{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:48:53Z","timestamp":1787165333666,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Progetto antibioticfreebeef (PSR Veneto region)","award":["3556074"],"award-info":[{"award-number":["3556074"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Judicious antimicrobial stewardship in livestock industry is needed to reduce the use of antimicrobials (AMU) and the associated risk of antimicrobial resistance. Biosecurity measures are acknowledged for their role against the spread of diseases and the importance in reducing AMU in different species. However, their effectiveness in beef production has been scarcely considered. The aim of this study was to investigate the effect of the quarantine strategy on AMU in beef cattle. A total of 1206 Charolaise animals in five farms were included in the trial. Roughly half of the animals followed the standard procedure of the fattening cycle (no-quarantine; NO-QUA group) and half followed a 30-day period of quarantine (QUA group) since their arrival. Performance and antimicrobial data were recorded and a treatment incidence 100 (TI100it) per animal was calculated. Penicillins was the most used class of antimicrobials. Differences between groups were significant for males only, with NO-QUA group having greater TI100it (3.76 vs. 3.24; p &lt; 0.05) and lower body weight at slaughter (713.4 vs. 723.7 kg; p &lt; 0.05) than QUA group. Results suggest that quarantine strategy can reduce AMU in males without compromising their performance, whereas further investigation is needed for females.<\/jats:p>","DOI":"10.3390\/ani12010116","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T20:42:16Z","timestamp":1641415336000},"page":"116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Promoting Judicious Antimicrobial Use in Beef Production: The Role of Quarantine"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9418-9710","authenticated-orcid":false,"given":"Matteo","family":"Santinello","sequence":"first","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, VEN, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8571-1295","authenticated-orcid":false,"given":"Alessia","family":"Diana","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, VEN, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"De Marchi","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, VEN, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4412-0587","authenticated-orcid":false,"given":"Federico","family":"Scali","sequence":"additional","affiliation":[{"name":"Sector Diagnostic and Animal Health, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna \u2018Bruno Ubertini\u2019 (IZSLER), 25124 Brescia, LOM, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Bertocchi","sequence":"additional","affiliation":[{"name":"Italian National Reference Center for Animal Welfare (CReNBA), Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna \u2018Bruno Ubertini\u2019 (IZSLER), 25124 Brescia, LOM, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5153-7794","authenticated-orcid":false,"given":"Valentina","family":"Lorenzi","sequence":"additional","affiliation":[{"name":"Italian National Reference Center for Animal Welfare (CReNBA), Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna \u2018Bruno Ubertini\u2019 (IZSLER), 25124 Brescia, LOM, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni Loris","family":"Alborali","sequence":"additional","affiliation":[{"name":"Sector Diagnostic and Animal Health, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna \u2018Bruno Ubertini\u2019 (IZSLER), 25124 Brescia, LOM, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9984-8738","authenticated-orcid":false,"given":"Mauro","family":"Penasa","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, VEN, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.20506\/rst.31.1.2106","article-title":"Use of antimicrobial agents in livestock","volume":"31","author":"Page","year":"2012","journal-title":"Rev. Sci. Tech."},{"key":"ref_2","first-page":"1","article-title":"Antimicrobial resistance: The use of antimicrobials in the livestock sector","volume":"68","author":"Rushton","year":"2014","journal-title":"OECD Food Agric. Fish. Work. Pap."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1093\/jac\/dkt443","article-title":"Correlation between veterinary antimicrobial use and antimicrobial resistance in food-producing animals: A report on seven countries","volume":"69","author":"Chantziaras","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_4","unstructured":"World Health Organization (WHO) (2020, October 22). Strategic and Technical Advisory Group on Antimicrobial Resistance (STAG-AMR): Report of the Second Meeting. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/128675."},{"key":"ref_5","unstructured":"European Medicines Agency (EMA) (2020, October 22). Sales of Veterinary Antimicrobial Agents in 31 European Countries in 2017. (EMA\/294674\/2019). Available online: https:\/\/www.ema.europa.eu\/en\/documents\/report\/sales-veterinary-antimicrobial-agents-31-european-countries-2017_en.pdf."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.1128\/AEM.71.7.3872-3881.2005","article-title":"Effects of subtherapeutic administration of antimicrobial agents to beef cattle on the prevalence of antimicrobial aesistance in Campylobacter jejuni and Campylobacter hyointestinalis","volume":"71","author":"Inglis","year":"2005","journal-title":"Appl. Environ. Microb."