{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:36:39Z","timestamp":1780104999172,"version":"3.54.0"},"reference-count":60,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Li Chen","award":["2016NYZ0046"],"award-info":[{"award-number":["2016NYZ0046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>China officially promulgated the announcement of banning the use of antibiotics in the animal industry in 2020. However, the prohibition of antibiotics in the animal industry would reduce the feed conversion rate and increase the mortality of animals. In order to obtain information about the pathogenesis and host immune response of rabbits with diarrhea after being fed an antibiotic-free diet, we first analyzed the intestinal tissue sections of rabbits. Secondly, the gene expression differences of rabbit intestinal segments were analyzed by high-throughput sequencing. Our analysis identified 168, 593, 2069, 334, 321, and 1423 DEGs in the comparison groups S_Z (the duodenum of healthy rabbits) vs. S_B (diarrhea in the duodenum of rabbits), K_Z (healthy rabbit jejunum) vs. K_B (rabbits with diarrhea in the jejunum), H_Z (healthy rabbit ileum) vs. H_B (rabbits with diarrhea in the ileum), M_Z (healthy cecum of rabbits) vs. M_B (rabbits with diarrhea in the cecum), J_Z (healthy rabbit colon) vs. J_B (colon of rabbits with diarrhea), and Z_Z (healthy rabbit rectum) vs. Z_B (rectum of rabbits with diarrhea), respectively. The reproducibility and repeatability of the results were validated by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed the host DEGs that are potentially related to acute inflammation, stress response, tissue dehydration, adaptive immune response, protein binding, activation of related enzymes, migration of immune cells, and so on. In this descriptive study, our findings revealed the changes in the host transcriptome expression profile after feeding an antibiotic-free diet and suggested that feeding an antibiotic-free diet alters the host\u2019s metabolic network and the expression of antiviral proteins, which provides a theoretical basis for further study on the immune response of animals fed an antibiotic-free diet.<\/jats:p>","DOI":"10.3390\/ani11102994","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T01:23:46Z","timestamp":1634693026000},"page":"2994","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptomics Analysis Reveals the Immune Response Mechanism of Rabbits with Diarrhea Fed an Antibiotic-Free Diet"],"prefix":"10.3390","volume":"11","author":[{"given":"Li","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Du","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimei","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1399-7079","authenticated-orcid":false,"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal  Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6885-4104","authenticated-orcid":false,"given":"Xianbo","family":"Jia","sequence":"additional","affiliation":[{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal  Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songjia","family":"Lai","sequence":"additional","affiliation":[{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal  Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,19]]},"reference":[{"key":"ref_1","unstructured":"Rosen, G.D. (2020, March 10). Antibacterials in Poultry And Pig Nutrition. City: Biotechnology in Animal Feeds and Animal Feeding. Available online: https:\/\/xueshu.baidu.com\/usercenter\/paper\/show?paperid=e831e3a2036244c8207aa42278c3e699&site=xueshu_se."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e27949","DOI":"10.1371\/journal.pone.0027949","article-title":"Modulations of the Chicken Cecal Microbiome and Metagenome in Response to Anticoccidial and Growth Promoter Treatment","volume":"6","author":"Danzeisen","year":"2011","journal-title":"PLoS ONE"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Low, C.X., Tan, T.H., Mutalib, N.S.A., Pusparajah, P., and Lee, L.H. (2021). Unveiling the Impact of Antibiotics and Alternative Methods for Animal Husbandry: A Review. Antibiotics, 10.","DOI":"10.3390\/antibiotics10050578"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1056\/NEJM200110183451610","article-title":"Antimicrobial use in animal feed\u2014Time to stop","volume":"345","author":"Gorbach","year":"2001","journal-title":"N. Engl. J. Med."},{"key":"ref_5","unstructured":"Kjeld, P.V. (2021, October 08). Alliance for the Prudent Use of Antibiotics, Available online: https:\/\/www.bookdepository.com\/Alliance-for-Prudent-Use-Antibiotics-Pollux-Variste-Kjeld\/9786135810288."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1093\/jac\/dkg313","article-title":"The European ban on growth-promoting antibiotics and emerging consequences for human and animal health","volume":"52","author":"Mark","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Callaway, T.R., Lillehoj, H., Chuanchuen, R., and Gay, C.G. (2021). Alternatives to Antibiotics: A Symposium on the Challenges and Solutions for Animal Health and Production. Antibiotics, 10.","DOI":"10.3390\/antibiotics10050471"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1155\/1999\/485237","article-title":"Helicobacter pylori: Novel Therapies","volume":"13","author":"Drouin","year":"1999","journal-title":"Can. J. Gastroenterol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0167-5877(99)00002-1","article-title":"Risk factors for Salmonella enterica subsp. enterica contamination in French broiler-chicken flocks at the end of the rearing period","volume":"39","author":"Rosea","year":"1999","journal-title":"Prev. Vet. