{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:50:56Z","timestamp":1780573856023,"version":"3.54.1"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32160823"],"award-info":[{"award-number":["32160823"]}]},{"name":"National Natural Science Foundation of China","award":["31460646"],"award-info":[{"award-number":["31460646"]}]},{"name":"National Natural Science Foundation of China","award":["XJ2021G179"],"award-info":[{"award-number":["XJ2021G179"]}]},{"name":"National Natural Science Foundation of China","award":["XJAUGRI2021006"],"award-info":[{"award-number":["XJAUGRI2021006"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang","award":["32160823"],"award-info":[{"award-number":["32160823"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang","award":["31460646"],"award-info":[{"award-number":["31460646"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang","award":["XJ2021G179"],"award-info":[{"award-number":["XJ2021G179"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang","award":["XJAUGRI2021006"],"award-info":[{"award-number":["XJAUGRI2021006"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang Agricultural University","award":["32160823"],"award-info":[{"award-number":["32160823"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang Agricultural University","award":["31460646"],"award-info":[{"award-number":["31460646"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang Agricultural University","award":["XJ2021G179"],"award-info":[{"award-number":["XJ2021G179"]}]},{"name":"Postgraduate research and innovation projects of Xinjiang Agricultural University","award":["XJAUGRI2021006"],"award-info":[{"award-number":["XJAUGRI2021006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Both the jejunum and colon release cytokines that interact with intestinal microbiota. However, it is largely unclear which cytokines and microbial populations are involved in the homeostasis of the intestinal ecosystem for sheep health. To address this, we collected contents for isolating microbiota and tissues for determining cytokines from the jejunum and colon of 7-month-old Altay sheep. We used the techniques of 16S rRNA sequencing and ELISA to detect microbial population and cytokine level, respectively. Correlations between microbial population and cytokines were analyzed by Spearman correlation coefficient. The correlation analysis revealed higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunum, and IL-6 and IL-12 levels were higher in the jejunum than in the colon. Association analysis further revealed a positive association between IL-10 level and both Ruminococcus_2 and norank_f_Bifidobacteriaceae population in the jejunum. The analysis also revealed positive associations between IL-6 level and Ruminococcaceae_UCG-014 and Ruminococcaceae_UCG-013 population, IL-10 and Prevotellaceae_UCG-004, as well as TNF-\u03b1 and Prevotellaceae_UCG-003 in the colon. These results indicate a potential interaction between the intestinal microbiota and the host immune system that needs to be further clarified for considering dietary formulations to maintain animal health and disease prevention.<\/jats:p>","DOI":"10.3390\/ani12121564","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T05:25:11Z","timestamp":1655443511000},"page":"1564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7329-399X","authenticated-orcid":false,"given":"Mengjun","family":"Ye","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qimin","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-4746","authenticated-orcid":false,"given":"Jinquan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","first-page":"26050","article-title":"The composition of the gut microbiota throughout life, with an emphasis on early life","volume":"26","author":"Murphy","year":"2015","journal-title":"Microb. Ecol. Health Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.chom.2019.08.011","article-title":"Regional Diversity of the Gastrointestinal Microbiome","volume":"26","author":"Martinez","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.immuni.2019.03.017","article-title":"Cytokine Networks in the Pathophysiology of Inflammatory Bowel Disease","volume":"50","author":"Friedrich","year":"2019","journal-title":"Immunity"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3748\/wjg.v24.i1.5","article-title":"Relationship between intestinal microbiota and ulcerative colitis: Mechanisms and clinical application of probiotics and fecal microbiota transplantation","volume":"24","author":"Shen","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/nrgastro.2016.165","article-title":"Diet, microorganisms and their metabolites, and colon cancer","volume":"13","year":"2016","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.3389\/fmicb.2017.01812","article-title":"Studying the Differences of Bacterial Metabolome and Microbiome in the Colon between Landrace and Meihua Piglets","volume":"8","author":"Yan","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"100","DOI":"10.5713\/ajas.16.0166","article-title":"Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis","volume":"30","author":"Wang","year":"2017","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3389\/fmicb.2018.00032","article-title":"The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"639030","DOI":"10.3389\/fgene.2021.639030","article-title":"Comparative Transcriptome Analysis of Key Genes and Pathways Activated in Response to Fat Deposition in Two Sheep Breeds with Distinct Tail Phenotype","volume":"12","author":"Zhang","year":"2021","journal-title":"Front. Genet."