{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:22:36Z","timestamp":1790281356091,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T00:00:00Z","timestamp":1651449600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Program for Research Groups of Excellence of the Seneca Foundation, Murcia, Spain","doi-asserted-by":"publisher","award":["19894\/GERM\/15"],"award-info":[{"award-number":["19894\/GERM\/15"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Program for Research Groups of Excellence of the Seneca Foundation, Murcia, Spain","doi-asserted-by":"publisher","award":["KK.01.1.16.0004"],"award-info":[{"award-number":["KK.01.1.16.0004"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Structural and Investment Funds","award":["19894\/GERM\/15"],"award-info":[{"award-number":["19894\/GERM\/15"]}]},{"name":"European Structural and Investment Funds","award":["KK.01.1.16.0004"],"award-info":[{"award-number":["KK.01.1.16.0004"]}]},{"name":"University of Murcia through a postdoctoral grant (Margarita Salas)","award":["19894\/GERM\/15"],"award-info":[{"award-number":["19894\/GERM\/15"]}]},{"name":"University of Murcia through a postdoctoral grant (Margarita Salas)","award":["KK.01.1.16.0004"],"award-info":[{"award-number":["KK.01.1.16.0004"]}]},{"name":"European Union Next Generation","award":["19894\/GERM\/15"],"award-info":[{"award-number":["19894\/GERM\/15"]}]},{"name":"European Union Next Generation","award":["KK.01.1.16.0004"],"award-info":[{"award-number":["KK.01.1.16.0004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Changes in the salivary proteome in 12 horses with the two diseases included in equine gastric ulcer syndrome (EGUS), equine glandular gastric disease (EGGD) (n = 6) and equine squamous gastric disease (ESGD) (n = 6), were evaluated using a high-resolution LC-MS\/MS analysis of TMT-labelled peptides and compared to 10 healthy control horses. Serum was also analysed for comparative purposes. The comparison between the horses with EGGD and controls showed significant changes in 10 salivary proteins, whereas 36 salivary proteins were differently abundant between ESGD and control groups. The most upregulated proteins in the case of EGGD were related to immune activation whereas, in horses with ESGD, the most significantly changed proteins were associated with squamous cell regulation and growth. Compared to serum, saliva showed a higher number of proteins with significant changes and a different pattern of changes. The proteins identified in our study, in addition to providing new information about the pathophysiological mechanisms in these diseases, could have the potential to be novel biomarkers for the diagnosis or monitoring of EGGD and ESGD.<\/jats:p>","DOI":"10.3390\/ani12091169","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T08:26:35Z","timestamp":1651566395000},"page":"1169","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6865-8712","authenticated-orcid":false,"given":"Alberto","family":"Mu\u00f1oz-Prieto","sequence":"first","affiliation":[{"name":"Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"},{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2898-0410","authenticated-orcid":false,"given":"Maria Dolores","family":"Contreras-Aguilar","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Joaqu\u00edn","family":"Cer\u00f3n","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0335-482X","authenticated-orcid":false,"given":"Ignacio","family":"Ayala","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Martin-Cuervo","sequence":"additional","affiliation":[{"name":"Medicine Animal, Faculty of Veterinary Medicine of C\u00e1ceres, University of Extremadura, Avenida de la Universidad S-N, 10002 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Gonzalez-Sanchez","sequence":"additional","affiliation":[{"name":"BioQuant, Faculty of Biosciences, Heidelberg University, Im Neuenheimer Feld 267, 69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-4576","authenticated-orcid":false,"given":"Stine","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josipa","family":"Kule\u0161","sequence":"additional","affiliation":[{"name":"Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8975-9600","authenticated-orcid":false,"given":"An\u0111elo","family":"Beleti\u0107","sequence":"additional","affiliation":[{"name":"Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Rubi\u0107","sequence":"additional","affiliation":[{"name":"Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Mrljak","sequence":"additional","affiliation":[{"name":"Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Tecles","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanni","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1111\/jvim.13578","article-title":"European College of Equine Internal Medicine Consensus 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