{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:45:20Z","timestamp":1762508720312,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014434","name":"Polish National Agency for Academic Exchange","doi-asserted-by":"publisher","award":["PPI\/APM\/2019\/1\/00044\/U\/00001"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Populations of many galliform species have declined mainly due to habitat loss and over-hunting, notably the Congo peacock, which has been classified as a vulnerable species by the International Union for Conservation of Nature (IUCN). The domestic turkey, being a species of least concern, which has been reported to be closely related to peacocks, could serve as a model for the optimization of assisted reproductive technologies for the Congo peacock. This study was aimed at developing a suitable turkey semen extender for artificial insemination in field conditions. Semen was collected using the dorso-abdominal massage technique from seven turkey toms and analyzed. Ejaculates with &gt;70% motility and &gt;80% live spermatozoa were pooled and divided into four aliquots (four treatments). Each of the four treatments was extended in a soybean-based extender or an egg yolk-based extender, with or without L-ascorbic acid. Two liquid preservation protocols (ambient temperature (35 \u00b0C) and chilled (4 \u00b0C)) were employed, and quality parameters including motility, viability and morphology were evaluated. The results show that the two extenders were similar with regard to semen quality parameters, and L-ascorbic acid supplementation of the turkey semen extenders improved semen quality during liquid storage.<\/jats:p>","DOI":"10.3390\/ani12050648","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T20:36:30Z","timestamp":1646339790000},"page":"648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Spermatozoa Survival in Egg Yolk-Based and Soybean-Based Extenders at Ambient and Chilling Temperature in Domestic Turkeys (Meleagris gallopavo)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8477-1368","authenticated-orcid":false,"given":"Isa Mohammed","family":"Alkali","sequence":"first","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri 600230, Nigeria"},{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suleiman Omeiza","family":"Asuku","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri 600230, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1436-1985","authenticated-orcid":false,"given":"Martina","family":"Colombo","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Modu","family":"Bukar","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri 600230, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed Ahmed","family":"Waziri","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri 600230, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9598-9098","authenticated-orcid":false,"given":"Gaia Cecilia","family":"Luvoni","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s40657-018-0124-7","article-title":"Research Advances of Galliformes since 1990 and Future Prospects","volume":"9","author":"Tian","year":"2018","journal-title":"Avian Res."},{"key":"ref_2","unstructured":"Hennache, A. (2007, January 14\u201321). A Review of Captive Galliformes in European Zoos. Proceedings of the 4th International Galliformes Symposium, Chengdu, China."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Vi\u00e9, J.C., Hilton-Taylor, C., and Stuart, S.N. (2009). Wildlife in a Changing World: An Analysis of the 2008 IUCN Red List of Threatened Species, International Union for Conservation of Nature and Resources.","DOI":"10.2305\/IUCN.CH.2009.17.en"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3354\/esr00668","article-title":"Assessment of Climatically Suitable Area for Syrmaticus Reevesii under Climate Change","volume":"28","author":"Feng","year":"2015","journal-title":"Endanger. Species Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1080\/00071660701285897","article-title":"Characterisation of Peacock (Pavo Cristatus) Mitochondrial 12s Rrna Sequence and Its Use in Differentiation from Closely Related Poultry Species","volume":"48","author":"Saini","year":"2007","journal-title":"Br. Poult. Sci."},{"key":"ref_6","unstructured":"Bakst, M.R., and Dymond, J.S. (2011). Artificial Insemination in Poultry. Success in Artificial Insemination\u2014Quality of Semen and Diagnostics Employed, InTech."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-4320(00)00160-3","article-title":"Storage of Poultry Semen","volume":"62","author":"Donoghue","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gordon, I.R. (2004). Reproductive Technologies in Farm Animals, CABI Publishers.","DOI":"10.1079\/9780851998626.0000"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1016\/j.theriogenology.2004.07.020","article-title":"Gelatinases and Serine Proteinase Inhibitors of Seminal Plasma and the Reproductive Tract of Turkey (Meleagris gallopavo)","volume":"63","author":"Kowalski","year":"2005","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.3382\/ps.0632084","article-title":"Detection and Incidence of Yellow Turkey Semen on Commercial Breeder Farms","volume":"63","author":"Hess","year":"1984","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.3382\/ps\/pey490","article-title":"Expression