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/03079450020023230","article-title":"Severely impaired production performance in broiler flocks with high incidence of Clostridium perfringens-associated hepatitis","volume":"30","author":"Lovland","year":"2001","journal-title":"Avian Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1046\/j.1462-2920.2003.00461.x","article-title":"The hidden lifestyles of Bacillus cereus and relatives","volume":"5","author":"Jensen","year":"2003","journal-title":"Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev.cellbio.042308.113344","article-title":"Vertebrate Endoderm Development and Organ Formation","volume":"1","author":"Zorn","year":"2009","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nri3608","article-title":"Intestinal epithelial cells: Regulators of barrier function and immune homeostasis","volume":"14","author":"Peterson","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature11552","article-title":"Functional interactions between the gut microbiota and host metabolism","volume":"489","author":"Tremaroli","year":"2012","journal-title":"Nature"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1038\/nature05484","article-title":"Gut hormones and the regulation of energy homeostasis","volume":"444","author":"Murphy","year":"2007","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/nrd2444","article-title":"Hormones of the gut-brain axis as targets for the treatment of upper GI disorders","volume":"7","author":"Sanger","year":"2008","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_17","first-page":"494","article-title":"Intestinal flora, intestinal mucosal immunity and the related intestinal diseases: Research progress","volume":"29","author":"Wang","year":"2017","journal-title":"Chin. J. Microecol."},{"key":"ref_18","first-page":"797","article-title":"DANMAP 2012: Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark","volume":"9","author":"Andersen","year":"2012","journal-title":"J. Veg. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.5424\/sjar\/200806S1-5346","article-title":"New trends in rabbit feeding: Influence of nutrition on intestinal health. A review","volume":"6","author":"Carabano","year":"2008","journal-title":"Span. J. Agric. Res."},{"key":"ref_20","first-page":"243","article-title":"Histological and ultrastructural findings in commercial bred rabbits exhibiting severe diarrhea","volume":"32","author":"Beltz","year":"2005","journal-title":"Scand. J. Lab. Anim. Ence"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1053\/saep.2001.19794","article-title":"Normal bacterial flora of the rabbit gastrointestinal tract: A clinical approach","volume":"10","author":"Fann","year":"2001","journal-title":"Semin. Avian Exot. Pet Med."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tang, T., Li, Y., Wang, J., Elzo, M.A., and Lai, S. (2021). Untargeted Metabolomics Reveals Intestinal Pathogenesis and Self-Repair in Rabbits Fed an Antibiotic-Free Diet. Animals, 11.","DOI":"10.3390\/ani11061560"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e123","DOI":"10.1093\/nar\/gkp596","article-title":"Transcriptome analysis by strand-specific sequencing of complementary DNA","volume":"37","author":"Parkhomchuk","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_26","first-page":"621","article-title":"Mapping and quantifying mammalian transcriptomes by RNA-Seq","volume":"5","author":"Mortazavi","year":"2008","journal-title":"Nature"},{"key":"ref_27","unstructured":"Gao, T., Chongyang, H., Jiaye, C., Chen, B., Shi, Y., and Zhi, F. (2021). An IRF1-dependent pathway of TNF\u03b1-induced shedding in intestinal epithelial cells. J. Crohns Colitis, jjab134."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S314","DOI":"10.1093\/clinids\/13.Supplement_4.S314","article-title":"Morphology of rectal mucosa of patients with shigellosis","volume":"13","author":"Mathan","year":"1991","journal-title":"Rev. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/0016-5085(86)91120-0","article-title":"Rectal histology in acute bacillary dysentery","volume":"90","author":"Anand","year":"1986","journal-title":"Gastroenterology"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Crowley, S.M., Allaire, J.M., Han, X., Graef, F.A., Stahl, M., Knodler, L., and Vallance, B. (2020). A46 the inflammatory caspases coordinate mucosal restriction of salmonella through the epithelial-intrinsic inflammasome and il-22 driven mucin secretion. J. Can. Assoc. Gastroenterol., 54\u201355.","DOI":"10.1093\/jcag\/gwz047.045"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0968-0004(01)02007-2","article-title":"Evolution of enzyme cascades from embryonic development to blood coagulation","volume":"27","author":"Krem","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1078\/0171-2985-00137","article-title":"Which came first, the lectin\/classical pathway or the alternative pathway of complement?","volume":"205","author":"Dodds","year":"2002","journal-title":"Immunobiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0952-7915(01)00302-8","article-title":"The molecular basis of innate immunity in the horseshoe crab","volume":"14","author":"Iwanaga","year":"2002","journal-title":"Curr. Opin. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/ni.1923","article-title":"Complement: A key system for immune surveillance and homeostasis","volume":"11","author":"Ricklin","year":"2010","journal-title":"Nat. Immunol"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8720","DOI":"10.1073\/pnas.94.16.8720","article-title":"Abrogation of the alternative complement pathway by targeted deletion of murine factor B","volume":"94","author":"Matsumoto","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Williams, J.A., Stampoulis, D., Gunter, C.E., John, G., Peter, A., Moss, S.E., and Thomas, L. (2016). Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0161898"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1111\/j.1462-5822.2007.00981.x","article-title":"Macrophage complement receptors and pathogen clearance","volume":"9","author":"Campagne","year":"2007","journal-title":"Cell. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.imbio.2006.11.005","article-title":"The clearance of apoptotic cells by complement","volume":"212","author":"Flierman","year":"2007","journal-title":"Immunobiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.molimm.2007.09.008","article-title":"Role of complement and complement regulators in the removal of apoptotic cells","volume":"45","author":"Trouw","year":"2008","journal-title":"Mol. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jprot.2014.03.019","article-title":"Effect of dietary fiber\/starch balance on the cecal proteome of growing rabbits","volume":"103","author":"Zhu","year":"2014","journal-title":"J. Proteom."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1096\/fj.08-110221","article-title":"Hypoxia inducible factor-1 (HIF-1)-mediated repression of cystic fibrosis transmembrane conductance regulator (CFTR) in the intestinal epithelium","volume":"23","author":"Zheng","year":"2009","journal-title":"FASEB J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/nrg2665","article-title":"Generating specificity and diversity in the transcriptional response to hypoxia","volume":"10","author":"Lendahl","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1177\/0148607195019006502","article-title":"Glutamine dipeptide-supplemented parenteral nutrition reverses gut atrophy, disaccharidase enzyme activity, and absorption in rats","volume":"19","author":"Wardelmann","year":"1995","journal-title":"JPEN J. Parenter Enter. Nutr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5239","DOI":"10.3892\/mmr.2015.4079","article-title":"Imbalance of tumor necrosis factor-\u03b1, interleukin-8 and interleukin-10 production evokes barrier dysfunction, severe abdominal symptoms and psychological disorders in patients with irritable bowel syndrome-associated diarrhea","volume":"12","author":"Zhen","year":"2015","journal-title":"Mol. Med. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1146\/annurev.immunol.021908.132710","article-title":"IL-17 and Th17 Cells","volume":"8","author":"Korn","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.cyto.2007.11.013","article-title":"Structure-function relationships in the IL-17 receptor: Implications for signal transduction and therapy","volume":"41","author":"Shen","year":"2008","journal-title":"Cytokine"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/j.immuni.2008.07.018","article-title":"Interleukin-17 promotes autoimmunity by triggering a positive-feedback loop via interleukin-6 induction","volume":"29","author":"Ogura","year":"2008","journal-title":"Immunity"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1365-2567.2009.03240.x","article-title":"Interleukin-17 and its target genes: Mechanisms of interleukin-17 function in disease","volume":"129","author":"Onishi","year":"2010","journal-title":"Immunology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1038\/ni1412","article-title":"Nod-like proteins in immunity, inflammation and disease","volume":"7","author":"Fritz","year":"2006","journal-title":"Nat. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, X.Q., Zhu, Y.H., Zhang, H.F., Yue, Y., Cai, Z.X., Lu, Q.P., Zhang, L., Weng, X.G., Zhang, F.J., and Zhou, D. (2012). Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea: Intestinal microbiota and immune imbalances. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040666"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1002\/path.2287","article-title":"TNF-mediated inflammatory disease","volume":"214","author":"Bradley","year":"2008","journal-title":"J. Pathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1093\/rheumatology\/keq031","article-title":"Transmembrane TNF-alpha: Structure, function and interaction with anti-TNF agents","volume":"49","author":"Horiuchi","year":"2010","journal-title":"Rheumatology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/acr.20371","article-title":"Systematic review and meta-analysis: Anti-tumor necrosis factor \u03b1 therapy and cardiovascular events in rheumatoid arthritis","volume":"63","author":"Barnabe","year":"2011","journal-title":"Arthritis Care Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1126\/science.285.5425.248","article-title":"HMG-1 as a late mediator of endotoxin lethality in mice","volume":"285","author":"Wang","year":"1999","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1080\/21505594.2018.1536632","article-title":"Porcine transmissible gastroenteritis virus nonstructural protein 2 contributes to inflammation via NF-\u03baB activation","volume":"9","author":"Wang","year":"2018","journal-title":"Virulence"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2174\/187221310791163071","article-title":"Signaling pathways associated with inflammatory bowel disease","volume":"4","author":"Wei","year":"2010","journal-title":"Recent Pat. Inflamm. Allergy Drug Discov."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1042\/BST0340647","article-title":"Signalling through Class I PI3Ks in mammalian cells","volume":"34","author":"Hawkins","year":"2006","journal-title":"Biochem. Soc. Trans."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1042\/bst0320480","article-title":"Regulation of granulocyte apoptosis by phosphatidylinositol 3-kinase","volume":"32","author":"Lindemans","year":"2004","journal-title":"Biochem. Soc. Trans."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0898-6568(02)00063-3","article-title":"Involvement of phosphatidylinositol 3-kinase gamma in neutrophil apoptosis","volume":"15","author":"Yang","year":"2003","journal-title":"Cell Signal."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1002\/eji.200425091","article-title":"The survival effect of TNF-alpha in human neutrophils is mediated via NF-kappa B-dependent IL-8 release","volume":"34","author":"Cowburn","year":"2004","journal-title":"Eur. J. Immunol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2994\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:17:39Z","timestamp":1760167059000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,19]]},"references-count":60,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ani11102994"],"URL":"https:\/\/doi.org\/10.3390\/ani11102994","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,19]]}}}