{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T07:52:45Z","timestamp":1766908365751,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001044","name":"Dairy Australia (Australia)","doi-asserted-by":"publisher","award":["Flexible Feeding Systems"],"award-info":[{"award-number":["Flexible Feeding Systems"]}],"id":[{"id":"10.13039\/501100001044","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018823","name":"Agriculture Victoria","doi-asserted-by":"publisher","award":["Flexible Feeding Systems"],"award-info":[{"award-number":["Flexible Feeding Systems"]}],"id":[{"id":"10.13039\/501100018823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001782","name":"University of Melbourne","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001782","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001604","name":"Teagasc","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001604","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>To increase the dry matter and metabolisable energy intake of cows, dairy farmers often supplement pasture with concentrates and conserved fodder. Feeding large amounts of highly fermentable concentrates to cows can result in metabolic issues, such as ruminal acidosis, and thus safer but more efficient introduction strategies are desirable. We assessed the role that forages play in ruminal, behavioural and production responses to a wheat grain challenge in dairy cows with no previous wheat adaptation. Multiparous lactating Holstein dairy cows (n = 16) were fed a forage-only diet of either lucerne (Medicago sativa) hay, perennial ryegrass (Lolium perenne L.) hay or one of two cultivars of zero-grazing fresh perennial ryegrass herbage (Bealey or Base), for 3 weeks. The forage diet was then supplemented with crushed wheat grain at 8 kg dry matter\/cow day\u22121, with no adaptation period. Wheat comprised between 32 and 43% of total dry matter intake. Cows fed hay maintained a higher mean ruminal fluid pH than those fed herbage, on both the forage-only diet (6.43 vs. 6.17) and the forage plus wheat diet (6.03 vs. 5.58). Following supplementation of wheat, cows fed herbage exhibited minimum ruminal fluid pH levels indicative of acute ruminal acidosis, at 5.15 and 5.06 for cultivars Bealey and Base, respectively. Furthermore, for both herbage cultivars, adding wheat resulted in a ruminal fluid pH under 6 for &gt;20 h\/day. The ruminal environment of cows fed lucerne hay remained most stable throughout the grain challenge, spending the least amount of time below pH 6.0 (9.0 h\/day). Hay created a ruminal environment that was better able to cope with the accumulation of acid as wheat was digested. A combination of increased ruminating time and a slower rate of fermentation, due to higher neutral detergent fiber and lower metabolisable energy concentrations in the hays, is likely responsible for the higher ruminal fluid pH values. Forage plays a critical role in wheat introduction strategies; aggressive adaptation strategies could be implemented when a hay such as lucerne is used as the base forage.<\/jats:p>","DOI":"10.3390\/ani11113188","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T22:08:41Z","timestamp":1636409321000},"page":"3188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prior Forage Type Influences Ruminal Responses to a Wheat Grain Challenge in Lactating Dairy Cows"],"prefix":"10.3390","volume":"11","author":[{"given":"Victoria M.","family":"Russo","sequence":"first","affiliation":[{"name":"Agriculture Victoria, Ellinbank, VIC 3821, Australia"},{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"},{"name":"Centre for Agricultural Innovation, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"},{"name":"Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, Co., Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian J.","family":"Leury","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"},{"name":"Centre for Agricultural Innovation, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emer","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, Co., Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6708-5393","authenticated-orcid":false,"given":"Murray C.","family":"Hannah","sequence":"additional","affiliation":[{"name":"Agriculture Victoria, Ellinbank, VIC 3821, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin J.","family":"Auldist","sequence":"additional","affiliation":[{"name":"Agriculture Victoria, Ellinbank, VIC 3821, Australia"},{"name":"Centre for Agricultural Innovation, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg L.","family":"Morris","sequence":"additional","affiliation":[{"name":"Agriculture Victoria, Ellinbank, VIC 3821, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Wales","sequence":"additional","affiliation":[{"name":"Agriculture Victoria, Ellinbank, VIC 3821, Australia"},{"name":"Centre for Agricultural Innovation, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3168\/jds.S0022-0302(03)73581-4","article-title":"Invited review: Production and digestion of supplemented dairy cows on pasture","volume":"86","author":"Bargo","year":"2003","journal-title":"J. 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