and Secretion of Albumin in Male Turkey (Meleagris gallopavo) Reproductive Tract in Relation to Yellow Semen Syndrome","volume":"98","author":"Hejmej","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"513","DOI":"10.4081\/ijas.2005.2s.513","article-title":"Comparison of Different Extenders on the Quality Characteristics of Turkey Semen During Storage","volume":"4","author":"Iaffaldano","year":"2005","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.3382\/ps.2011-02028","article-title":"Use of Phosphatidylcholine to Improve the Function of Turkey Semen Stored at 4 \u00b0C for 24 Hours","volume":"91","author":"Long","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1080\/00071667608416264","article-title":"Studies on the Dilution of Turkey Semen","volume":"17","author":"Sexton","year":"1976","journal-title":"Br. Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.3382\/ps\/pey209","article-title":"Mitochondrial Membrane Potential and Reactive Oxygen Species in Liquid Stored and Cryopreserved Turkey (Meleagris gallopavo) Spermatozoa","volume":"97","author":"Liszewska","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.theriogenology.2008.09.019","article-title":"Implementing Artificial Insemination as an Effective Tool for Ex Situ Conservation of Endangered Avian Species","volume":"71","author":"Blanco","year":"2009","journal-title":"Theriogenology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.3382\/ps.0621305","article-title":"Beltsville Poultry Semen Extender. 9. Effect of Storage Temperature on Turkey Semen Held Eighteen Hours","volume":"62","author":"Giesen","year":"1983","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1530\/jrf.0.0600121","article-title":"Fertilizing Capacity and Morphology of Fowl and Turkey Spermatozoa in Hypotonic Extender","volume":"60","author":"Bakst","year":"1980","journal-title":"Reproduction"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3382\/ps.0570277","article-title":"A New Poultry Semen Extender: 2. Effect of the Diluent Components on the Fertilizing Capacity of Chicken Semen Stored at 5 \u00b0C","volume":"57","author":"Sexton","year":"1978","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1051\/rnd:19840206","article-title":"Effect of Aeration on the Fertilising Ability of Turkey Semen Stored for 48 Hours at 5 and 15 \u00b0C: A Study from the 33rd to the 47th Week of Age","volume":"24","author":"Lake","year":"1984","journal-title":"Reprod. Nutr. Develop."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/00071688108447908","article-title":"The Effect of Continuous Aeration on the Fertility of Fowl and Turkey Semen Stored above 0 \u00b0C","volume":"22","author":"Wishart","year":"1981","journal-title":"Br. Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.anireprosci.2007.11.024","article-title":"The Preservability of Turkey Semen Quality During Liquid Storage in Relation to Strain and Age of Males","volume":"109","author":"Iaffaldano","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/S0093-691X(01)00683-5","article-title":"Effect of Different Extenders and Storage Temperatures on Sperm Viability of Liquid Ram Semen","volume":"57","author":"Paulenz","year":"2002","journal-title":"Theriogenology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1530\/jrf.0.0700145","article-title":"Metabolism of Fowl and Turkey Spermatozoa at Low Temperatures","volume":"70","author":"Wishart","year":"1984","journal-title":"Reproduction"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/S0093-691X(03)00259-0","article-title":"Bull Semen in Vitro Fertility after Cryopreservation Using Egg Yolk LDL: A Comparison with Optidyl\u00ae, a Commercial Egg Yolk Extender","volume":"61","author":"Amirat","year":"2004","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/S0093-691X(02)00682-9","article-title":"Low Density Lipoproteins Extracted from Hen Egg Yolk by an Easy Method: Cryoprotective Effect on Frozen-Thawed Bull Semen","volume":"57","author":"Moussa","year":"2002","journal-title":"Theriogenology"},{"key":"ref_27","first-page":"131","article-title":"Comparison of Egg Yolks from Three Avian Species in Extender for Cryopreservation of Sahiwal Bull Epididymal Spermatozoa","volume":"29","author":"Akhter","year":"2011","journal-title":"Anim. Sci. Pap. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.cryobiol.2012.06.008","article-title":"Cryoprotective and Contraceptive Properties of Egg Yolk as an Additive in Rooster Sperm Diluents","volume":"65","author":"Coloma","year":"2012","journal-title":"Cryobiology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1016\/j.theriogenology.2015.02.002","article-title":"Sperm\u2013Egg Penetration Assay Assessment of the Contraceptive Effects of Glycerol and Egg Yolk in Rooster Sperm Diluents","volume":"83","author":"Abouelezz","year":"2015","journal-title":"Theriogenology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1002\/mrd.20565","article-title":"New Insights Towards Understanding the Mechanisms of Sperm Protection by Egg Yolk and Milk","volume":"73","author":"Bergeron","year":"2006","journal-title":"Mol. Reprod. Dev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.theriogenology.2018.05.013","article-title":"Effect of Egg Yolk Plasma and Soybean Lecithin on Rooster Frozen-Thawed Sperm Quality and Fertility","volume":"116","author":"Mehdipour","year":"2018","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.theriogenology.2018.06.015","article-title":"Cryoprotectant Effects of Egg Yolk on Indian Red Jungle Fowl (Gallus gallus Murghi) Sperm","volume":"119","author":"Rakha","year":"2018","journal-title":"Theriogenology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.apjr.2016.10.011","article-title":"Substitution of Egg Yolk with Different Concentrations of Soybean Lecithin in Tris-Based Extender During Bulls\u2019 Semen Preservability","volume":"5","year":"2016","journal-title":"Asian Pac. J. Reprod."