{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:54:47Z","timestamp":1792788887231,"version":"4.5.0"},"reference-count":37,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T00:00:00Z","timestamp":1659139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Palital Feed Additives B.V."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Short chain fatty acid (SCFA) butyrate has various beneficial effects on the gut microbiota as well as on the overall health status and metabolism of the host organism. The modulatory role of butyrate on gut barrier integrity reflected by tight junction protein expression has been already described in mammalian species. However, there is limited information available regarding chickens. Therefore, the main aim of this study was to monitor the effects of protected butyrate on claudin barrier protein and monocarboxylate transporter 1 abundance in different gastrointestinal segments of chickens as well as the growth performance of broiler chickens. The effect of protected butyrate on the caecal microbiota was monitored by quantifying the concentrations of total eubacteria and key enzymes of butyrate production. Furthermore, intestinal SCFA concentrations were also measured. Based on the data obtained, protected butyrate increased the overall performance as well as the barrier integrity of various gut segments. Protected butyrate also positively affected the SCFA concentration and composition. These findings provide valuable insight into the complex effects of protected butyrate on broiler gut health, highlighting the beneficial effects in improving intestinal barrier integrity and performance parameters.<\/jats:p>","DOI":"10.3390\/ani12151940","type":"journal-article","created":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T21:49:02Z","timestamp":1659304142000},"page":"1940","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-9444","authenticated-orcid":false,"given":"G\u00e1bor","family":"M\u00e1tis","sequence":"first","affiliation":[{"name":"Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, Istv\u00e1n utca 2, H-1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5992-6797","authenticated-orcid":false,"given":"M\u00e1t\u00e9","family":"Mackei","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, Istv\u00e1n utca 2, H-1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Boomsma","sequence":"additional","affiliation":[{"name":"Palital Feed Additives B.V., De Tweede Geerden, 5334 LH Velddriel, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9867-0664","authenticated-orcid":false,"given":"Hedvig","family":"F\u00e9bel","sequence":"additional","affiliation":[{"name":"Nutrition Physiology Research Group, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Geszteny\u00e9s Str. 1, H-2053 Herceghalom, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Nadolna","sequence":"additional","affiliation":[{"name":"Department of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Szyma\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0759-633X","authenticated-orcid":false,"given":"Joan E.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Palital Feed Additives B.V., De Tweede Geerden, 5334 LH Velddriel, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0139-1942","authenticated-orcid":false,"given":"Zsuzsanna","family":"Neogr\u00e1dy","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, Istv\u00e1n utca 2, H-1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Koz\u0142owski","sequence":"additional","affiliation":[{"name":"Department of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Melaku, M., Zhong, R., Han, H., Wan, F., Yi, B., and Zhang, H. 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