},{"key":"ref_10","first-page":"31","article-title":"Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits","volume":"11","author":"Elisa","year":"2021","journal-title":"Animals"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"455","DOI":"10.5009\/gnl16308","article-title":"Inflammatory Bowel Disease: Updates on Molecular Targets for Biologics","volume":"11","author":"Katsanos","year":"2017","journal-title":"Gut Liver"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1515\/jbcpp-2018-0036","article-title":"Immune response and inflammatory pathway of ulcerative colitis","volume":"30","author":"Chatuphonprasert","year":"2018","journal-title":"J. Basic Clin. Physiol. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1038\/s41467-018-03495-3","article-title":"Calcineurin-mediated IL-2 production by CD11c (high) MHCII (+) myeloid cells is crucial for intestinal immune homeostasis","volume":"9","author":"Mencarelli","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1146\/annurev-immunol-042617-053055","article-title":"Translating Immunology into Therapeutic Concepts for Inflammatory Bowel Disease","volume":"36","author":"Uhlig","year":"2018","journal-title":"Annu. Rev. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5632","DOI":"10.1111\/jcmm.14471","article-title":"Spontaneous colitis in IL-10-deficient mice was ameliorated via inhibiting glutaminase1","volume":"23","author":"Li","year":"2019","journal-title":"J. Cell. Mol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41385-019-0252-3","article-title":"The development of colitis in Il10 (-\/-) mice is dependent on IL-22","volume":"13","author":"Gunasekera","year":"2020","journal-title":"Mucosal Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13048-020-00670-3","article-title":"Role of gut microbiota in the development of insulin resistance and the mechanism underlying polycystic ovary syndrome: A review","volume":"13","author":"He","year":"2020","journal-title":"J. Ovarian Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chang, J., Yao, X., Zuo, C., Qi, Y., Chen, D., and Ma, W. (2020). The gut bacterial diversity of sheep associated with different breeds in Qinghai province. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02477-2"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/jam.14468","article-title":"The composition and stability of the faecal microbiota of Merino sheep","volume":"128","author":"Mamun","year":"2020","journal-title":"J. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1128\/AEM.00993-10","article-title":"Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes","volume":"77","author":"Evans","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1007\/s00284-013-0336-3","article-title":"Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing","volume":"67","author":"Thoetkiattikul","year":"2013","journal-title":"Curr. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/1751-7915.12462","article-title":"Diversity and functions of the sheep faecal microbiota: A multi-omic characterization","volume":"10","author":"Tanca","year":"2017","journal-title":"Microb. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1128\/JB.00125-06","article-title":"Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis: Regulation by carbon source and oxygen","volume":"188","author":"Spence","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Jami, E., and Mizrahi, I. (2012). Composition and similarity of bovine rumen microbiota across individual animals. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033306"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Han, X., Yang, Y., Yan, H., Wang, X., Qu, L., and Chen, Y. (2015). Rumen bacterial diversity of 80 to 110-day-old goats using 16S rRNA sequencing. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0117811"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13568-017-0378-1","article-title":"Microbial community compositions in the gastrointestinal tract of Chinese Mongolian sheep using Illumina MiSeq sequencing revealed high microbial diversity","volume":"7","author":"Zeng","year":"2017","journal-title":"AMB Express"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s13353-014-0258-1","article-title":"First insights into the microbial diversity in the omasum and reticulum of bovine using Illumina sequencing","volume":"56","author":"Peng","year":"2015","journal-title":"J. Appl. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s13063-020-4108-7","article-title":"A randomized controlled trial investigating the effect of a diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols on the intestinal microbiome and inflammation in patients with ulcerative colitis: Study protocol for a randomized controlled trial","volume":"21","author":"Milajerdi","year":"2020","journal-title":"Trials"},{"key":"ref_29","first-page":"723","article-title":"Relationship between intestinal flora and inflammatory factors in patients with nonalcoholic steatohepatitis","volume":"15","author":"Zhang","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.cell.2016.10.020","article-title":"Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity","volume":"167","author":"Schirmer","year":"2016","journal-title":"Cell"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/12\/1564\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:33:40Z","timestamp":1760139220000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/12\/1564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,17]]},"references-count":30,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["ani12121564"],"URL":"https:\/\/doi.org\/10.3390\/ani12121564","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,17]]}}}