},{"key":"ref_34","first-page":"149","article-title":"In Vitro Comparison of Soybean Lecithin Based-Extender with Commercially Available Extender for Ram Semen Cryopreservation","volume":"3","author":"Sharafi","year":"2009","journal-title":"Int. J. Fertil. Steril."},{"key":"ref_35","first-page":"185","article-title":"Effect of Soybean Based Extenders on Sperm Parameters of Holstein-Friesian Bull During Liquid Storage at 4 \u00b0C","volume":"46","author":"Rehman","year":"2014","journal-title":"Pak. J. Zool."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/S0093-691X(02)01178-0","article-title":"Fertility of Ram Semen Frozen in Bioxcell\u00ae and Used for Cervical Artificial Insemination","volume":"59","author":"Gil","year":"2003","journal-title":"Theriogenology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0378-4320(00)00161-5","article-title":"Hygienic Aspects of Storage and Use of Semen for Artificial Insemination","volume":"62","author":"Thibier","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1002\/mrd.10347","article-title":"Roles of Antioxidants on Prolonged Storage of Avian Spermatozoa in Vivo and in Vitro","volume":"66","author":"Surai","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s00580-010-1145-8","article-title":"Effect of Ascorbic Acid on Chicken Semen Quality During Liquid Storage","volume":"21","author":"Tabatabaei","year":"2012","journal-title":"Comp. Clin. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3382\/ps.0160019","article-title":"The Collection of Spermatozoa from the Domestic Fowl and Turkey","volume":"16","author":"Burrows","year":"1937","journal-title":"Poult. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.theriogenology.2005.01.012","article-title":"Spermatogenesis in the Turkey (Meleagris gallopavo): Quantitative Approach in Mature and Adult Males Subjected to Various Photoperiods","volume":"65","author":"Noirault","year":"2006","journal-title":"Theriogenology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1111\/j.1439-0531.2011.01928.x","article-title":"Comparative Quality Assessment of Buffalo (Bubalus Bubalis) Semen Chilled (5 \u00b0C) in Egg Yolk- and Soya Milk\u2013Based Extenders","volume":"47","author":"Singh","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e15553","DOI":"10.1111\/jfpp.15553","article-title":"Emulsifying Properties of Plasma Fractionated from Egg Yolk Using Low Centrifugal Forces-Mayonnaise Preparation","volume":"45","author":"Ali","year":"2021","journal-title":"J. Food Process. Preserv."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.3382\/ps.2011-01355","article-title":"Semen Cryopreservation for the Creation of a Spanish Poultry Breeds Cryobank: Optimization of Freezing Rate and Equilibration Time","volume":"90","author":"Coloma","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1080\/00071660500098210","article-title":"Effect of Collection Frequency on Quantitative and Qualitative Characteristics of Pigeon (Columba livia) Semen","volume":"46","author":"Klimowicz","year":"2005","journal-title":"Br. Poult. Sci."},{"key":"ref_46","unstructured":"Macpherson, M.L. (2001, January 28). How to Evaluate Semen in the Field. Proceedings of the Annual Convention of the AAEP, San Diego, CA, USA."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Agarwal, A., Gupta, S., and Sharma, R. (2016). Eosin-Nigrosin Staining Procedure. Andrological Evaluation of Male Infertility: A Laboratory Guide, Springer International Publishing.","DOI":"10.1007\/978-3-319-26797-5"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.14202\/vetworld.2014.1117-1122","article-title":"Semen Quality Parameters, Their Inter-Relationship and Post-Washing Sperm Attributes of Rhode Island Red Roosters","volume":"7","author":"Churchil","year":"2014","journal-title":"Vet. World"},{"key":"ref_49","first-page":"1087","article-title":"Morphological Defects in Turkey Semen","volume":"26","author":"Alkan","year":"2002","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3923\/ijps.2005.217.221","article-title":"Effect of Frequency of Ejaculation on Semen Characteristics in Two Breeds of Turkeys (Meleagris gallopavo) Raised in Tropical Environment","volume":"4","author":"Zahradden","year":"2005","journal-title":"Int. J. Poult. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.5897\/AJMR2013.5906","article-title":"Semen and Microbial Characteristics of Two Breeds of Turkeys in an Arid Tropical Environment of Bauchi State, Nigeria","volume":"8","author":"Ngu","year":"2014","journal-title":"Afr. J. Microbiol."