{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:22:34Z","timestamp":1790281354328,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n Seneca","doi-asserted-by":"publisher","award":["19894\/GERM\/15"],"award-info":[{"award-number":["19894\/GERM\/15"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Juan de la Cierva Formaci\u00f3n, Ministerio de Econom\u00eda y Competitividad","award":["FJC2019-042475-I"],"award-info":[{"award-number":["FJC2019-042475-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Changes in the oxidative status of the blood of horses suffering from gastric ulcers and colic of intestinal aetiology (CIE) have been reported. However, saliva can also be a source of biomarkers of oxidative status. Therefore, this study aims to validate automated assays for the measurement of oxidative status biomarkers (ferric reducing ability of saliva\/serum\u2014FRAS\/FRAP, cupric reducing antioxidant capacity\u2014CUPRAC, the Trolox equivalent antioxidant capacity\u2014TEAC, uric acid, and advanced oxidation protein products\u2014AOPP) in the saliva and serum of horses, to assess their changes in the different ulcer gastric diseases (squamous\u2014ESGD and glandular\u2014EGGD) and CIE, and to evaluate their relationship with serum amyloid A (SAA), adenosine deaminase (ADA), and the systemic inflammatory response syndrome (SIRS) status. The assays showed a low imprecision and good linearity with enough sensitivity in both fluids. In EGGD, higher levels of FRAS, uric acid, and AOPP in saliva were observed compared to the healthy group, correlating with the salivary ADA levels. Horses with CIE showed increases in uric acid concentrations in serum associated with their SIRS status and outcome of the disease. In conclusion, analytes related to the oxidative status can be measured in the saliva and serum from horses by automated assays, and some of them can potentially be assessed as biomarkers in horses with gastric ulcers and CIE.<\/jats:p>","DOI":"10.3390\/ani12050667","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T10:21:16Z","timestamp":1646648476000},"page":"667","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study"],"prefix":"10.3390","volume":"12","author":[{"given":"Mar\u00eda Dolores","family":"Contreras-Aguilar","sequence":"first","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9293-4147","authenticated-orcid":false,"given":"Camila Peres","family":"Rubio","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain"},{"name":"Department of Animal and Food Science, School of Veterinary Science, Universitat Aut\u00f2noma de Barcelona, 08193 Cerdanyola del Vall\u00e8s, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Guillermo","family":"Gonz\u00e1lez-Arostegui","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Mart\u00edn-Cuervo","sequence":"additional","affiliation":[{"name":"Medicine Animal, Faculty of Veterinary Medicine of C\u00e1ceres, University of Extremadura, Avenida de la Universidad S-N, 10002 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose J.","family":"Cer\u00f3n","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0335-482X","authenticated-orcid":false,"given":"Ignacio","family":"Ayala","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida-Marie Holm","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-4576","authenticated-orcid":false,"given":"Stine","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanni","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tvarijonaviciute, A., Mart\u00ednez-Subiela, S., L\u00f3pez-Jornet, P., and Lamy, E. (2020). Saliva in Health and Disease the Present and Future of a Unique Sample for Diagnosis, Springer. [1st ed.].","DOI":"10.1007\/978-3-030-37681-9"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.1016\/j.jprot.2012.05.007","article-title":"Saliva proteomics as an emerging, non-invasive tool to study livestock physiology, nutrition and diseases","volume":"75","author":"Lamy","year":"2012","journal-title":"J. Proteom."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Contreras-Aguilar, M., Escribano, D., Mart\u00ednez-Subiela, S., Mart\u00edn-Cuervo, M., Lamy, E., Tecles, F., and Cer\u00f3n, J.J. (2019). Changes in saliva analytes in equine acute abdominal disease: A sialochemistry approach. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-1933-6"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Contreras-Aguilar, M.D., Tvarijonaviciute, A., Monkeviciene, I., Mart\u00edn-Cuervo, M., Gonz\u00e1lez-Arostegui, L.G., Franco-Mart\u00ednez, L., Cer\u00f3n, J.J., Tecles, F., and Escribano, D. (2020). Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: An analytical and clinical validation pilot study. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02574-2"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.tvjl.2014.08.032","article-title":"The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation","volume":"202","author":"Jacobsen","year":"2014","journal-title":"Vet. J."},{"key":"ref_6","first-page":"144","article-title":"Biomarkers of oxidative stress in saliva in pigs: Analytical validation and changes in lactation","volume":"15","author":"Rubio","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.rvsc.2018.12.015","article-title":"Biomarkers of oxidative stress in saliva of sheep: Analytical performance and changes after an experimentally induced stress","volume":"123","author":"Rubio","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.tvjl.2016.08.004","article-title":"Monitoring acute equine visceral pain with the Equine Utrecht University Scale for Composite Pain Assessment (EQUUS-COMPASS) and the Equine Utrecht University Scale for Facial Assessment of Pain (EQUUS-FAP): A scale-construction study","volume":"216","author":"VanDierendonck","year":"2015","journal-title":"Vet. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102853","DOI":"10.1016\/j.jevs.2019.102853","article-title":"The Contribution of Specific and Nonspecific Biomarkers in Diagnosis of Equine Gastric Ulcer Syndrome (EGUS) Under Field Condition","volume":"84","author":"Shawaf","year":"2020","journal-title":"J. Equine Vet. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1111\/jvim.13578","article-title":"European College of Equine Internal Medicine Consensus Statement-Equine Gastric Ulcer Syndrome in Adult Horses","volume":"29","author":"Sykes","year":"2015","journal-title":"J. Vet. Intern. Med."},{"key":"ref_11","first-page":"69","article-title":"Equine glandular gastric disease: Prevalence, impact and management strategies","volume":"10","author":"Banse","year":"2019","journal-title":"Vet. Med. Res. