{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:06:11Z","timestamp":1789383971215,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to assess changes in the integrity of sperm cell membranes during the storage of semen collected from Duroc \u00d7 Pietrain crossbred boars and purebred boars of the component breeds. To compare the cell membrane integrity of sperm heads in crossbred and purebred boars, heterosis effects were estimated. The study was conducted on 48 ejaculates collected from Duroc \u00d7 Pietrain crossbred boars and from purebred Duroc and Pietrain boars used for artificial insemination. Microscope slides were prepared from each ejaculate for the evaluation of the cell membrane integrity of the sperm, at 1, 24, 48, 72, and 96 h after collection of the ejaculate. Diluted ejaculates were stored at 17 \u00b0C. Sperm membrane integrity was analysed by two methods: SYBR-14\/PI and eosin\u2013nigrosin. Our results showed that the cell membrane integrity of sperm heads changed with storage time, but the extent of the changes varied depending on the genetic group of boars. The semen of Duroc \u00d7 Pietrain crossbreds was clearly seen to be less sensitive to storage conditions than that of boars of the parent breeds, which was confirmed by the calculated heterosis effects. The percentage of sperm with an intact cell membrane was higher in crossbred boars than in purebred boars (p \u2264 0.05). In addition, significantly fewer moribund sperm spermatozoa and spermatozoa with a damaged cell membrane were observed in crossbred boars (p \u2264 0.05). In the semen of purebred Duroc and Pietrain boars, the cell membrane integrity of the sperm should be assessed more often during storage than in the semen of Duroc \u00d7 Pietrain crossbred boars. This study provides valuable information for the development and implementation of semen quality monitoring in crossbred boars and boars of the parent breeds during storage at 17 \u00b0C with respect to the cell membrane structure of sperm heads. The evaluation methods used effectively identify damage to the cell membranes of the sperm during semen storage.<\/jats:p>","DOI":"10.3390\/ani11123373","type":"journal-article","created":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T22:19:16Z","timestamp":1638137956000},"page":"3373","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Integrity of Sperm Cell Membrane in the Semen of Crossbred and Purebred Boars during Storage at 17 \u00b0C: Heterosis Effects"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2654-3604","authenticated-orcid":false,"given":"Anna","family":"Wysoki\u0144ska","sequence":"first","affiliation":[{"name":"Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Szablicka","sequence":"additional","affiliation":[{"name":"Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"ref_1","first-page":"278","article-title":"Comparison of in vitro methods for evaluation of boar semen quality","volume":"61","author":"Banys","year":"2005","journal-title":"Pol. Vet. Med."},{"key":"ref_2","first-page":"e63","article-title":"Effect of dilution temperature on boar semen quality","volume":"47","author":"Rijsselaere","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3906\/biy-1412-76","article-title":"Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability","volume":"39","author":"Piasecka","year":"2015","journal-title":"Turk. J. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0378-4320(99)00035-4","article-title":"Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage","volume":"58","author":"Cerolini","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.anireprosci.2017.02.007","article-title":"Long-term liquid storage and reproductive evaluation of an innovative boar semen extender (Formula12\u00ae) containing a non-reducing disaccharide and an enzymatic agent","volume":"180","author":"Bresciani","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1530\/reprod\/118.1.145","article-title":"Permeability of boar and bull spermatozoa to the nucleic acid stains Propidium Iodide or Hoechst 3 or to Eosin: Accuracy in the assessment of cell viability","volume":"118","author":"Pintado","year":"2000","journal-title":"J. Reprod. Fert."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0093-691X(03)00174-2","article-title":"Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate","volume":"60","author":"Brito","year":"2003","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.2047-2927.2013.00045.x","article-title":"The specific response to capacitating stimuli is a sensitive indicator of chilling injury in hypothermically stored boar spermatozoa","volume":"1","author":"Schmid","year":"2013","journal-title":"Andrology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.anireprosci.2017.05.013","article-title":"Impact of different dilution techniques on boar sperm quality and sperm distribution of the extended ejaculate","volume":"182","author":"Schulze","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/j.1439-0531.2006.00733.x","article-title":"Air contact influences the pH of extended porcine semen","volume":"42","author":"Vyt","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/rda.12532","article-title":"Rotation of boar semen doses during storage sffects sperm quality","volume":"50","author":"Schulze","year":"2015","journal-title":"Reprod. Domest. Anim."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-4320(00)00088-9","article-title":"Sexing mammalian sperm for production of offspring: The state of the art","volume":"60\u201361","author":"Johnson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2754\/avb201281020195","article-title":"Comparative analysis of Duroc and Pietrain boar sperm morphology","volume":"81","author":"Kondracki","year":"2012","journal-title":"Acta Vet. Brno"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3409\/fb63_1.9","article-title":"Frequency of Cytoplasmic Droplets Depends on the Breed and Age of Insemination Boars","volume":"63","author":"Banaszewska","year":"2015","journal-title":"Folia Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.livsci.2017.04.013","article-title":"The effect of age, interval collection and season on selected semen parameters and prediction of AI boars productivity","volume":"201","author":"Knecht","year":"2017","journal-title":"Livest. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1002\/j.1939-4640.2001.tb02194.x","article-title":"Morphologically distinct sperm subpopulations defined by Fourier shape descriptors in fresh ejaculates correlate with variation in boar semen quality following cryopreservation","volume":"22","author":"Thurston","year":"2001","journal-title":"J. Androl."