. Although HSD17B3 also converted estrone to estradiol, this activity was much weaker than those of HSD17B1. Although ovine HSD17B3 has an amino acid sequence that is conserved compared with other mammalian species, it possesses two amino acid substitutions that are consistent with the reported variants of human HSD17B3. Substitutions of these amino acids in ovine HSD17B3 for those in human did not affect the enzymatic activities. However, enzymatic activities declined upon missense mutations of the HSD17B3 gene associated with 46,XY DSD, affecting amino acids that are conserved between these two species. The present study provides basic information and tools to investigate the molecular mechanisms behind DSD not only in ovine, but also in various mammalian species.<\/jats:p>","DOI":"10.3390\/ani11102876","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T10:55:40Z","timestamp":1633085740000},"page":"2876","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Analyses of Molecular Characteristics and Enzymatic Activities of Ovine HSD17B3"],"prefix":"10.3390","volume":"11","author":[{"given":"Mohammad Sayful","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Asahikawa Medical University, Asahikawa 078-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6123-9061","authenticated-orcid":false,"given":"Junsuke","family":"Uwada","sequence":"additional","affiliation":[{"name":"Department of 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National Research Institute for Child Health and Development, Tokyo 157-8535, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0283-9910","authenticated-orcid":false,"given":"Nobuo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 927-0553, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Sekiguchi","sequence":"additional","affiliation":[{"name":"Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 927-0553, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4526-6585","authenticated-orcid":false,"given":"Takashi","family":"Yazawa","sequence":"additional","affiliation":[{"name":"Department of 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