{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T02:55:25Z","timestamp":1780973725387,"version":"3.54.1"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Open Project of Key Laboratory of SFGA on Conservation Biology of Rare Animals in the Giant Panda National Park","award":["KLSFGAGP2020.003"],"award-info":[{"award-number":["KLSFGAGP2020.003"]}]},{"name":"the Open Project of Key Laboratory of SFGA on Conservation Biology of Rare Animals in the Giant Panda National Park","award":["2020NTD04"],"award-info":[{"award-number":["2020NTD04"]}]},{"name":"the Open Project of Key Laboratory of SFGA on Conservation Biology of Rare Animals in the Giant Panda National Park","award":["16011211062"],"award-info":[{"award-number":["16011211062"]}]},{"name":"Fundamental Research Funds for the Central Universities, Southwest Minzu University","award":["KLSFGAGP2020.003"],"award-info":[{"award-number":["KLSFGAGP2020.003"]}]},{"name":"Fundamental Research Funds for the Central Universities, Southwest Minzu University","award":["2020NTD04"],"award-info":[{"award-number":["2020NTD04"]}]},{"name":"Fundamental Research Funds for the Central Universities, Southwest Minzu University","award":["16011211062"],"award-info":[{"award-number":["16011211062"]}]},{"name":"High-level Talent Introduction Programs of Southwest Minzu University","award":["KLSFGAGP2020.003"],"award-info":[{"award-number":["KLSFGAGP2020.003"]}]},{"name":"High-level Talent Introduction Programs of Southwest Minzu University","award":["2020NTD04"],"award-info":[{"award-number":["2020NTD04"]}]},{"name":"High-level Talent Introduction Programs of Southwest Minzu University","award":["16011211062"],"award-info":[{"award-number":["16011211062"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>As an assisted breeding technique, artificial insemination has become the main effective practical approach in the captive breeding programs of giant panda worldwide. The composition of seminal plasma plays an important role in the success of breeding. The present work is the first attempt to characterize, by proton magnetic resonance spectroscopy (1H-NMR), the metabolome of healthy giant panda seminal plasma. A total of 35 molecules were quantified, with the concentration of 2,3-butanediol being significantly different between individuals younger than 8 years and older than 13 years, and other distinct age-related trends were highlighted by a multivariate analysis. Isopropanol\u2019s concentration was significantly linked to estrus stages. Besides, the variations in the metabolome\u2019s profile during storage were also evaluated. This study may serve as a reference for further research wishing to shed light on the biological mechanisms affecting giant panda sperm\u2019s overall quality and may ultimately lead to novel approaches to giant panda artificial insemination.<\/jats:p>","DOI":"10.3390\/ani12121536","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T23:50:14Z","timestamp":1655250614000},"page":"1536","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by 1H-NMR Spectroscopy"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3486-1936","authenticated-orcid":false,"given":"Chenglin","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Dong","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiwu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, The China Conservation and Research Center for the Giant Panda, Dujiangyan 611800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5635-8277","authenticated-orcid":false,"given":"Likou","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1593-7376","authenticated-orcid":false,"given":"Luca","family":"Laghi","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,14]]},"reference":[{"key":"ref_1","unstructured":"Swaisgood, R., Wang, D., and Wei, F. (2022, March 10). The IUCN Red List of Threatened Species. Available online: https:\/\/www.iucnredlist.org\/species\/712\/121745669."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.5686\/jjzwm.2.107","article-title":"Physiological Characteristics of Electro-Ejaculated Giant Panda (Ailuropoda melanoleuca) Semen","volume":"2","author":"Feng","year":"1997","journal-title":"Jpn. J. Zoo Wildl. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1016\/j.theriogenology.2006.01.014","article-title":"Semen Quality in a Giant Panda (Ailuropoda melanoleuca) in Relation to Estrus of a Nearby Resident Female Panda","volume":"66","author":"Tsutsui","year":"2006","journal-title":"Theriogenology"},{"key":"ref_4","first-page":"73","article-title":"A Note on the Artificial Insemination of Giant Panda","volume":"2","author":"Weixin","year":"1981","journal-title":"Chin. J. Anim. Vet. Sci."},{"key":"ref_5","unstructured":"Huang, Y., Wang, P., Zhang, G., Zhang, H., Li, D., Du, J., Wei, R., Tang, C., Spindler, R.E., and Wildt, D.E. (2002, January 27\u201328). Use of Artificial Insemination to Enhance Propagation of Giant Pandas at the Wolong Breeding Center. Proceedings of the International Symposium on Assisted Reproductive Technologies (ART) for Conservation and Genetic Management of Wildlife, Omaha, NE, USA."