},{"key":"ref_52","first-page":"67","article-title":"A Preliminary Study on Semen Collection, Evaluation and Insemination in Nigerian Local Turkeys (Meleagris gallopavo)","volume":"11","author":"Yahaya","year":"2013","journal-title":"Sokoto J. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1051\/rnd:19810808","article-title":"Changes in the Characteristics of Turkey Ejaculated Semen and Ductus Deferens Semen with Repeated Ejaculations","volume":"21","author":"Bakst","year":"1981","journal-title":"J. Reprod. Nutr. Dev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1093\/ps\/76.10.1440","article-title":"Effects of Water- and Lipid-Soluble Antioxidants on Turkey Sperm Viability, Membrane Integrity, and Motility During Liquid Storage","volume":"76","author":"Donoghue","year":"1997","journal-title":"Poult. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1095\/biolreprod63.5.1450","article-title":"Changes in Turkey Semen Lipids During Liquid in vitro Storage","volume":"63","author":"Douard","year":"2000","journal-title":"Biol. Reprod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1006\/bbrc.2000.3766","article-title":"Induction of Acrosomal Exocytosis in Chicken Spermatozoa by Inner Perivitelline-Derived N-Linked Glycans","volume":"278","author":"Horrocks","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1530\/REP-08-0094","article-title":"A Reappraisal of the Factors Involved in in Vitro Initiation of the Acrosome Reaction in Chicken Spermatozoa","volume":"136","author":"Lemoine","year":"2008","journal-title":"Reproduction"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2016.04.013","article-title":"Cryopreservation of Bull Semen: Evolution from Egg Yolk Based to Soybean Based Extenders","volume":"172","author":"Layek","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0093-691X(02)01369-9","article-title":"In Vitro and in Vivo Comparison of Egg Yolk-Based and Soybean Lecithin-Based Extenders for Cryopreservation of Bovine Semen","volume":"60","author":"Aires","year":"2003","journal-title":"Theriogenology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1262\/jrd.20004","article-title":"Fertility after Artificial Insemination Using a Soybean-Based Semen Extender in Sheep","volume":"54","author":"Fukui","year":"2008","journal-title":"J. Reprod. Dev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.cryobiol.2016.05.010","article-title":"Fertility and Flow Cytometry Study of Frozen-Thawed Sperm in Cryopreservation Medium Supplemented with Soybean Lecithin","volume":"73","author":"Masoudi","year":"2016","journal-title":"Cryobiology"},{"key":"ref_62","first-page":"170","article-title":"Effect of Vitamin a, C & E on Quality of Fowl Semen Stored for 24 Hours at 4 \u00b0C","volume":"7","year":"2002","journal-title":"Iraqi J. Agric."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.anireprosci.2010.04.180","article-title":"The Cryoprotective Effects of Ascorbic Acid Supplementation on Bovine Semen Quality","volume":"121","author":"Hu","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"ref_64","first-page":"384","article-title":"Influence of Ascorbic Acid Addition to the Extender on Dog Sperm Motility, Viability and Acrosomal Integrity During Cold Storage","volume":"158","author":"Ceylan","year":"2007","journal-title":"Vet. Med. Rev."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0093-691X(93)90219-U","article-title":"Effect of Natural Antioxidants on Frozen Bovine Semen Preservation","volume":"40","author":"Beconi","year":"1993","journal-title":"Theriogenology"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1095\/biolreprod65.4.1102","article-title":"Analysis of Reactive Oxygen Species Generating Systems in Rat Epididymal Spermatozoa1","volume":"65","author":"Vernet","year":"2001","journal-title":"Biol. Reprod."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.theriogenology.2010.09.021","article-title":"Incidence, Structure and Morphological Classification of Abnormal Sperm in the Emu (Dromaius novaehollandiae)","volume":"75","author":"Soley","year":"2011","journal-title":"Theriogenology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/rda.13731","article-title":"Effect of Semen Extenders and Storage Time on Quality of Muscovy Duck (Cairina moschata) Drake Semen During the Entire Reproductive Season","volume":"55","author":"Jerysz","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1095\/biolreprod32.4.884","article-title":"Reduction of the Spin-Label Tempone by Ubiquinol in the Electron Transport Chain of Intact Rabbit Spermatozoa1","volume":"32","author":"Chapman","year":"1985","journal-title":"Biol. Reprod."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1042\/bj1980273","article-title":"Oxygen Metabolism of Mammalian Spermatozoa. Generation of Hydrogen Peroxide by Rabbit Epididymal Spermatozoa","volume":"198","author":"Holland","year":"1981","journal-title":"Biochem. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1210\/jc.2007-2616","article-title":"Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa","volume":"93","author":"Koppers","year":"2008","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3382\/japr.2007-00048","article-title":"Effect of Semen Extenders and Storage Time on Sperm Morphology of Four Chicken Breeds","volume":"17","year":"2008","journal-title":"J. Appl. Poult. 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