{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:58:35Z","timestamp":1792803515984,"version":"4.5.0"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005632","name":"NCBiR","doi-asserted-by":"publisher","award":["PBS3\/B8\/16\/2015"],"award-info":[{"award-number":["PBS3\/B8\/16\/2015"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences","award":["statutory research and development activity funds"],"award-info":[{"award-number":["statutory research and development activity funds"]}],"id":[{"id":"https:\/\/ror.org\/05cs8k179","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100014434","name":"Polish National Agency for Academic Exchange","doi-asserted-by":"publisher","award":["PPI\/APM\/2019\/1\/00044\/U\/00001"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Although artificial reproductive techniques (ART) are considered to be a valuable tool for species conservation, information about their introduction into clinical practice for wild felids is limited. The aim of this paper was to jointly describe cases of non-experimental sperm collection from males of various species of wild felids, performed by three European centers focused on feline reproduction. In total, the article presents 22 attempts of semen collection in 12 species of wild felids. The reasons for semen collection were: fertility assessment (10 cases), artificial insemination (5 cases), sperm rescue (postmortem collection for cryopreservation, 5 cases), and sperm banking (in vivo collection for cryopreservation, 2 cases). Semen collection was successful (defined as at least 1 \u00d7 106 spermatozoa) in 15 cases. The failures in obtaining spermatozoa were most probably due to (1) male infertility, (2) wrong age\/non-breeding season, or (3) recent multiple copulations. The cases presented here confirm that although ART have been introduced into clinical practice, they are mostly used in cases of infertility, not as routine breeding tools. Higher involvement of zoological gardens and private breeders is required, as many chances for preservation of valuable material are lost.<\/jats:p>","DOI":"10.3390\/ani12070871","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:22:14Z","timestamp":1648675334000},"page":"871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["How Can We Introduce ART into Wild Felid Conservation in Practice? Joint Experience in Semen Collection from Captive Wild Felids in Europe"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-8141","authenticated-orcid":false,"given":"Sylwia","family":"Prochowska","sequence":"first","affiliation":[{"name":"Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1056-0784","authenticated-orcid":false,"given":"Wojciech","family":"Ni\u017ca\u0144ski","sequence":"additional","affiliation":[{"name":"Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feline","family":"Snoeck","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eline","family":"Wydooghe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5010-6311","authenticated-orcid":false,"given":"Ann","family":"Van Soom","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Kochan","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krak\u00f3w, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasyl","family":"Stefanyk","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Biotechnology of Animal Reproduction Named after H.V.Zvereva, Stepan Gzhytskyi National University of Veterynary Medicine and Biotehnologies Lviv, 79010 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"ref_1","unstructured":"(2021, February 12). Version 2021-3. Available online: https:\/\/www.iucnredlist.org."},{"key":"ref_2","unstructured":"G\u0142owaci\u0144, Z. (2001). Felis silvestris Schreber, 1775. \u017bbik. Polish Red Data Book of Animals, Pa\u0144stwowe Wydawnictwo Rolnicze i Le\u015bne."},{"key":"ref_3","unstructured":"Von Schmalz-Peixoto, K.E. (2003). Factors Affecting Breeding in Captive Carnivora. [Ph.D. Thesis, University of Oxford]. Available online: http:\/\/www.carnivoreconservation.org\/files\/thesis\/schmalz_2003_phd.pdf."},{"key":"ref_4","first-page":"198","article-title":"Artificial insemination in the cat (Felis catus L.)","volume":"20","author":"Sojka","year":"1970","journal-title":"Lab. Anim. Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1530\/jrf.0.0520279","article-title":"Pregnancy in the domestic cat after artificial insemination with previously frozen spermatozoa","volume":"52","author":"Platz","year":"1978","journal-title":"J. Reprod. Fertil."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1530\/jrf.0.0230477","article-title":"Cat (Felis catus L.) spermatozoa require capacitation","volume":"23","author":"Hamner","year":"1970","journal-title":"J. Reprod. Fertil."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1136\/vr.108.13.282","article-title":"Successful induced ovulation and artificial insemination in the puma (Felis concolor)","volume":"108","author":"Moore","year":"1981","journal-title":"Vet. Rec."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1095\/biolreprod43.5.733","article-title":"In vitro fertilization and embryo development in vitro and in vivo in the tiger (Panthera tigris)","volume":"43","author":"Donoghue","year":"1990","journal-title":"Biol. Reprod."