{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T07:13:46Z","timestamp":1774595626001,"version":"3.50.1"},"reference-count":23,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. The aim of this study was to perform the analytical validation of a new immunoenzymatic method for the measurement of feline PTH. Thirty-eight cats affected with chronic kidney disease (CKD) were included. PTH was measured using a two-site immunoenzymatic method validated in humans and dogs (ST AIA-PACK\u00ae Intact PTH, Tosoh Bioscience, Tessenderlo, Belgium). The analytical validation provided the evaluation of precision (intra-assay and inter-assay), accuracy (linearity under dilution (LUD) and spike recovery test (SRT)), and the storage stability of serum samples at 20 \u00b0C, 4 \u00b0C, and \u221220 \u00b0C. The method showed good precision (intra-assay CVs (coefficient of variations) 3.19\u20139.61%; inter-assay CVs 9.26\u201315.28%). In both the intra- and inter-assays, the highest imprecision was found with the low concentration pool (9.61% and 15.28%) and accuracy (LUD and SRT r2 = 0.99, p &lt; 0.001), while the stability was optimal up until 7 days at \u221220 \u00b0C (\u22127.7%). The method was successfully validated in cats, allowing its future use in diagnostic procedures.<\/jats:p>","DOI":"10.3390\/ani11113100","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T11:47:38Z","timestamp":1635767258000},"page":"3100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2726-6090","authenticated-orcid":false,"given":"Jari","family":"Zambarbieri","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 16900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9708-5148","authenticated-orcid":false,"given":"Pierangelo","family":"Moretti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 16900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8611-8944","authenticated-orcid":false,"given":"Alessia","family":"Giordano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 16900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2316-1929","authenticated-orcid":false,"given":"Paola","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 16900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"194","DOI":"10.2460\/ajvr.2002.63.194","article-title":"Cloning and sequence analysis of the complementary DNA for feline preproparathyroid hormone","volume":"63","author":"Toribio","year":"2002","journal-title":"Am. J. Vet. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/vec.12032","article-title":"The role of phosphorus in the pathophysiology of chronic kidney disease","volume":"23","author":"Geddes","year":"2013","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/1098612X16631234","article-title":"ISFM consensus guidelines on the diagnosis and management of feline chronic kidney disease","volume":"18","author":"Sparkes","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jfms.2003.08.004","article-title":"Disorders of the parathyroid glands","volume":"6","author":"Barber","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1210\/er.2018-00220","article-title":"Clinical guidelines and PTH measurements: Does assay generation matter?","volume":"40","author":"Smit","year":"2019","journal-title":"Endocr. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"594745","DOI":"10.3389\/fendo.2020.594745","article-title":"A rapid intraoperative parathyroid hormone assay based on the immune colloidal gold technique for parathyroid identification in thyroid surgery","volume":"11","author":"Xia","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/j.1748-5827.1993.tb02594.x","article-title":"Measurement of feline intact parathyroid hormone: Assay validation and sample handling studies","volume":"34","author":"Barber","year":"1993","journal-title":"J. Small Anim. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.domaniend.2012.01.003","article-title":"Feline parathyroid hormone: Validation of hormonal assays and dynamics of secretion","volume":"42","author":"Pineda","year":"2012","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1748-5827.2012.01253.x","article-title":"Calcium and phosphate homeostasis in hyperthyroid cats\u2014Associations with development of azotaemia and survival time","volume":"53","author":"Williams","year":"2012","journal-title":"J. Small Anim. Pract."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/1098612X13495234","article-title":"Feline CKD Pathophysiology and risk factors\u2014What do we know?","volume":"15","author":"Reynolds","year":"2013","journal-title":"J. Feline Med. Surg."},{"key":"ref_11","unstructured":"(2021, September 08). IRIS Staging of CKD. Available online: http:\/\/www.iris-kidney.com\/guidelines\/staging.html."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zambarbieri, J., Tagliasacchi, F., Moretti, P., Giordano, A., and Scarpa, P. (2020). Analytical and clinical validation of a new immunoenzymatic method for the measurement of canine parathyroid hormone. Animals, 10.","DOI":"10.3390\/ani10122411"},{"key":"ref_13","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantification","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/vcp.12150","article-title":"Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein","volume":"43","author":"Hagman","year":"2014","journal-title":"Vet. Clin. Pathol."},{"key":"ref_15","first-page":"202","article-title":"Effects of storage temperature and time on clinical biochemical parameters from rat serum","volume":"48","author":"Cray","year":"2009","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1177\/1098612X15581134","article-title":"Feline hyperparathyroidism Pathophysiology, diagnosis and treatment of primary and secondary disease","volume":"17","author":"Parker","year":"2015","journal-title":"J. Feline Med. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1748-5827.1998.tb03613.x","article-title":"Feline chronic renal failure: Calcium homeostasis in 80 cases diagnosed between 1992 and 1995","volume":"39","author":"Barber","year":"1998","journal-title":"J. Small Anim. Pract."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.2460\/javma.241.10.1326","article-title":"Parathyroid hormone concentration in geriatric cats with various degrees of renal function","volume":"241","author":"Finch","year":"2011","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/jvim.12036","article-title":"Fibroblast growth factor 23 (FGF-23) concentrations in cats with early nonazotemic chronic kidney disease (CKD) and in healthy geriatric cats","volume":"27","author":"Finch","year":"2013","journal-title":"J. Vet. Intern. Med."},{"key":"ref_20","unstructured":"(2021, May 26). Guideline on Bioanalytical Method Validation. Available online: https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/guideline-bioanalytical-method-validation_en.pdf."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1939-165X.2002.tb00268.x","article-title":"Detection of parathyroid hormone-related protein in cats with humoral hypercalcemia of malignancy","volume":"31","author":"Bolliger","year":"2002","journal-title":"Vet. Clin. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1111\/j.1748-5827.1999.tb03015.x","article-title":"Nutritional secondary hyperparathyroidism in six cats","volume":"40","author":"Tomsa","year":"1999","journal-title":"J. Small Anim. Pract."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/1098612X20925686","article-title":"Feline plasma adrenocorticotropic hormone: Validation of a chemiluminescent assay and concentrations in cats with hypercortisolism, primary hypoadrenocorticism and other diseases","volume":"23","author":"Tardo","year":"2021","journal-title":"J. Feline Med. Surg."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/11\/3100\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:23:15Z","timestamp":1760167395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/11\/3100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,29]]},"references-count":23,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["ani11113100"],"URL":"https:\/\/doi.org\/10.3390\/ani11113100","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,29]]}}}