{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:25:09Z","timestamp":1783466709866,"version":"3.55.0"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Lactic acid-producing bacteria (LAB) are being widely studied due to their probiotic potential. The aim of the present study was to determine and identify the presence of LAB from canine vaginal samples, as well as to evaluate their probiotic in vitro potential. Ninety-four bitches were included in the study. Vaginal samples were obtained by means of a sterile swab and streaked on Man Rogosa Sharpe agar plates. A total of 100 LAB strains were obtained and submitted to Gram stains and basic biochemical tests, which included catalase, oxidase and haemolysis tests. Thirteen strains belonging to the genera Lactobacillus (n = 10), Lactococcus (n = 2) and Pediococcus (n = 1) were selected as potential probiotics and further subjected to evaluation of resistance to gastrointestinal conditions (pH, lysozyme, bile salts and hydrogen peroxide) and safety and efficacy in vitro (resistance to antibiotics and antimicrobial capacity). Only three strains, one Lactobacillus lactis and two Lactobacillus plantarum, accomplished the requirements for being considered as potential in vitro probiotics.<\/jats:p>","DOI":"10.3390\/ani12060796","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T13:24:59Z","timestamp":1647869099000},"page":"796","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the Probiotic In Vitro Potential of Lactic Acid-Producing Bacteria from Canine Vagina: Possible Role in Vaginal Health"],"prefix":"10.3390","volume":"12","author":[{"given":"Brian","family":"Morales","sequence":"first","affiliation":[{"name":"Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4017-0421","authenticated-orcid":false,"given":"Livia","family":"Spadetto","sequence":"additional","affiliation":[{"name":"Toxicology Research Group, Faculty of Veterinary Medicine, University of Murcia, Campus Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria \u00c0ngels","family":"Calvo","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"Marc","family":"Yeste","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Griona, 17071 Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Arosemena","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2688-0275","authenticated-orcid":false,"given":"Teresa","family":"Rigau","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"},{"name":"Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9557-4888","authenticated-orcid":false,"given":"Maria Montserrat","family":"Rivera del Alamo","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"},{"name":"Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Faculty of Veterinary Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/1040-840291046759","article-title":"The lactic acid bacteria: A literature survey","volume":"28","author":"Carr","year":"2002","journal-title":"Crit. 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