{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:20:28Z","timestamp":1791663628982,"version":"4.3.4"},"reference-count":72,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T00:00:00Z","timestamp":1641340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005642","name":"University of Sassari","doi-asserted-by":"publisher","award":["FAR 2019"],"award-info":[{"award-number":["FAR 2019"]}],"id":[{"id":"10.13039\/501100005642","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Goji berries show health benefits, although the possible mechanisms of action, including compositional changes in the gut microbiome, are still not fully understood. The aim of this study was to evaluate the effect of Goji berry supplementation on microbiota composition and metabolites in the digestive tracts of rabbits. Twenty-eight New Zealand White rabbits were fed with a commercial feed (control group, C; n = 14) or the same diet supplemented with 3% of Goji berries (Goji group, G; n = 14), from weaning (35 days old) until slaughter (90 days old). At slaughter, samples from the content of the gastrointestinal tracts were collected and analyzed by Next Generation 16S rRNA Gene Sequencing to evaluate the microbial composition. Ammonia and lactic acid were also quantified in caecum. Results showed differences in microbiota composition between the groups for two phyla (Cyanobacteria and Euryarchaeota), two classes (Methanobacteria and Bacilli), five orders, fourteen families, and forty-five genera. Ruminococcaceae (p &lt; 0.05) and Lachnospiraceae (p &lt; 0.01) were more abundant in G than in C group. Lactobacillaceae also showed differences between the two groups, with Lactobacillus as the predominant genus (p = 0.002). Finally, Goji berry supplementation stimulated lactic acid fermentation (p &lt; 0.05). Thus, Goji berry supplementation could modulate gastrointestinal microbiota composition and caecal fermentation.<\/jats:p>","DOI":"10.3390\/ani12010121","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T20:42:16Z","timestamp":1641415336000},"page":"121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Dietary Supplementation with Goji Berries (Lycium barbarum) Modulates the Microbiota of Digestive Tract and Caecal Metabolites in Rabbits"],"prefix":"10.3390","volume":"12","author":[{"given":"Paola","family":"Cremonesi","sequence":"first","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), U.O.S. di Lodi, Via Einstein, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6352-0036","authenticated-orcid":false,"given":"Giulio","family":"Curone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milano, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3901-2354","authenticated-orcid":false,"given":"Filippo","family":"Biscarini","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), U.O.S. di Lodi, Via Einstein, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8081-3332","authenticated-orcid":false,"given":"Elisa","family":"Cotozzolo","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8500-7970","authenticated-orcid":false,"given":"Laura","family":"Menchetti","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences, University of Bologna, Viale G. Fanin 44, 40137 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1270-1627","authenticated-orcid":false,"given":"Federica","family":"Riva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milano, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4791-0514","authenticated-orcid":false,"given":"Maria Laura","family":"Marongiu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna, 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2326-6701","authenticated-orcid":false,"given":"Bianca","family":"Castiglioni","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), U.O.S. di Lodi, Via Einstein, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0828-4610","authenticated-orcid":false,"given":"Olimpia","family":"Barbato","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albana","family":"Munga","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Agricultural University of Tirana, Kod\u00ebr-Kam\u00ebz, 1029 Tirana, Albania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3771-757X","authenticated-orcid":false,"given":"Marta","family":"Castrica","sequence":"additional","affiliation":[{"name":"Department of Health, Animal Science and Food Safety \u201cCarlo Cantoni\u201d, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-4992","authenticated-orcid":false,"given":"Daniele","family":"Vigo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milano, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majlind","family":"Sulce","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Agricultural University of Tirana, Kod\u00ebr-Kam\u00ebz, 1029 Tirana, Albania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alda","family":"Quattrone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella","family":"Agradi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milano, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4750-2801","authenticated-orcid":false,"given":"Gabriele","family":"Brecchia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milano, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1002\/cbdv.201000018","article-title":"Phytochemical and biological studies of lycium medicinal plants","volume":"8","author":"Yao","year":"2011","journal-title":"Chem. 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