{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:50:42Z","timestamp":1788173442601,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Israeli Cattle Division of the Ministry of Agriculture","award":["820-0339-17"],"award-info":[{"award-number":["820-0339-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>An association between progressive motility (PM) and spermatozoa fertility competence has been suggested. However, the mechanism that underlies PM is not clear enough. We examined physiological characteristics and fatty acid composition of fresh spermatozoa with high and low PM. Additional analysis of fatty acid composition and structural characteristics was performed on spermatozoa samples with high and low progressively motile spermatozoa\u2019s survival (PMSS), i.e., the ratio between the proportion of progressively motile spermatozoa after and before cryopreservation. Finally, a fertility field trial was conducted to examine the association between the number of PM spermatozoa within the insemination straw post thawing and conception rate. Analysis of fresh spermatozoa revealed a higher omega-6 to omega-3 ratio in ejaculates with low PM relative to those with high PM (p &lt; 0.01). The proportion of polyunsaturated fatty acids was higher in low-PMSS fresh samples (p &lt; 0.05) relative to their high-PMSS counterparts. Fresh samples with high-PMSS expressed a higher mitochondrial membrane potential (p &lt; 0.05) and a higher proportion of viable cells that expressed reactive oxygen species (ROS; p &lt; 0.05). Post-thawing evaluation revealed a reduced proportion of progressively motile sperm, with a prominent effect in samples with high PM relative to low PM, defined before freezing (p &lt; 0.01). No differences in spermatozoa mitochondrial membrane potential or ROS level were found post-thawing. A fertility study revealed a positive correlation between the number of progressively motile spermatozoa within a standard insemination straw and conception rate (p &lt; 0.05). Considering these, the bull PMSS is suggested to be taken into account at the time of straw preparation.<\/jats:p>","DOI":"10.3390\/ani11102948","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T09:33:02Z","timestamp":1634117582000},"page":"2948","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Association between Fatty Acid Composition, Cryotolerance and Fertility Competence of Progressively Motile Bovine Spermatozoa"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-9702","authenticated-orcid":false,"given":"Tanya","family":"Kogan","sequence":"first","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"},{"name":"Sion Artificial Insemination Center, Gedera 7057102, Israel"},{"name":"Animal Sperm Research Center (ASRC), Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Grossman Dahan","sequence":"additional","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronit","family":"Laor","sequence":"additional","affiliation":[{"name":"Sion Artificial Insemination Center, Gedera 7057102, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurit","family":"Argov-Argaman","sequence":"additional","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoel","family":"Zeron","sequence":"additional","affiliation":[{"name":"Sion Artificial Insemination Center, Gedera 7057102, Israel"},{"name":"Animal Sperm Research Center (ASRC), Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9610-3674","authenticated-orcid":false,"given":"Alisa","family":"Komsky-Elbaz","sequence":"additional","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"},{"name":"Animal Sperm Research Center (ASRC), Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorit","family":"Kalo","sequence":"additional","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"},{"name":"Animal Sperm Research Center (ASRC), Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zvi","family":"Roth","sequence":"additional","affiliation":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"},{"name":"Animal Sperm Research Center (ASRC), Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2016.04.013","article-title":"Cryopreservation of bull semen: Evolution from egg yolk based to soybean based extenders","volume":"172","author":"Layek","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13028-018-0396-2","article-title":"Sperm quality in frozen beef and dairy bull semen","volume":"60","author":"Morrell","year":"2018","journal-title":"Acta Vet. Scand."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1439-0531.2010.01603.x","article-title":"Objective Sperm Motion Analysis to Assess Dairy Bull Fertility Using Computer-Aided System\u2014A Review","volume":"46","author":"Kathiravan","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/j.1365-2605.2007.00799.x","article-title":"Prediction of spontaneous conception based on semen parameters","volume":"31","author":"Jedrzejczak","year":"2008","journal-title":"Int. J. Androl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3109\/19396368.2011.553984","article-title":"Sperm DNA damage or progressive motility: Which one is the better predictor of fertilization in vitro?","volume":"57","author":"Simon","year":"2011","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Brinsko, S.P., Blanchard, T.L., Varner, D.D., Schumacher, J., Love, C.C., Hinrichs, K., and Hartman, D.L. (2011). Examination of the Stallion for Breeding Soundness. Manual of Equine Reproduction, Elsevier. [3rd ed.].","DOI":"10.1016\/B978-0-323-06482-8.00022-3"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/j.theriogenology.2016.05.010","article-title":"Semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season","volume":"86","author":"Ahmed","year":"2016","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.anireprosci.2007.01.002","article-title":"Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes","volume":"104","author":"Kathiravan","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1017\/S0967199414000720","article-title":"Progressive motility\u2014A potential predictive parameter for semen fertilization capacity in bovines","volume":"24","author":"Li","year":"2016","journal-title":"Zygote"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Agarwal, A., Gupta, S., Hamada, A., and Wasik, M. (2012). Sperm Banking: Indications and Regulations. Infertility: Diagnosis, management and IVF, Jaypee Brothers Medical Publishers Ltd.","DOI":"10.5005\/jp\/books\/11639_29"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s40104-016-0076-x","article-title":"Sperm cryodamage occurs after rapid freezing phase: Flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation","volume":"7","author":"Castro","year":"2016","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_12","first-page":"173","article-title":"Effect of Cryopreservation on Casa Characteristics, Mitochondrial Transmembrane Potential, Plasma and Acrosome Integrities, Morphology and in vivo Fertility of Buffalo Bull Spermatozoa","volume":"40","author":"Ahmed","year":"2019","journal-title":"Cryo Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation of Mammalian Sperm: What We Ask Them to Survive","volume":"11","author":"Hammerstedt","year":"1990","journal-title":"J. Androl."