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s40104-016-0127-3","article-title":"Antimicrobial usage and resistance in beef production","volume":"7","author":"Cameron","year":"2016","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104934","DOI":"10.1016\/j.prevetmed.2020.104934","article-title":"The effect of tylosin on antimicrobial resistance in beef cattle enteric bacteria: A systematic review and meta-analysis","volume":"176","author":"Cazera","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/2049-1891-4-35","article-title":"Alternatives to antibiotics as growth promoters for use in swine production: A review","volume":"4","author":"Thacker","year":"2013","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.meatsci.2014.06.026","article-title":"Pre- and post-harvest interventions to reduce pathogen contamination in the U.S. beef industry","volume":"98","author":"Wheeler","year":"2014","journal-title":"Meat Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3389\/fvets.2017.00237","article-title":"Antimicrobial resistance: Its surveillance, impact, and alternative management strategies in dairy animals","volume":"4","author":"Sharma","year":"2018","journal-title":"Front. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/zph.12283","article-title":"Reducing antimicrobial usage in pig production without jeopardizing production parameters","volume":"64","author":"Postma","year":"2016","journal-title":"Zoonoses Public Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3389\/fvets.2017.00213","article-title":"Why antibiotic use data in animals needs to be collected and how this can be facilitated","volume":"4","year":"2017","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.prevetmed.2005.10.003","article-title":"Quantification and evaluation of antimicrobial drug use in group treatments for fattening pigs in Belgium","volume":"74","author":"Timmerman","year":"2006","journal-title":"Prev. Vet. Med."},{"key":"ref_15","unstructured":"AACTING (2020, October 22). Guidelines for Collection, Analysis and Reporting of Farm-Level Antimicrobial Use, in the Scope of Antimicrobial Stewardship. Version 1.2_2019-07-02. Available online: https:\/\/aacting.org\/swfiles\/files\/AACTING_Guidelines_V1.2_2019.07.02_54.pdf."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.tvjl.2013.08.029","article-title":"Relationship between biosecurity and production\/antimicrobial treatment characteristics in pig herds","volume":"198","author":"Laanen","year":"2013","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Dewulf, J., and Van Immerseel, F. (2018). General principles of biosecurity in animal production and veterinary medicine. Biosecurity in Animal Production and Veterinary Medicine: From Principles to Practice, ACCO. [1st ed.].","DOI":"10.1079\/9781789245684.0000"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1136\/inp.g2829","article-title":"Biosecurity in the beef herd","volume":"36","author":"Sibley","year":"2014","journal-title":"In Pract."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"167","DOI":"10.3389\/fvets.2020.00167","article-title":"Using biosecurity measures to combat respiratory disease in cattle: The Norwegian control program for bovine respiratory syncytial virus and bovine coronavirus","volume":"7","author":"Stokstad","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_20","first-page":"150","article-title":"Perception, motivators and obstacles of biosecurity in cattle production","volume":"87","author":"Damiaans","year":"2018","journal-title":"Vlaams Diergen. Tijds."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"185","DOI":"10.2460\/javma.2000.217.185","article-title":"Biosecurity practices of beef cow-calf producers","volume":"217","author":"Sanderson","year":"2000","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Dewulf, J., and Van Immerseel, F. (2018). Biosecurity and its relationship with health, production and antimicrobial use. Biosecurity in Animal Production and Veterinary Medicine: From Principles to Practice, ACCO. [1st ed.].","DOI":"10.1079\/9781789245684.0000"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.prevetmed.2016.02.002","article-title":"Antimicrobial drug use and risk factors associated with treatment incidence and mortality in Swiss veal calves reared under improved welfare conditions","volume":"126","author":"Lava","year":"2016","journal-title":"Prev. Vet. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.prevetmed.2019.03.007","article-title":"Effects of management practices, animal transport and barn climate on animal health and antimicrobial use in Swiss veal calf operations","volume":"167","author":"Schnyder","year":"2019","journal-title":"Prev. Vet. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104907","DOI":"10.1016\/j.prevetmed.2020.104907","article-title":"Effects of the novel concept \u2018outdoor veal calf\u2019 on antimicrobial use, mortality and weight gain in Switzerland","volume":"176","author":"Becker","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3285","DOI":"10.4081\/ijas.2014.3285","article-title":"A survey on feedlot performance of purebred and crossbred European young bulls and heifers managed under intensive conditions in Veneto, northeast Italy","volume":"13","author":"Gallo","year":"2014","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4081\/ijas.2005.2s.251","article-title":"Growth performance, cleanliness and lameness of finishing Charolais bulls housed in littered pens of different design","volume":"4","author":"Cozzi","year":"2005","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"128","DOI":"10.4081\/ijas.2005.3s.128","article-title":"Factors affecting growth performance in beef production: An on farm survey","volume":"4","author":"Sturaro","year":"2005","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_29","unstructured":"Currin, J., and Whittie, W.D. (2020, October 22). Recognition and Treatment of Bovine Respiratory Disease Complex. Virginia Cooperative Extension (VCE). Available online: https:\/\/pubs.ext.vt.edu\/400\/400-008\/400-008_pdf."