{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:54:40Z","timestamp":1760237680270,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science, Innovation, and Universities","award":["RTI2018-098530-B-I00"],"award-info":[{"award-number":["RTI2018-098530-B-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although they did not match any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. The isolates shared 99.4 to 100% 16S rRNA gene sequence similarity among the strains and 96.5% similarity with Corynebacterium tapiri 2385\/12T, which was the closest phylogenetically related species. The DNA G+C content was 58.4 mol%. The major fatty acids were C15:0, C16:0, C17:1 \u03c98c and C18:1 \u03c99c, while the predominant mycolic acids consisted of C30:0, C32:0 and C34:0. The isolates were distinguished from related Corynebacterium species by a number of phenotypic properties. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from horses be classified in the genus Corynebacterium as Corynebacterium conjunctivae sp. nov. The type strain of C. conjunctivae is ICM19-01138T (DSM 109759T = CCUG 73728T).<\/jats:p>","DOI":"10.3390\/ani12141827","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T11:32:23Z","timestamp":1658143943000},"page":"1827","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Corynebacterium conjunctivae: A New Corynebacterium Species Isolated from the Ocular Surface of Healthy Horses"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1597-0643","authenticated-orcid":false,"given":"Jos\u00e9","family":"Fern\u00e1ndez-Garayz\u00e1bal","sequence":"first","affiliation":[{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Veterinary School, Complutense University, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacey","family":"LaFrentz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Auburn University, Auburn, AL 36894, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almudena","family":"Casamayor","sequence":"additional","affiliation":[{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Abarca","sequence":"additional","affiliation":[{"name":"Servei d\u2019Oftalmologia, ARS Veterinaria, 08034 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0717-6055","authenticated-orcid":false,"given":"Haitham","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary","family":"Cuming","sequence":"additional","affiliation":[{"name":"Scone Equine Hospital, Scone, NSW 2337, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cova","family":"Arias","sequence":"additional","affiliation":[{"name":"School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36894, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Veterinary School, Complutense University, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Vela","sequence":"additional","affiliation":[{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Veterinary School, Complutense University, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.4314\/ovj.v6i3.9","article-title":"Antimicrobial susceptibility and minimal inhibitory concentration of Pseudomonas aeruginosa isolated from septic ocular surface disease in different animal species","volume":"6","author":"Leigue","year":"2016","journal-title":"Open Vet. 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