{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:56:20Z","timestamp":1762520180517,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture and Rural Development of Poland","award":["No. 505.037.07"],"award-info":[{"award-number":["No. 505.037.07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to investigate how the extrusion of faba bean seeds (var. Albus) and\/or the addition of protease to pigs\u2019 diets affected the animals\u2019 growth parameters, digestibility of nutrients, selected physiological parameters of the digestive tract, and biochemical blood parameters. A 28-day experiment was conducted on 32 pigs weighing 9 \u00b1 0.2 kg. The animals were allocated to four treatments in a 2 \u00d7 2 factorial arrangement with the main effects of extrusion (raw or extruded) and effects of protease supplementation (0 and 0.05%). Extrusion reduced the levels of neutral detergent fibre, trypsin inhibitor, phytate-P, and resistant starch but did not improve the digestibility of protein and dry matter in faba bean seeds. The pigs\u2019 growth performance, ileal digestibility, enzyme activity, and morphometric parameters of the ileum were not significantly affected by extrusion, except for a higher feed intake between the 15th and 28th day of the experiment. The protease supplementation gave comparable results as the diet without protease, except the feed conversion ratio (in the periods of 15\u201328th day and 0\u201328th day), which was higher than in the groups without protease. The extrusion and protease increased acetate and acetoacetate contents in the cecal digesta, but propionate, butyrate, and isovalerate concentrations in the digesta of the pigs in this group were lower. Thanks to protease supplementation, protein and oil levels in the diet were reduced, which cut the cost of feed mixtures. The extrusion and protease additive combined together did not improve the pigs\u2019 growth performance in this experiment.<\/jats:p>","DOI":"10.3390\/ani12050563","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T22:54:02Z","timestamp":1645656842000},"page":"563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Effects of Protease Supplementation and Faba Bean Extrusion on Growth, Gastrointestinal Tract Physiology and Selected Blood Indices of Weaned Pigs"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2753-4371","authenticated-orcid":false,"given":"Anita","family":"Zaworska-Zakrzewska","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine and Animal Science, Pozna\u0144 University of Life Sciences, ul. Wo\u0142y\u0144ska 33, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0429-2533","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Kasprowicz-Potocka","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine and Animal Science, Pozna\u0144 University of Life Sciences, ul. Wo\u0142y\u0144ska 33, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaudia","family":"Cio\u0142ek","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Station in Gorzy\u0144, Poznan University of Life Sciences, ul. Wojska Polskiego 28, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7182-6905","authenticated-orcid":false,"given":"Ewa","family":"Pruszy\u0144ska-Oszma\u0142ek","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine and Animal Science, Pozna\u0144 University of Life Sciences, ul. Wo\u0142y\u0144ska 35, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9011-8592","authenticated-orcid":false,"given":"Kinga","family":"Stuper-Szablewska","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Wood Technology, Pozna\u0144 University of Life Sciences, ul. Wojska Polskiego 75, 60-625 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Rutkowski","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine and Animal Science, Pozna\u0144 University of Life Sciences, ul. Wo\u0142y\u0144ska 33, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,23]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Tu\u015bnio, A., Taciak, M., Barszcz, M., \u015awi\u0119ch, E., Bachanek, I., and Skomia\u0142, J. (2017). Effect of replacing soybean meal by raw or extruded pea seeds on growth performance and selected physiological parameters of the ileum and distal colon of pigs. 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