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.3168\/jds.2019-17827","article-title":"Development of a quantitative risk assessment of bovine viral diarrhea virus and bovine herpesvirus-1 introduction in dairy cattle herds to improve biosecurity","volume":"103","author":"Benavides","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100091","DOI":"10.1016\/j.animal.2020.100091","article-title":"Exploring potential risk factors of antimicrobial use in beef cattle","volume":"15","author":"Diana","year":"2021","journal-title":"Animal"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"104936","DOI":"10.1016\/j.prevetmed.2020.104936","article-title":"To what extent does the composition of batches formed at the sorting facility influence the subsequent growth performance of young beef bulls? A French observational study","volume":"176","author":"Herve","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1017\/S1466252314000255","article-title":"The monster we don\u2019t see: Subclinical BRD in beef cattle","volume":"15","author":"Griffin","year":"2014","journal-title":"Anim. Health Res. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.2527\/jas.2006-046","article-title":"Bovine respiratory disease in feedlot cattle: Environmental, genetic, and economic factors","volume":"84","author":"Snowder","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1751-0813.2007.00184.x","article-title":"Feedlot entry characteristics and climate: Their relationship with cattle growth rate, bovine respiratory disease and mortality","volume":"85","author":"Cusack","year":"2007","journal-title":"Aust. Vet. J."},{"key":"ref_36","first-page":"1095","article-title":"The epidemiology of bovine respiratory disease: What is the evidence for predisposing factors?","volume":"51","author":"Taylor","year":"2010","journal-title":"Can. Vet. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.2527\/1999.7751120x","article-title":"Interaction of cattle health\/immunity and nutrition","volume":"77","author":"Galyean","year":"1999","journal-title":"J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"504","DOI":"10.2527\/1996.743504x","article-title":"Effects of dietary energy source and level on performance of newly arrived feedlot calves","volume":"74","author":"Fluharty","year":"1996","journal-title":"J. Anim. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6862","DOI":"10.3168\/jds.2017-12750","article-title":"Management practices for male calves on Canadian dairy farms","volume":"100","author":"Renaud","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.cvfa.2007.01.003","article-title":"Influence of stress and nutrition on cattle immunity","volume":"23","author":"Carroll","year":"2007","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.agsy.2017.04.005","article-title":"Environmental footprint of the integrated France\u2013Italy beef production system assessed through a multi-indicator approach","volume":"155","author":"Berton","year":"2017","journal-title":"Agric. Syst."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cvfa.2006.03.012","article-title":"Transportation of Cattle","volume":"22","author":"Fike","year":"2006","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"180","DOI":"10.15414\/afz.2020.23.mi-fpap.180-189","article-title":"Sources of variation of antimicrobial use in Charolaise and Limousine beef breeds in Veneto region (Italy)","volume":"23","author":"Santinello","year":"2020","journal-title":"Acta Fytotechn. Zootech."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ijantimicag.2015.09.014","article-title":"Antibiotic combination therapy can select for broad-spectrum multidrug resistance in Pseudomonas aeruginosa","volume":"47","author":"Vestergaard","year":"2016","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1054\/drup.2000.0167","article-title":"The impact of antibiotic use on resistance development and persistence","volume":"3","author":"Barbosa","year":"2000","journal-title":"Drug Resist. Updates"},{"key":"ref_46","first-page":"239","article-title":"Dealing with lameness in Italian beef cattle rearing","volume":"20","author":"Compiani","year":"2014","journal-title":"Large Anim. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1017\/S175173111900106X","article-title":"Health behaviour and growth performance of Charolais and Limousin bulls fattened on different types of flooring","volume":"13","author":"Magrin","year":"2019","journal-title":"Animal"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.2527\/jas.2011-4599","article-title":"Associations between the distance traveled from sale barns to commercial feedlots in the United States and overall performance, risk of respiratory disease, and cumulative mortality in feeder cattle during 1997 to 2009","volume":"90","author":"Cernicchiaro","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.livsci.2014.02.002","article-title":"Swiss pig farmers\u2019 perception and usage of antibiotics during the fattening period","volume":"162","author":"Visschers","year":"2014","journal-title":"Livest. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1017\/S1466252318000075","article-title":"Effects of transportation on cattle health and production: A review","volume":"19","author":"Coetzee","year":"2018","journal-title":"Anim. Health Res. Rev."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/1\/116\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:36:11Z","timestamp":1760362571000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/1\/116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,4]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["ani12010116"],"URL":"https:\/\/doi.org\/10.3390\/ani12010116","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,4]]}}}