{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:54:14Z","timestamp":1789606454581,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish National Agency for Academic Exchange (NAWA)","award":["PPI\/APM\/2019\/1\/00044\/U\/00001'"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001'"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>To evaluate the expression of AMH and its receptor AMHRII, ovaries of 33 p cats were investigated by western blot and immunohistochemistry. After ovariohysterectomy, the cats were grouped according to pregnancy stages and ovarian\/placental endocrine activity: group I (n = 3, 24\u201329 days), II (n = 8, 32\u201340 days), III (n = 4, 41\u201346 days), IV (n = 6, 53\u201361 days) and according to cycle stages: V (n = 6, interestrus) and VI (n = 6, estrus). Serum progesterone- and AMH-concentration was measured. Follicle numbers did not differ between groups. The number of corpora lutea was higher in pregnant cats than in the non-pregnant cats. Serum AMH concentration was at maximum between day 30 and 50 of gestation, and was higher than in non-pregnant cats, then decreased towards term (p &lt; 0.05). In the ovaries, AMH immunopositivity was observed in granulosa cells of secondary and antral follicles, and in interstitial cells of corpora lutea; highest percentage of immunopositive areas was detected in group III (p &lt; 0.05). A positive correlation between the number of corpora lutea and the positive AMH signals in ovarian tissue was determined (r2 = 0.832, p &lt; 0.05); however, only during mid-gestation (group II). Expression of AMHRII was in close co-localization with AMH and strong in the interstitial cells surrounding follicles undergoing atresia. AMHRII expression did not differ between pregnant groups but was higher compared to estrus cats (p \u02c2 0.05). We conclude that AMH and AMHRII expression in the feline ovary is comparable to other species. The high serum AMH concentration and ovarian AMHRII expression between day 30 and 50 of gestation are probably related to ovarian activity and follicular atresia.<\/jats:p>","DOI":"10.3390\/ani12070877","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:22:14Z","timestamp":1648675334000},"page":"877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Expression of Anti-M\u00fcllerian Hormone and Its Type 2 Receptor in the Ovary of Pregnant and Cyclic Domestic Cats"],"prefix":"10.3390","volume":"12","author":[{"given":"Nilg\u00fcn","family":"G\u00fcltiken","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0636-4214","authenticated-orcid":false,"given":"Murat","family":"Yarim","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selim","family":"Aslan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Near East University, Nicosia 99138, Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hande","family":"G\u00fcrler","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4050-429X","authenticated-orcid":false,"given":"Gul Fatma","family":"Yarim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00fcge","family":"Tuncay","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2552-5159","authenticated-orcid":false,"given":"Sinem","family":"\u0130nal","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Ondokuz Mayis, Samsun 55200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-7644","authenticated-orcid":false,"given":"Sabine","family":"Sch\u00e4fer-Somi","sequence":"additional","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/0092-8674(86)90783-X","article-title":"Isolation of the bovine and human genes for m\u00fcllerian inhibiting substance and expression of the human gene in animal cells","volume":"45","author":"Cate","year":"1986","journal-title":"Cell"},{"key":"ref_2","first-page":"379","article-title":"Problems of fetal endocrinology: The gonadal and hypophyseal hormones","volume":"8","author":"Jost","year":"1953","journal-title":"Recent Prog. Horm. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1210\/clinem\/dgaa513","article-title":"Anti-M\u00fcllerian Hormone and Ovarian Reserve: Update on Assessing Ovarian Function","volume":"105","author":"Moolhuijsen","year":"2020","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_4","first-page":"548","article-title":"The Testicular Hormones AMH, InhB, INSL3, and Testosterone Can Be Independently Deficient in Older Men","volume":"72","author":"Chong","year":"2017","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"109","DOI":"10.4103\/aja.aja_83_18","article-title":"Regulation of anti-M\u00fcllerian hormone (AMH) in males and the associations of serum AMH with the disorders of male fertility","volume":"21","author":"Xu","year":"2019","journal-title":"Asian J. Androl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.theriogenology.2018.05.012","article-title":"Anti-M\u00fcllerian hormone profiling in prepubertal horses and its relationship with gonadal function","volume":"117","author":"Scarlet","year":"2018","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1530\/REP-17-0104","article-title":"Anti-M\u00fcllerian Hormone (AMH) and fertility management in agricultural species","volume":"154","author":"Mossa","year":"2017","journal-title":"Reproduction"},{"key":"ref_8","first-page":"55","article-title":"Characterization of ovarian aging and reproductive senescence in vervet monkeys (Chlorocebus aethiops sabaeus)","volume":"64","author":"Atkins","year":"2014","journal-title":"Comp. