{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:09:51Z","timestamp":1787314191111,"version":"build-2736575974"},"reference-count":56,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T00:00:00Z","timestamp":1647561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Understanding changes in biometric indices and metabolism in fish exposed to feed deprivation may be useful in aquaculture. The present study elucidates the effect of feed deprivation on physio-biochemical responses, such as changes in biometric indices, nutrient mobilization patterns, and enzyme activities in rohu (Labeo rohita). Experimental fish (av. wt. 3.41 \u00b1 0.07 g) were deprived of feed and sampled at intervals of 0, 15, 30, 45, and 60 days to measure weight, length, body composition, and the activities of enzymes involved in digestion, metabolism, and antioxidation. A decrease in body weight, condition factor (CF), hepatosomatic index (HSI), and gastrosomatic index (GSI) was observed during the initial stage of feed deprivation (15 to 30 days) but remained unchanged thereafter. The total carbohydrate and lipid content also decreased rapidly up until 30 days, then stabilized. However, the reduction in tissue protein content (% wet weight) continued gradually with the duration of feed deprivation from 12.85 \u00b1 0.36 at 0 days to 10.04 \u00b1 0.67 at 15 days, 8.79 \u00b1 0.59 at 30 days, 6.95 \u00b1 0.69 at 45 days, and 6.16 \u00b1 0.8 at 60 days, which was lower, compared to the other two body constituents. Amylase, protease. and lipase activities significantly reduced up until 30 days, but then stabilized. Although G6PDH enzyme activity decreased, gluconeogenic (LDH, AST, and ALT) and antioxidative (SOD and catalase) enzyme activities increased during initial feed deprivation (up to 30 days). A tissue-specific difference in amino acid metabolism with a major role of ALT in liver and AST in muscle was observed. This study revealed that rohu fingerlings adapted well to feed deprivation up until 30 days, beyond which there was an overall deterioration in the metabolic functions.<\/jats:p>","DOI":"10.3390\/ani12060769","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:25:00Z","timestamp":1647811500000},"page":"769","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biometric Indices, Physio-Metabolic Responses and Carcass Quality in Rohu (Labeo rohita) during Feed Deprivation"],"prefix":"10.3390","volume":"12","author":[{"given":"Sona","family":"Yengkokpam","sequence":"first","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"},{"name":"Regional Centre, ICAR-Central Inland Fisheries Research Institute, Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narottam Prasad","family":"Sahu","sequence":"additional","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asim Kumar","family":"Pal","sequence":"additional","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipesh","family":"Debnath","sequence":"additional","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"},{"name":"Regional Centre, ICAR-Central Inland Fisheries Research Institute, Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal Kant","family":"Jain","sequence":"additional","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3517-0184","authenticated-orcid":false,"given":"Rishikesh Subhashrao","family":"Dalvi","sequence":"additional","affiliation":[{"name":"Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India"},{"name":"Department of Zoology, Maharshi Dayanand College, Parel, Mumbai 400012, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0570-259X","authenticated-orcid":false,"given":"Petr","family":"Slama","sequence":"additional","affiliation":[{"name":"Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3622-9555","authenticated-orcid":false,"given":"Kavindra Kumar","family":"Kesari","sequence":"additional","affiliation":[{"name":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, 00076 Espoo, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4174-1852","authenticated-orcid":false,"given":"Shubhadeep","family":"Roychoudhury","sequence":"additional","affiliation":[{"name":"Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Preedy, V., and Patel, V. 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