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106217","DOI":"10.1016\/j.anireprosci.2019.106217","article-title":"Heterosis for morphometric characteristics of sperm cells from Duroc \u00d7 Pietrain crossbred boars","volume":"211","author":"Kondracki","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"ref_18","first-page":"65","article-title":"Assessment of the effect of heterosis on semen parameters of two-breed crosses of Duroc, Hampshire and Pietrain boars","volume":"56","author":"Kondracki","year":"2013","journal-title":"Arch. Tierz."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.2527\/jas.2008-1373","article-title":"Quantification of factors affecting semen traits in artificial insemination boars from animal model analyses","volume":"87","author":"Wolf","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1002\/mrd.22840","article-title":"Artificial insemination with frozen-thawed boar sperm","volume":"84","author":"Yeste","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_21","first-page":"231","article-title":"Comparative study of different methods for plasma membrane integrity assessment of frozen-thawed boar spermatozoa","volume":"55","author":"Fraser","year":"2011","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.5713\/ajas.15.0095","article-title":"The usefulness of selected physicochemical indices, cell membrane integrity and sperm chromatin structure in assessments of boar semen sensitivity","volume":"28","author":"Kondracki","year":"2015","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_23","first-page":"677","article-title":"Effect of seasonal factors on the ejaculate properties of crossbred Duroc \u00d7 Pietrain and Pietrain \u00d7 Duroc boars as well as purebred Duroc and Pietrain boars","volume":"53","author":"Kondracki","year":"2009","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0378-4320(03)00142-8","article-title":"Differences among breeds and manifestation of heterosis in AI boar sperm output","volume":"80","author":"Smital","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"517","DOI":"10.2478\/pjvs-2013-0072","article-title":"Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17 \u00b0C","volume":"16","author":"Fraser","year":"2013","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1095\/biolreprod53.2.276","article-title":"Viability assessment of the mammalian sperm using SYBR-14 and propidium iodide","volume":"53","author":"Garner","year":"1995","journal-title":"Biol. Reprod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0378-4320(00)00194-9","article-title":"Effects of season and breed on sperm acrosin activity and semen quality of boars","volume":"64","author":"Ciereszko","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_28","first-page":"42","article-title":"Quality of semen of young boars of the breeds Pietrain and Duroc and their reciprocal crosses","volume":"51","author":"Pietruszka","year":"2008","journal-title":"Arch. Tierz."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.anireprosci.2008.01.024","article-title":"Effect influencing boar semen","volume":"110","author":"Smital","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"ref_30","first-page":"765","article-title":"Variability of semen traits of boars used in artificial insemination","volume":"66","author":"Wierzbicki","year":"2010","journal-title":"Med. Wet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/S0093-691X(00)00428-3","article-title":"Assessment of stallion spermatozoa viability by flow cytometry and light microscope analysis","volume":"54","author":"Merkies","year":"2000","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1006\/cryo.1996.0032","article-title":"Effects of phosphatidylserine and cholesterol liposomes on the viability, motility and acrosomal integrity of stallion spermatozoa prior to and after cryopreservation","volume":"33","author":"Wilhelm","year":"1996","journal-title":"Cryobiology"},{"key":"ref_33","first-page":"1022","article-title":"Use of fluorescent staining SYBR-14\/propidium iodide and flow cytometry in evaluating the quality of chilled dog semen","volume":"61","author":"Klimowicz","year":"2005","journal-title":"Pol. Vet. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/j.theriogenology.2011.09.001","article-title":"Comparison of assessment of pigeon sperm viability by kontrast-phase microscope (eosin-nigrosin staining) and flow cytometry (SYBR-14\/propidium iodide (PI) staining) [evaluation of pigeon sperm viability]","volume":"77","author":"Batkowski","year":"2012","journal-title":"Theriogenology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/1751-0147-51-53","article-title":"Assessment of sperm quality traits in relation to fertility in boar semen","volume":"51","author":"Sutkeviciene","year":"2009","journal-title":"Acta Vet. Scand."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7560","DOI":"10.1073\/pnas.78.12.7560","article-title":"Timing of fertilization in mammals: Sperm cholesterol\/phospholipid ratio as a determinant of the capacitation interval","volume":"78","author":"Davis","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-3-319-30567-7_6","article-title":"Lipid Regulation of Acrosome Exocytosis","volume":"220","author":"Cohen","year":"2016","journal-title":"Adv. Anat. Embryol. Cell. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1530\/rep.1.01049","article-title":"Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm","volume":"131","author":"Waterhouse","year":"2006","journal-title":"Reproduction"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0011-2240(90)90009-S","article-title":"Cold-induced ultrastructural changes in bull and boar sperm plasma membranes","volume":"27","author":"Chen","year":"1990","journal-title":"Cryobiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.anireprosci.2018.01.011","article-title":"Effect of production management on semen quality during longterm storage in different European boar studs","volume":"190","author":"Schulze","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1006\/cryo.1994.1028","article-title":"Composition and behawior of head membran\u0119 lipids of fresh and cryopreserved boar sperm","volume":"31","author":"Buhr","year":"1994","journal-title":"Crybiology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.theriogenology.2013.07.026","article-title":"Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions","volume":"80","author":"Schulze","year":"2013","journal-title":"Theriogenology"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/12\/3373\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:35:38Z","timestamp":1760168138000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/12\/3373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":42,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["ani11123373"],"URL":"https:\/\/doi.org\/10.3390\/ani11123373","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]}}}