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Veraguas, D., Echeverry, D., Castro, F., and Rodriguez-Alvarez, L. (2017). Applied Biotechnologies in the Conservation of Wild Felids: In Vitro Embryo Production and Cellular Regenerative Therapies. Big Cats, IntechOpen.","DOI":"10.5772\/intechopen.71311"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0093-691X(86)90182-2","article-title":"Developing animal model systems for embryo technologies in rare and endangered wildlife","volume":"25","author":"Wildt","year":"1986","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0093-691X(99)00249-6","article-title":"Embryo technology in conservation efforts for endangered felids","volume":"53","author":"Pope","year":"2000","journal-title":"Theriogenology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.theriogenology.2006.03.024","article-title":"Application of assisted reproduction for population management in felids: The potential and reality for conservation of small cats","volume":"66","author":"Swanson","year":"2006","journal-title":"Theriogenology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0967199409990256","article-title":"Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction","volume":"18","author":"Cocchia","year":"2010","journal-title":"Zygote"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1089\/bio.2018.0104","article-title":"Semen Cryopreservation and Banking for the Conservation of Neotropical Carnivores","volume":"17","author":"Silva","year":"2019","journal-title":"Biopreserv. Biobank."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.theriogenology.2020.06.040","article-title":"Preservation of female genetic resources in feline species","volume":"156","author":"Jewgenow","year":"2020","journal-title":"Theriogenology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.theriogenology.2020.02.004","article-title":"Reproductive biology and biotechnologies in wild felids","volume":"150","author":"Thongphakdee","year":"2020","journal-title":"Theriogenology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Crosier, A.E., Lamy, J., Bapodra, P., Rapp, S., Maly, M., Junge, R., Haefele, H., Ahistus, J., Santiestevan, J., and Comizzoli, P. (2020). First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer. Animals, 10.","DOI":"10.3390\/ani10101811"},{"key":"ref_18","first-page":"15","article-title":"Felid-Gamete-Rescue Within EAZA\u2014Efforts and Results in Biobanking Felid Oocytes and Sperm","volume":"7","author":"Jewgenow","year":"2019","journal-title":"JZAR"},{"key":"ref_19","first-page":"93","article-title":"Reproductive Biology and a Genome Resource Bank of Felidae","volume":"48","author":"Amstislavsky","year":"2017","journal-title":"Ontogenez"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2478\/jvetres-2019-0043","article-title":"ARTs in Wild Felid Conservation Programmes in Poland and in the World","volume":"63","author":"Kochan","year":"2019","journal-title":"J. Vet. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.anireprosci.2008.05.070","article-title":"Developing biological resource banks as a supporting tool for wildlife reproduction and conservation The Iberian lynx bank as a model for other endangered species","volume":"112","author":"Simon","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.anireprosci.2004.05.008","article-title":"International training programs in reproductive sciences for conservation of Latin American felids","volume":"82\u201383","author":"Swanson","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2017.12.019","article-title":"Comparison of semen samples collected from wild and captive jaguars (Panthera onca) by urethral catheterization after pharmacological induction","volume":"195","author":"Araujo","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1095\/biolreprod38.2.245","article-title":"A comparative analysis of ejaculate and hormonal characteristics of the captive male cheetah, tiger, leopard, and puma","volume":"38","author":"Wildt","year":"1988","journal-title":"Biol. Reprod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1530\/jrf.0.1020229","article-title":"Seasonal effects on seminal and endocrine traits in the captive snow leopard (Panthera uncia)","volume":"102","author":"Johnston","year":"1994","journal-title":"J. Reprod. Fertil."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S0011-2240(02)00035-4","article-title":"Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: Influence of adding and removing cryoprotectant","volume":"44","author":"Pukazhenthi","year":"2002","journal-title":"Cryobiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ygcen.2009.06.021","article-title":"Fecal endocrine profiles and ejaculate traits in black-footed cats (Felis nigripes) and sand cats (Felis margarita)","volume":"165","author":"Herrick","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_28","first-page":"173","article-title":"Ocena wybranych w\u0142a\u015bciwo\u015bci plemnik\u00f3w naj\u0105drzowych kota domowego i ich konserwacja w niskich