{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:53:04Z","timestamp":1778889184117,"version":"3.51.4"},"reference-count":29,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Although several protocols for cryopreserving buck semen are described in the literature, they differ widely in factors such as season and method of semen collection, extender and sperm concentration. Therefore, choosing a protocol that is suitable for a particular on-farm situation can be problematic. In the present study, semen was collected by artificial vagina from seven bucks on a farm located approximately 90 minutes\u2019 drive away from the laboratory, about 6 weeks before the start of the goat breeding season. The semen was immediately extended in warm semen extender containing soy lecithin and was placed in an insulated box with a cold pack for up to 4 h, during semen collection from the remaining bucks and subsequent transport to the laboratory. Following centrifugation at 4 \u00b0C and resuspension in the soy lecithin extender to a sperm concentration of 800 \u00d7 106 spermatozoa\/mL, 0.25 mL plastic straws were filled and frozen in racks 4 cm above the surface of liquid nitrogen. This simple protocol resulted in an acceptable post-thaw quality for all seven bucks, with a mean post-thaw motility of 55 \u00b1 21% and mean fragmented chromatin of 3.27 \u00b1 1.39%. Normal sperm morphology was &gt;90% in all ejaculates. The semen was sent to a gamete bank for long-term storage.<\/jats:p>","DOI":"10.3390\/ani12030352","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T09:59:21Z","timestamp":1643709561000},"page":"352","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Practical Method for Freezing Buck Semen"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5245-7331","authenticated-orcid":false,"given":"Jane M.","family":"Morrell","sequence":"first","affiliation":[{"name":"Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-75007 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5508-4921","authenticated-orcid":false,"given":"Pongpreecha","family":"Malaluang","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-75007 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3588-091X","authenticated-orcid":false,"given":"Theodoros","family":"Ntallaris","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-75007 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Johannisson","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-75007 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.theriogenology.2010.04.014","article-title":"Vaginal deposition of frozen-thawed semen in Norwegian Dairy goats: Comparison of single and double insemination with equal total number of spermatozoa","volume":"74","author":"Nordstoga","year":"2010","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.anireprosci.2010.04.182","article-title":"The effect of cryopreservation on goat semen characteristics related to sperm freezability","volume":"121","author":"Dorado","year":"2010","journal-title":"Anim. Reprod. 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