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Minireview: Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon","volume":"21","author":"Bailey","year":"2000","journal-title":"J. Androl."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.theriogenology.2010.10.032","article-title":"Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility","volume":"75","author":"Kirkwood","year":"2011","journal-title":"Theriogenology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/rda.12860","article-title":"Lipid composition of the canine sperm plasma membrane as markers of sperm motility","volume":"52","author":"Lucio","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1530\/REP-12-0498","article-title":"Season-induced variation in lipid composition is associated with semen quality in Holstein bulls","volume":"145","author":"Mahgrefthe","year":"2013","journal-title":"Reproduction"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.theriogenology.2008.05.023","article-title":"Performance evaluation of the SQA-Vb automated sperm quality analyzer for bulls","volume":"70","author":"Shalit","year":"2008","journal-title":"Theriogenology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.theriogenology.2013.05.024","article-title":"Variation in lipid profiles within semen compartments-the bovine model of aging","volume":"80","author":"Mahgrefthe","year":"2013","journal-title":"Theriogenology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.theriogenology.2015.07.035","article-title":"Use of combinations of in vitro quality assessments to predict fertility of bovine semen","volume":"84","author":"Sellem","year":"2015","journal-title":"Theriogenology"},{"key":"ref_21","first-page":"e58622","article-title":"Fluorimetric techniques for the assessment of sperm membranes","volume":"2018","author":"Roth","year":"2018","journal-title":"J. Vis. Exp."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Casares, D., Escrib\u00e1, P.V., and Rossell\u00f3, C.A. (2019). Membrane lipid composition: Effect on membrane and organelle structure, function and compartmentalization and therapeutic avenues. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20092167"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1093\/molehr\/4.2.111","article-title":"The fatty acid composition of phospholipids of spermatozoa from infertile patients","volume":"4","author":"Zalata","year":"1998","journal-title":"Mol. Hum. Reprod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.clnu.2009.07.008","article-title":"Relationship of omega-3 and omega-6 fatty acids with semen characteristics, and anti-oxidant status of seminal plasma: A comparison between fertile and infertile men","volume":"29","author":"Safarinejad","year":"2010","journal-title":"Clin. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1017\/S0033583500001645","article-title":"Physical principles of membrane organization","volume":"13","author":"Israelachvili","year":"1980","journal-title":"Q. Rev. Biophys."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1016\/S0022-2275(20)32521-9","article-title":"Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey sperm","volume":"39","author":"Connor","year":"1998","journal-title":"J. Lipid Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1439-0272.1983.tb00374.x","article-title":"Polyunsaturated Fatty Acids in Relation to Sperm Motility","volume":"15","author":"Nissen","year":"1983","journal-title":"Andrologia"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.theriogenology.2017.01.011","article-title":"Cryopreservation of bull semen is associated with carbonylation of sperm proteins","volume":"92","author":"Mostek","year":"2017","journal-title":"Theriogenology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1002\/mrd.22871","article-title":"Reactive oxygen species as mediators of sperm capacitation and pathological damage","volume":"84","author":"Aitken","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0015-0282(16)59520-X","article-title":"The ultrastructural pathology of the spermatozoon as a cause of infertility: The role of electron microscopy in the evaluation of semen quality","volume":"48","author":"Wallach","year":"1987","journal-title":"Fertil. Steril."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1093\/humrep\/17.3.704","article-title":"The effects of cryopreservation on sperm morphology, motility and mitochondrial function","volume":"17","author":"McClure","year":"2002","journal-title":"Hum. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1093\/humrep\/der281","article-title":"Sperm storage for cancer patients in the UK: A review of current practice","volume":"26","author":"Sharma","year":"2011","journal-title":"Hum. Reprod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1016\/j.freeradbiomed.2003.08.010","article-title":"In situ detection and localization of lipid peroxidation in individual bovine sperm cells","volume":"35","author":"Brouwers","year":"2003","journal-title":"Free Radic. Biol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.anireprosci.2007.02.001","article-title":"Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin","volume":"104","author":"Celeghini","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vincent, P., Underwood, S.L., Dolbec, C., Bouchard, N., Kroetsch, T., and Blondin, P. (2014). Bovine Semen Quality Control in Artificial Insemination Centers. Bovine Reproduction, John Wiley & Sons, Inc.","DOI":"10.1002\/9781118833971.ch74"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.theriogenology.2020.07.005","article-title":"Differences in sperm functionality and intracellular metabolites in Norwegian Red bulls of contrasting fertility","volume":"157","author":"Narud","year":"2020","journal-title":"Theriogenology"},{"key":"ref_37","first-page":"39","article-title":"In vitro evaluation of sperm quality related to in vivo function and fertility","volume":"64","author":"Barth","year":"2007","journal-title":"Soc. Reprod. Fertil. Suppl."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2948\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:12:24Z","timestamp":1760166744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,13]]},"references-count":37,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ani11102948"],"URL":"https:\/\/doi.org\/10.3390\/ani11102948","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,13]]}}}