temperaturach","volume":"61","author":"Dejneka","year":"2005","journal-title":"Med. Weter"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.anireprosci.2003.11.003","article-title":"The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa","volume":"84","author":"Hermansson","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.theriogenology.2016.09.049","article-title":"Reproductive analysis of male and female captive jaguars (Panthera onca) in a Colombian zoological park","volume":"89","author":"Howard","year":"2017","journal-title":"Theriogenology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1016\/j.theriogenology.2006.01.017","article-title":"Ovarian response to repeated administration of alternating exogenous gonadotropin regimens in the ocelot (Leopardus pardalis) and tigrinus (Leopardus tigrinus)","volume":"66","author":"Dias","year":"2006","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1530\/jrf.0.1070053","article-title":"Timing of ovulation after gonadotrophin induction and its importance to successful intrauterine insemination in the tiger (Panthera tigris)","volume":"107","author":"Donoghue","year":"1996","journal-title":"J. Reprod. Fertil."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2981\/0909-6396(2006)12[109:ROCMIL]2.0.CO;2","article-title":"Risk of capture-related mortality in large free-ranging mammals: Experiences from Scandinavia","volume":"12","author":"Arnemo","year":"2006","journal-title":"Wildl. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.theriogenology.2005.05.032","article-title":"Welfare aspects of theriogenology: Investigating alternatives to electroejaculation of bulls","volume":"64","author":"Palmer","year":"2005","journal-title":"Theriogenology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.theriogenology.2007.10.019","article-title":"Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration","volume":"69","author":"Zambelli","year":"2008","journal-title":"Theriogenology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.theriogenology.2012.02.026","article-title":"Improved semen collection method for wild felids: Urethral catheterization yields high sperm quality in African lions (Panthera leo)","volume":"78","author":"Lueders","year":"2012","journal-title":"Theriogenology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1638\/2013-0269R.1","article-title":"Successful nonsurgical artificial insemination and hormonal monitoring in an Asiatic golden cat (Catopuma temmincki)","volume":"45","author":"Lueders","year":"2014","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.theriogenology.2016.12.034","article-title":"Sperm evaluation of Jungle Cat (Felis chaus) obtained by urethral catheterization (CT) after medetomidine administration","volume":"91","author":"Kheirkhah","year":"2017","journal-title":"Theriogenology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1111\/rda.12626","article-title":"Influence of Different Protocols of Urethral Catheterization after Pharmacological Induction (Ur.Ca.P.I.) on Semen Quality in the Domestic Cat","volume":"50","author":"Cunto","year":"2015","journal-title":"Reprod. Domest. Anim."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1071\/RDv31n1Ab101","article-title":"101 Assessment of semen traits in servals (Leptailurus serval) and Canada lynx (Lynx canadensis)","volume":"31","author":"Gonzalez","year":"2019","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1365-2605.1991.tb01071.x","article-title":"Variation of semen quality in normal men","volume":"14","author":"Mallidis","year":"1991","journal-title":"Int. J. Androl."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/rda.12074","article-title":"Spermatogenic function in cats","volume":"47","author":"Tsutsui","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1071\/RD18350","article-title":"Practical application of laparoscopic oviductal artificial insemination for the propagation of domestic cats and wild felids","volume":"31","author":"Swanson","year":"2018","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.theriogenology.2019.07.022","article-title":"Non-surgical artificial insemination using a GnRH analogue for ovulation induction during natural oestrus in African lions (Panthera leo)","volume":"139","author":"Callealta","year":"2019","journal-title":"Theriogenology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1292\/jvms.09-0260","article-title":"The qualities of cryopreserved epididymal sperm collected from feline epididymides stored at low temperature","volume":"72","author":"Toyonaga","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.theriogenology.2009.07.023","article-title":"Effect of storage of domestic cat (Felis catus) epididymides at 5 degrees C on sperm quality and cryopreservation","volume":"72","author":"Gomendio","year":"2009","journal-title":"Theriogenology"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/7\/871\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:46:32Z","timestamp":1760136392000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/7\/871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,30]]},"references-count":46,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["ani12070871"],"URL":"https:\/\/doi.org\/10.3390\/ani12070871","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,30]]}}}