{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:34:26Z","timestamp":1792154066718,"version":"4.4.0"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014434","name":"Polish National Agency for Academic Exchange","doi-asserted-by":"publisher","award":["PPI\/APM\/2019\/1\/00044\/U\/00001"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Dog semen freezing is gaining popularity, but it has to be performed in equipped facilities, which can be far from the place where the stud dog lives. The aim of this study was to evaluate whether freezing dog semen after 24 or 48 h of cooled transport to an equipped laboratory was possible when semen collection was performed in the field such as in local breeding kennels. Single ejaculates from different dogs (mixed breeds and ages) were collected. In Experiment I, 10 ejaculates were conventionally frozen using the Uppsala method or frozen after 24 or 48 h of storage in a Styrofoam transport box cooled by icepacks. In Experiment II, 10 ejaculates were used to assess the influence of two extenders (Uppsala chilling extender or freezing extender 1) used for semen dilution during the 24 or 48 h storage. Motility, morphology, membrane, and acrosome integrity were analyzed as well as spermatozoa zona-binding ability. No significant differences were observed among the frozen groups, regardless of freezing time (Experiment I) or extender (Experiment II). Motility at thawing, however, decreased in absolute value at 48 h. Freezing of freshly collected semen is the gold standard, but the results obtained in this study prompt the application of freezing after cooled transport for the long-term preservation of dog semen, especially if the transport can be organized in 24 h.<\/jats:p>","DOI":"10.3390\/ani12070816","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T12:20:25Z","timestamp":1648038025000},"page":"816","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Freezability of Dog Semen after Collection in Field Conditions and Cooled Transport"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1436-1985","authenticated-orcid":false,"given":"Martina","family":"Colombo","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Giorgia","family":"Morselli","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Franchi","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-7644","authenticated-orcid":false,"given":"Sabine","family":"Sch\u00e4fer-Somi","sequence":"additional","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9598-9098","authenticated-orcid":false,"given":"Gaia Cecilia","family":"Luvoni","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/S0195-5616(91)50054-1","article-title":"Achieving canine pregnancy by using frozen or chilled extended semen","volume":"21","year":"1991","journal-title":"Vet. 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