{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T02:55:52Z","timestamp":1762052152537,"version":"build-2065373602"},"reference-count":19,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,15]],"date-time":"2022-05-15T00:00:00Z","timestamp":1652572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Projects of Innovative Research Groups of the Natural Science Foundation of Hubei Province","award":["2019CFA015","2022J05","201802"],"award-info":[{"award-number":["2019CFA015","2022J05","201802"]}]},{"name":"Scientific Research Project of Wuhan Polytechnic University","award":["2019CFA015","2022J05","201802"],"award-info":[{"award-number":["2019CFA015","2022J05","201802"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["2019CFA015","2022J05","201802"],"award-info":[{"award-number":["2019CFA015","2022J05","201802"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of this study was to compare two supplementary doses (6000 vs. 12,000 IU\/kg) of vitamin A (VA) on the performance, development of intestine and immune organs, as well as gene expression of inflammatory factors in young Hy-Line Brown laying pullets. A total of 288 one-day-old Hy-Line Brown laying pullets (weighing 42.15 \u00b1 0.23 g) were allotted into two treatments with 12 replicate cages and 12 birds per cage. During the 35-day period, the pullets were fed a basal diet supplemented with different doses of VA (6000 IU\/kg VA in control group; 12,000 IU\/kg VA in treatment group), respectively. The results showed that supplementary high doses of VA reduced the feed-to-gain ratio from day 21 to 35 (p &lt; 0.05). Moreover, the pullets fed high doses of VA diets had increased length and relative weight of duodenum, jejunum, and ileum (p &lt; 0.05). From observations on morphology, high doses of VA diets increased the villus height and the ratio of villus height to crypt depth in the jejunum and ileum (p &lt; 0.05). High doses of VA diets also increased the relative weight of immune organs (p &lt; 0.05). Furthermore, the gene expressions of inflammatory factors were decreased in the thymus of the pullets fed high doses of VA diets (p &lt; 0.05). In summary, supplementary 12,000 IU\/kg doses of VA improved performance and intestine and immune organ development, and alleviated gene expressions of inflammatory factors in young Hy-Line Brown laying pullets.<\/jats:p>","DOI":"10.3390\/ani12101271","type":"journal-article","created":{"date-parts":[[2022,5,15]],"date-time":"2022-05-15T09:48:22Z","timestamp":1652608102000},"page":"1271","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Comparison of Two Supplementary Doses of Vitamin A on Performance, Intestine and Immune Organ Development, as well as Gene Expression of Inflammatory Factors in Young Hy-Line Brown Laying Pullets"],"prefix":"10.3390","volume":"12","author":[{"given":"Qinliang","family":"Chen","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqing","family":"Han","sequence":"additional","affiliation":[{"name":"Wuhan Hualuo Branch, China Animal Husbandry Industry Co., Ltd., Wuhan 430050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulan","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,15]]},"reference":[{"key":"ref_1","first-page":"379","article-title":"Influence of xylanase and vitamin A in wheat-based diet on performance, nutrients digestibility, small intestinal morpholgy and digesta viscosity in broiler chickens","volume":"36","author":"Khoramabadi","year":"2014","journal-title":"Acta Sci."},{"key":"ref_2","first-page":"65","article-title":"Dietary vitamin A supplementary effects on performance and immuno-competence of broiler chickens","volume":"20","author":"Faluyi","year":"2017","journal-title":"Arch. 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