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Umer, S., Sammad, A., Zou, H., Khan, A., Weldegebriall Sahlu, B., Hao, H., Zhao, X., Wang, Y., Zhao, S., and Zhu, H. (2019). Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones. Genes, 10.","DOI":"10.3390\/genes10121038"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.theriogenology.2014.11.016","article-title":"Concentrations of anti-M\u00fcllerian hormone in the domestic cat. Relation with spay or neuter status and serum estradiol","volume":"83","year":"2015","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1210\/endo.143.3.8691","article-title":"Anti-M\u00fcllerian hormone inhibits initiation of primordial follicle growth in the mouse ovary","volume":"143","author":"Durlinger","year":"2002","journal-title":"Endocrinology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1530\/rep.0.1240601","article-title":"Regulation of ovarian function: The role of anti-M\u00fcllerian hormone","volume":"124","author":"Durlinger","year":"2002","journal-title":"Reproduction"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1210\/jc.2014-3336","article-title":"Circulating AMH reflects ovarian morphology by magnetic resonance imaging and 3D ultrasound in 121 healthy girls","volume":"100","author":"Hagen","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1210\/jc.2006-1697","article-title":"Stage-specific expression of androgen receptor, follicle-stimulating hormone receptor, and anti-M\u00fcllerian hormone type II receptor in single, isolated, human preantral follicles: Relevance to polycystic ovaries","volume":"92","author":"Rice","year":"2007","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1093\/humupd\/dmw027","article-title":"Interactions between androgens, FSH, anti-M\u00fcllerian hormone and estradiol during folliculogenesis in the human normal and polycystic ovary","volume":"22","author":"Dewailly","year":"2016","journal-title":"Hum. Reprod. Update"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1093\/humrep\/des460","article-title":"Loss of LH-induced down-regulation of anti-M\u00fcllerian hormone receptor expression may contribute to anovulation in women with polycystic ovary syndrome","volume":"28","author":"Pierre","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1093\/humrep\/det446","article-title":"Anti-Mullerian hormone is a more accurate predictor of individual time to menopause than mother\u2019s age at menopause","volume":"29","author":"Depmann","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1007\/s10815-014-0324-y","article-title":"Anti-m\u00fcllerian hormone levels as a predictor of the pregnancy rate in women of advanced reproductive age","volume":"31","author":"Sahmay","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/rda.12822","article-title":"Determination of the normal reference interval for anti-M\u00fcllerian hormone (AMH) in bitches and use of AMH as a potential predictor of litter size","volume":"52","author":"Hollinshead","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/rda.12827","article-title":"Age and anti-M\u00fcllerian hormone levels predict the success of in vitro maturation of cat oocytes","volume":"52","author":"Snoeck","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.fertnstert.2015.04.044","article-title":"Changes in antim\u00fcllerian hormone levels in early pregnancy are associated with preterm birth","volume":"104","author":"Stegmann","year":"2015","journal-title":"Fertil. Steril."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.rbmo.2017.02.005","article-title":"Anti-M\u00fcllerian hormone kinetics in pregnancy and post-partum: A systematic review","volume":"34","author":"McCredie","year":"2017","journal-title":"Reprod. Biomed. Online"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Pankhurst, M.W., Clark, C.A., Zarek, J., Laskin, C.A., and McLennan, I.S. (2016). Changes in Circulating ProAMH and Total AMH during Healthy Pregnancy and Post-Partum: A Longitudinal Study. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0162509"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.cvsm.2018.02.008","article-title":"Gestational Aging and Determination of Parturition Date in the Bitch and Queen Using Ultrasonography and Radiography","volume":"48","author":"Lopate","year":"2018","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1053\/jfms.2001.0153","article-title":"Ultrasound aspects of fetal and extrafetal structures in pregnant cats","volume":"4","author":"Zambelli","year":"2002","journal-title":"J. Feline Med. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1016\/j.theriogenology.2004.02.009","article-title":"Correlation between fetal age and ultrasonographic measurements during the second half of pregnancy in domestic cats (Felis catus)","volume":"62","author":"Zambelli","year":"2004","journal-title":"Theriogenology"},{"key":"ref_27","first-page":"165","article-title":"Regulation of progesterone during pregnancy in the cat: Studies on the roles of corpora lutea, placenta and prolactin secretion","volume":"47","author":"Verstegen","year":"1993","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jsbmb.2012.07.005","article-title":"Progesterone and estradiol in cat placenta--biosynthesis and tissue concentration","volume":"132","author":"Braun","year":"2012","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"364787","DOI":"10.1155\/2014\/364787","article-title":"Placental origin of prostaglandin F2\u03b1 in the domestic cat","volume":"2014","author":"Siemieniuch","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/j.fertnstert.2009.07.1676","article-title":"Longitudinal assessment of antim\u00fcllerian hormone during pregnancy-relationship with maternal adiposity, insulin, and adiponectin","volume":"93","author":"Nelson","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s10815-010-9433-4","article-title":"Maternal antimullerian hormone levels do not predict fetal aneuploidy","volume":"27","author":"Plante","year":"2010","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1477-7827-11-60","article-title":"Anti-Mullerian-hormone levels during pregnancy and postpartum","volume":"11","author":"Kauth","year":"2013","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1093\/humrep\/deh819","article-title":"Anti-M\u00fcllerian hormone concentrations in maternal serum during pregnancy","volume":"20","author":"Giulini","year":"2005","journal-title":"Hum. Reprod."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lahoz, B., Alabart, J.L., Monniaux, D., Mermillod, P., and Folch, J. (2012). Anti-M\u00fcllerian hormone plasma concentration in prepubertal ewe lambs as a predictor of their fertility at a young age. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-118"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1210\/jc.2006-2425","article-title":"Serum anti-mullerian hormone concentrations are not altered by acute administration of follicle stimulating hormone in polycystic ovary syndrome and normal women","volume":"92","author":"Wachs","year":"2007","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"77.e1","DOI":"10.1016\/j.ajog.2006.07.046","article-title":"M\u00fcllerian inhibiting substance and disrupted folliculogenesis in polycystic ovary syndrome","volume":"196","author":"Wang","year":"2007","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1071\/RD17377","article-title":"Anti-M\u00fcllerian hormone receptor type 2 is expressed in gonadotrophs of postpubertal heifers to control gonadotrophin secretion","volume":"30","author":"Kereilwe","year":"2018","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1439-0531.2009.01452.x","article-title":"The role of the ovary for the maintenance of pregnancy in cats","volume":"44","author":"Tsutsui","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"10055","DOI":"10.1038\/ncomms10055","article-title":"Novel role for anti-M\u00fcllerian hormone in the regulation of GnRH neuron excitability and hormone secretion","volume":"7","author":"Cimino","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1095\/biolreprod25.1.15","article-title":"Ovarian activity, circulating hormones, and sexual behavior in the cat. I. Relationships during the coitus-induced luteal phase and the estrous period without mating","volume":"25","author":"Wildt","year":"1981","journal-title":"Biol. Reprod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4951","DOI":"10.1210\/endo.136.11.7588229","article-title":"Anti-m\u00fcllerian hormone and anti-m\u00fcllerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle, and gonadotropin-induced follicle growth","volume":"136","author":"Baarends","year":"1995","journal-title":"Endocrinology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1093\/biolre\/ioy140","article-title":"Anti-M\u00fcllerian hormone type II receptor in avian follicle development","volume":"99","author":"Lemcke","year":"2018","journal-title":"Biol. Reprod."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3376","DOI":"10.1210\/jc.2018-00549","article-title":"Expression of M\u00fcllerian-Inhibiting Substance\/Anti-M\u00fcllerian Hormone Type II Receptor in the Human Theca Cells","volume":"103","author":"Cheon","year":"2018","journal-title":"J. Clin. Endocrinol. Metab."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/7\/877\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:46:51Z","timestamp":1760136411000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/7\/877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,30]]},"references-count":43,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["ani12070877"],"URL":"https:\/\/doi.org\/10.3390\/ani12070877","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,30]]}}}