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105285","DOI":"10.1016\/j.prevetmed.2021.105285","article-title":"Evaluation of a questionnaire to detect the risk of developing ESGD or EGGD in horses","volume":"188","author":"Busechian","year":"2021","journal-title":"Prev. Vet. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"482","DOI":"10.2746\/042516409X391060","article-title":"Initial investigation of mortality rates and prognostic indicators in horses with colic in Israel: A retrospective study","volume":"41","author":"Sutton","year":"2009","journal-title":"Equine Vet. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.cveq.2017.11.006","article-title":"Advances in Diagnostics and Treatments in Horses with Acute Colic and Postoperative Ileus","volume":"34","author":"Burke","year":"2018","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.cveq.2014.04.001","article-title":"Evaluation of the Colic in Horses. Decision for Referral","volume":"30","author":"Cook","year":"2014","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.tvjl.2007.07.033","article-title":"The oxidant\/antioxidant equilibrium in horses","volume":"177","author":"Kirschvink","year":"2008","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.tvjl.2015.03.015","article-title":"Serum amyloid A, haptoglobin, and ferritin in horses with colic: Association with common clinicopathological variables and short-term outcome","volume":"205","author":"Dondi","year":"2015","journal-title":"Vet. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1111\/jvim.14670","article-title":"Prognostic value and development of a scoring system in horses with systemic inflammatory response syndrome","volume":"31","author":"Roy","year":"2017","journal-title":"J. Vet. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1136\/bmj.2.6154.1751-a","article-title":"Adenosine deaminase activity in pleural effusions: An aid to differential diagnosis","volume":"2","author":"Piras","year":"1978","journal-title":"Br. Med. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1378\/chest.97.3.605","article-title":"Serum and pleural adenosine deaminase. Correlation with lymphocytic populations","volume":"97","author":"Pego","year":"1990","journal-title":"Chest"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1111\/jvim.15987","article-title":"Interobserver and intraobserver reliability for 2 grading systems for gastric ulcer syndrome in horses","volume":"35","author":"Wise","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1111\/evj.13066","article-title":"Salivary alpha-amylase activity and concentration in horses with acute abdominal disease: Association with outcome","volume":"51","author":"Tecles","year":"2019","journal-title":"Equine Vet. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.rvsc.2020.01.006","article-title":"Effect of food contamination and collection material in the measurement of biomarkers in saliva of horses","volume":"129","author":"Luisa","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.rvsc.2019.08.004","article-title":"Application of a score for evaluation of pain, distress and discomfort in pigs with lameness and prolapses: Correlation with saliva biomarkers and severity of the disease","volume":"126","author":"Escribano","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Contreras-Aguilar, M.D., Vallejo-mateo, P.J., Lamy, E., Cer, J.J., Tecles, F., and Rubio, C.P. (2021). Changes in Saliva Analytes in Dairy Cows during Peripartum: A Pilot Study. Animals, 11.","DOI":"10.3390\/ani11030749"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1071\/RD10268","article-title":"Relationship between late embryonic mortality and the increase in plasma advanced oxidised protein products (AOPP) in dairy cows","volume":"23","author":"Celi","year":"2011","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.tvjl.2005.04.021","article-title":"Evaluation of a commercially available human serum amyloid A (SAA) turbidometric immunoassay for determination of equine SAA concentrations","volume":"172","author":"Jacobsen","year":"2006","journal-title":"Vet. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3758\/BF03193146","article-title":"G*Power: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences","volume":"39","author":"Faul","year":"2007","journal-title":"Behav. Res. Methods"},{"key":"ref_29","unstructured":"Hinkle, D.E., Wiersma, W., and Jurs, S.G. (2003). Applied Statistics for the Behavioral Sciences, Houghton Mifflin. [5th ed.]."},{"key":"ref_30","unstructured":"(2001). Food and Drug Administration Guidance for Industry: Bioanalytical Method Validation."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3109\/08923973.2010.514917","article-title":"Biomarkers of oxidative stress in ruminant medicine","volume":"33","author":"Celi","year":"2011","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_32","first-page":"303","article-title":"Adenosine deaminase and nucleoside phosphorylase activities in normal human blood mononuclear cell subpopulations","volume":"42","author":"MacDermott","year":"1980","journal-title":"Clin. Exp. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.tvjl.2018.04.018","article-title":"Evaluation of adenosine deaminase in saliva and serum, and salivary \u03b1-amylase, in canine pyometra at diagnosis and after ovariohysterectomy","volume":"236","author":"Tecles","year":"2018","journal-title":"Vet. J."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tvarijonaviciute, A., Mart\u00ednez-Subiela, S., L\u00f3pez-Jornet, P., and Lamy, E. (2020). Salivary Glands\u2019 Anatomy and Physiology. Saliva in Health and Disease: The Present and Future of a Unique Sample for Diagnosis, Springer International Publishing.","DOI":"10.1007\/978-3-030-37681-9"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.jevs.2014.08.002","article-title":"Oxidative stress associated with spasmodic, flatulent, and impaction colic in draft horses","volume":"34","author":"Ibrahim","year":"2014","journal-title":"J. Equine Vet. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1177\/0885066618772825","article-title":"High-Density Lipoprotein, Mean Platelet Volume, and Uric Acid as Biomarkers for Outcomes in Patients With Sepsis: An Observational Study","volume":"35","author":"Chiou","year":"2020","journal-title":"J. Intensive Care Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/5\/667\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:33:27Z","timestamp":1760135607000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/5\/667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,7]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["ani12050667"],"URL":"https:\/\/doi.org\/10.3390\/ani12050667","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,7]]}}}