{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:46:20Z","timestamp":1762325180405,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T00:00:00Z","timestamp":1639958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["16\/RC\/3889"],"award-info":[{"award-number":["16\/RC\/3889"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of this study was to compare the molecular, physiological and microbial effects of mushroom powder (MP), vitamin D2 enriched mushroom powder (MPD2) and zinc oxide (ZnO) in pigs post-weaning. Pigs (four pigs\/pen; 12 pens\/treatment) were assigned to: (1) basal diet (control), (2) basal diet + ZnO, (3) basal diet + MP (2 g\/kg feed) and (4) basal diet + MPD2 (2 g\/kg feed). Zinc oxide supplementation improved the feed intake (p &lt; 0.001); increased the caecal abundance of Lactobacillus (p &lt; 0.05); increased the villus height (p &lt; 0.05) in the duodenum, jejunum and ileum; increased the expression of chemokine interleukin 8 (CXCL8; p &lt; 0.05); and decreased the expression of pro-inflammatory cytokine gene interleukin 6 (IL6; p &lt; 0.05), tumour necrosis factor (TNF; p &lt; 0.05), nutrient transporters peptide transporter 1 (SLC15A1; p &lt; 0.05) and fatty acid binding protein 2 (FABP2; (p &lt; 0.05) in the duodenum. Whereas dietary supplementation with MPD2 improved the gastrointestinal morphology (p &lt; 0.05); increased the total volatile fatty acid concentrations (p &lt; 0.05); increased the expression of anti-inflammatory cytokine gene interleukin 10 (IL10; p &lt; 0.05) and nutrient transporters SLC15A1 (p &lt; 0.05), FABP2 (p &lt; 0.05) and vitamin D receptor (VDR; p &lt; 0.05); and reduced the expression of pro-inflammatory cytokine gene IL6 (p &lt; 0.05), it adversely affected average daily feed intake (ADFI; p &lt; 0.001) and average daily gain (ADG; p &lt; 0.05). Mushroom powder supplementation had a positive impact on gastrointestinal morphology (p &lt; 0.05) and upregulated the expression of nutrient transporters SLC15A1 (p &lt; 0.05) and FABP2 (p &lt; 0.05) and tight junction claudin 1 (CLDN1) (p &lt; 0.05) compared to the controls but had no effect on the expression of inflammatory markers (p &gt; 0.05). Furthermore, MP reduced ADFI (p &lt; 0.01); however, this did not negatively impact the ADG (p &gt; 0.05). In conclusion, MP and MPD2 have limited use as commercial feed additives in replacing ZnO in pig diets as feed intake was reduced post-weaning.<\/jats:p>","DOI":"10.3390\/ani11123603","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T08:43:32Z","timestamp":1639989812000},"page":"3603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of Dietary Supplementation with Mushroom or Vitamin D2-Enriched Mushroom Powders on Gastrointestinal Health Parameters in the Weaned Pig"],"prefix":"10.3390","volume":"11","author":[{"given":"Alison","family":"Dowley","sequence":"first","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2639-395X","authenticated-orcid":false,"given":"Torres","family":"Sweeney","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eadaoin","family":"Conway","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9304-2925","authenticated-orcid":false,"given":"Stafford","family":"Vigors","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supriya","family":"Yadav","sequence":"additional","affiliation":[{"name":"MBio, Monaghan Mushroom Group, Tyholland, Co., H18 FW95 Monaghan, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jude","family":"Wilson","sequence":"additional","affiliation":[{"name":"MBio, Monaghan Mushroom Group, Tyholland, Co., H18 FW95 Monaghan, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Gabrielli","sequence":"additional","affiliation":[{"name":"MBio, Monaghan Mushroom Group, Tyholland, Co., H18 FW95 Monaghan, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0906-4065","authenticated-orcid":false,"given":"John V.","family":"O\u2019Doherty","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s12011-013-9638-3","article-title":"Effects of pharmacological concentrations of dietary zinc oxide on growth of post-weaning pigs: A meta-analysis","volume":"152","author":"Sales","year":"2013","journal-title":"Biol. 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