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/zoo.1430020309","article-title":"The Breeding of Captive Wild Species by Artificial Methods","volume":"2","author":"Seager","year":"1983","journal-title":"Zoo Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1530\/jrf.0.0670009","article-title":"Electroejaculation and Semen Analysis and Freezing in the Giant Panda (Ailuropoda melanoleuca)","volume":"67","author":"Platz","year":"1983","journal-title":"J. Reprod. Fertil."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/zoo.1430130111","article-title":"Semen Evaluation of Giant Pandas (Ailuropoda melanoleuca) at the Wolong Reserve","volume":"13","author":"Chen","year":"1994","journal-title":"Zoo Biol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Menezes, E.B., Velho, A.L.C., Santos, F., Dinh, T., Kaya, A., Topper, E., Moura, A.A., and Memili, E. (2019). Uncovering Sperm Metabolome to Discover Biomarkers for Bull Fertility. BMC Genom., 20.","DOI":"10.1186\/s12864-019-6074-6"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Cazaux Velho, A.L., Menezes, E., Dinh, T., Kaya, A., Topper, E., Moura, A.A., and Memili, E. (2018). Metabolomic Markers of Fertility in Bull Seminal Plasma. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0195279"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/jes.2010.6","article-title":"Parabens in Urine, Serum and Seminal Plasma from Healthy Danish Men Determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS\/MS)","volume":"21","author":"Frederiksen","year":"2011","journal-title":"J. Expo. Sci. Environ. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1007\/s10815-014-0282-4","article-title":"Identification of Biochemical Differences between Different Forms of Male Infertility by Nuclear Magnetic Resonance (NMR) Spectroscopy","volume":"31","author":"Jayaraman","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/andr.12707","article-title":"Metabolomics Analysis of Seminal Plasma in Patients with Idiopathic Oligoasthenoteratozoospermia Using High-Resolution NMR Spectroscopy","volume":"8","author":"Mumcu","year":"2020","journal-title":"Andrology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"103349","DOI":"10.1016\/j.jri.2021.103349","article-title":"Identification of Distinct Seminal Plasma Cytokine Profiles Associated with Male Age and Lifestyle Characteristics in Unexplained Recurrent Pregnancy Loss","volume":"147","author":"Lashley","year":"2021","journal-title":"J. Reprod. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1017\/S1751731117001811","article-title":"Age-Related Differences of Semen Quality, Seminal Plasma, and Spermatozoa Antioxidative and Oxidative Stress Variables in Bulls during Cold and Warm Periods of the Year","volume":"12","author":"Vince","year":"2018","journal-title":"Animal"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.apjr.2016.07.006","article-title":"Comparative Blood and Seminal Plasma Oxidant\/Antioxidant Status of Arab Stallions with Different Ages and Their Relation to Semen Quality","volume":"5","author":"Rawash","year":"2016","journal-title":"Asian Pac. J. Reprod."},{"key":"ref_17","first-page":"644","article-title":"Reproductive Development of Santa In\u00eas Rams during the First Year of Life: Body and Testis Growth, Testosterone Concentrations, Sperm Parameters, Age at Puberty and Seminal Plasma Proteins","volume":"45","author":"Souza","year":"2010","journal-title":"Reprod. Domest. Anim."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0378-4320(93)90095-9","article-title":"Influence of Age and Season on Certain Biochemical Constituents of Seminal Plasma of Arabian Horses","volume":"32","author":"Ismail","year":"1993","journal-title":"Anim. Reprod. Sci."},{"key":"ref_19","first-page":"195","article-title":"Protracted Reproductive Seasonality in the Male Giant Panda (Ailuropoda melanoleuca) Reflected by Patterns in Androgen Profiles, Ejaculate Characteristics, and Selected Behaviors","volume":"86","author":"Hou","year":"2012","journal-title":"Biol. Reprod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.anireprosci.2008.04.013","article-title":"Components of Stallion Seminal Plasma and the Effects of Seminal Plasma on Sperm Longevity","volume":"107","author":"Kareskoski","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pavaneli, A.P.P., Recuero, S., Chaves, B.R., Garcia-Bonavila, E., Llavanera, M., Pinart, E., Bonet, S., de Andrade, A.F.C., and Yeste, M. (2020). The Presence of Seminal Plasma during Liquid Storage of Pig Spermatozoa at 17 \u00b0C Modulates Their Ability to Elicit In Vitro Capacitation and Trigger Acrosomal Exocytosis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21124520"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.trac.2014.04.009","article-title":"Nuclear Magnetic Resonance for Foodomics beyond Food Analysis","volume":"59","author":"Laghi","year":"2014","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhu, C., Li, C., Wang, Y., and Laghi, L. (2019). Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy. Metabolites, 9.","DOI":"10.3390\/metabo9030041"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1007\/s11306-018-1403-3","article-title":"Characterization of Trotter Horses Urine Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy","volume":"14","author":"Zhu","year":"2018","journal-title":"Metabolomics"},{"key":"ref_25","unstructured":"R Development Core Team (2007). Computational Many-Particle Physics, Springer."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0168-583X(95)00908-6","article-title":"Algorithm for Fitting XRF, SEM and PIXE X-Ray Spectra Backgrounds","volume":"109\u2013110","author":"Kneen","year":"1996","journal-title":"Nucl. Instrum. Methods Phys. Res. Sect. B Beam Interact. Mater. At."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1366\/000370210792434350","article-title":"Optimal Choice of Baseline Correction for Multivariate Calibration of Spectra","volume":"64","author":"Liland","year":"2010","journal-title":"Appl. Spectrosc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4281","DOI":"10.1021\/ac051632c","article-title":"Probabilistic Quotient Normalization as Robust Method to Account for Dilution of Complex Biological Mixtures. Application in 1H NMR Metabonomics","volume":"78","author":"Dieterle","year":"2006","journal-title":"Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.2517-6161.1964.tb00553.x","article-title":"An Analysis of Transformations","volume":"26","author":"Box","year":"1964","journal-title":"J. R. Stat. Soc. Ser. B Methodol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1198\/004017004000000563","article-title":"ROBPCA: A New Approach to Robust Principal Component Analysis","volume":"47","author":"Hubert","year":"2005","journal-title":"Technometrics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1021\/acs.jproteome.9b00564","article-title":"First Steps toward the Giant Panda Metabolome Database: Untargeted Metabolomics of Feces, Urine, Serum, and Saliva by 1H NMR","volume":"19","author":"Zhu","year":"2020","journal-title":"J. Proteome Res."},{"key":"ref_32","first-page":"243","article-title":"Studies on the Reproduction of Giant Panda in Wolong Natural Reserve","volume":"14","author":"Wei","year":"1994","journal-title":"Acta Theriol. Sin."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jpba.2010.07.021","article-title":"1H NMR Spectroscopic Studies on Human Seminal Plasma: A Probative Discriminant Function Analysis Classification Model","volume":"54","author":"Gupta","year":"2011","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2164\/jandrol.106.001545","article-title":"Relationships between Serum Hormone Levels and Semen Quality among Men from an Infertility Clinic","volume":"28","author":"Meeker","year":"2007","journal-title":"J. Androl."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"426","DOI":"10.4103\/1008-682X.175781","article-title":"Seminal Biomarkers for the Evaluation of Male Infertility","volume":"18","author":"Bieniek","year":"2016","journal-title":"Asian J. Androl."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.reprotox.2008.12.007","article-title":"Effects of Environmental Tobacco Smoke In Vivo on Rhesus Monkey Semen Quality, Sperm Function, and Sperm Metabolism","volume":"27","author":"Hung","year":"2009","journal-title":"Reprod. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.cca.2015.09.001","article-title":"Metabolic Characterization of Asthenozoospermia Using Nontargeted Seminal Plasma Metabolomics","volume":"450","author":"Zhang","year":"2015","journal-title":"Clin. Chim. Acta"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1002\/j.1939-4640.2003.tb02666.x","article-title":"Seasonal Variation and Age-Related Changes in Human Semen Parameters","volume":"24","author":"Chen","year":"2003","journal-title":"J. Androl."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/0011-2240(80)90055-3","article-title":"An Evaluation of Cryoprotective Compounds on Bovine Spermatozoa","volume":"17","author":"Jeyendran","year":"1980","journal-title":"Cryobiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103038","DOI":"10.1016\/j.jevs.2020.103038","article-title":"Metabolomic Analysis of Seminal Plasma in Stallions Belonging to Two Different Horse Breeds","volume":"89","author":"Bazzano","year":"2020","journal-title":"J. Equine Vet. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1095\/biolreprod.116.140707","article-title":"Lactate and Pyruvate Are Major Sources of Energy for Stallion Sperm with Dose Effects on Mitochondrial Function, Motility, and ROS Production","volume":"95","author":"Darr","year":"2016","journal-title":"Biol. Reprod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4236\/asm.2012.24008","article-title":"D-Aspartate, a Key Element for the Improvement of Sperm Quality","volume":"2","author":"Ronsini","year":"2012","journal-title":"Adv. Sex. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1016\/j.fertnstert.2005.05.019","article-title":"Occurrence of D-Aspartic Acid in Human Seminal Plasma and Spermatozoa: Possible Role in Reproduction","volume":"84","author":"Ronsini","year":"2005","journal-title":"Fertil. Steril."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0024-3205(96)00266-4","article-title":"Involvement of D-Aspartic Acid in the Synthesis of Testosterone in Rat Testes","volume":"59","author":"Annunziato","year":"1996","journal-title":"Life Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.anireprosci.2009.07.009","article-title":"Dl-Aspartic Acid Administration Improves Semen Quality in Rabbit Bucks","volume":"118","author":"Macchia","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3109\/01485018008987003","article-title":"Glucose Content as a Parameter of Semen Quality","volume":"5","author":"Martikainen","year":"1980","journal-title":"Arch. Androl."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1590\/S0102-09352005000100008","article-title":"Correlation between Fertility and Levels of Protein, Sugar and Free Amino Acids in Seminal Plasma of Nelore Bulls","volume":"57","author":"Torres","year":"2005","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref_48","first-page":"381","article-title":"Comparison of Spermatological Characteristics and Biochemical Seminal Plasma Parameters of Normozoospermic and Oligoasthenozoospermic Bulls of Two Breeds","volume":"31","author":"Tuncer","year":"2007","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Engel, K.M., Baumann, S., Rolle-Kampczyk, U., Schiller, J., von Bergen, M., and Grunewald, S. (2019). Metabolomic Profiling Reveals Correlations between Spermiogram Parameters and the Metabolites Present in Human Spermatozoa and Seminal Plasma. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0211679"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/S0026-0495(98)90028-2","article-title":"Relationships between Phenylalanine Hydroxylation and Plasma Aromatic Amino Acid Concentrations in Humans","volume":"47","author":"Barazzoni","year":"1998","journal-title":"Metab. Clin. Exp."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/imj.1959.8.3.218","article-title":"Plasma Creatinine Level and Creatinine Clearance as Tests of Renal Function","volume":"8","author":"Edwards","year":"1959","journal-title":"Australas. Ann. Med."},{"key":"ref_52","first-page":"192","article-title":"Relationship between Creatine Kinase Activity and Semen Characteristics in Subfertile Men","volume":"43","author":"Sidhu","year":"1998","journal-title":"Int. J. Fertil. Women\u2019s Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/S0090-4295(01)01224-9","article-title":"Creatine Kinase as an Indicator of Sperm Quality and Maturity in Men with Oligospermia","volume":"58","author":"Hallak","year":"2001","journal-title":"Urology"},{"key":"ref_54","first-page":"551","article-title":"Ferritin: Design and Formation of an Iron-Storage Molecule","volume":"304","author":"Ford","year":"1984","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1093\/humrep\/13.1.132","article-title":"Quantification by Magnetic Resonance Spectroscopy of Metabolites in Seminal Plasma Able to Differentiate Different Forms of Azoospermia","volume":"13","author":"Hamamah","year":"1998","journal-title":"Hum. Reprod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/mrd.22450","article-title":"Fertility-Associated Metabolites in Bull Seminal Plasma and Blood Serum: 1H Nuclear Magnetic Resonance Analysis","volume":"82","author":"Kumar","year":"2015","journal-title":"Mol. Reprod. Dev."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1093\/oxfordjournals.humrep.a137441","article-title":"Variations in Semen Parameters from Fathers","volume":"6","author":"Cooper","year":"1991","journal-title":"Hum. Reprod."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"878374","DOI":"10.1155\/2013\/878374","article-title":"Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status","volume":"2013","author":"Schreier","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"16585","DOI":"10.1021\/acsomega.8b01761","article-title":"Impact of Sample Storage on the NMR Fecal Water Metabolome","volume":"3","author":"Alegra","year":"2018","journal-title":"ACS Omega"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/12\/1536\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:30:47Z","timestamp":1760139047000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/12\/1536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,14]]},"references-count":59,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["ani12121536"],"URL":"https:\/\/doi.org\/10.3390\/ani12121536","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,14]]}}}