{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T23:10:40Z","timestamp":1774480240588,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gansu Provincial Finance Department Project","award":["033-041041"],"award-info":[{"award-number":["033-041041"]}]},{"name":"Gansu Provincial Finance Department Project","award":["32060140"],"award-info":[{"award-number":["32060140"]}]},{"name":"National Natural Science Foundation Project of China","award":["033-041041"],"award-info":[{"award-number":["033-041041"]}]},{"name":"National Natural Science Foundation Project of China","award":["32060140"],"award-info":[{"award-number":["32060140"]}]},{"name":"Major Scientific and Technology Project of Qingyang City","award":["033-041041"],"award-info":[{"award-number":["033-041041"]}]},{"name":"Major Scientific and Technology Project of Qingyang City","award":["32060140"],"award-info":[{"award-number":["32060140"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Keratin-associated proteins (KAPs) are a structural component of cashmere fibers and in part determine fiber attributes. The gene encoding the high-glycine\/tyrosine KAP6-2 (called KRTAP6-2) has been described in sheep, but it has not been identified goats. In this study, a 252-bp open reading frame with similarity to ovine KRTAP6-2 was found on goat chromosome 1, with its upstream and downstream flanking sequences are closely related with ovine KRTAP6-2 but are clearly distinct from other ovine KRTAP6-n sequences. Polymerase chain reaction amplification followed by single strand conformation polymorphism analysis of this region revealed five distinct banding patterns representing five different sequences (A to E) in 230 Longdong cashmere goats. Eleven diallelic single nucleotide polymorphisms (SNPs), a three-nucleotide sequence variation, and a 12-bp insertion\/deletion were found among these five sequences, with most SNPs being either outside the coding region or synonymous. The presence of variant D was found to be associated with decreased mean fiber diameter (MFD; present: 13.26 \u00b1 0.07 \u00b5m; absent: 13.55 \u00b1 0.04 \u00b5m; p &lt; 0.001), suggesting that variation in KRTAP6-2 may affect fiber diameter and have value as a molecular marker for improving the cashmere fiber diameter trait.<\/jats:p>","DOI":"10.3390\/ani12162040","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T01:58:30Z","timestamp":1660183110000},"page":"2040","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sequence Variation in Caprine KRTAP6-2 Affects Cashmere Fiber Diameter"],"prefix":"10.3390","volume":"12","author":[{"given":"Jian","family":"Cao","sequence":"first","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9811-0085","authenticated-orcid":false,"given":"Jiqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7620-3160","authenticated-orcid":false,"given":"Huitong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Gene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Hu","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiu","family":"Liu","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6688-1117","authenticated-orcid":false,"given":"Shaobin","family":"Li","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2587-5826","authenticated-orcid":false,"given":"Yuzhu","family":"Luo","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon G. H.","family":"Hickford","sequence":"additional","affiliation":[{"name":"Gene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, J., Che, L., Hickford, J.G.H., Zhou, H., Hao, Z., Luo, Y., Hu, J., Liu, X., and Li, S. (2017). Identification of the caprine keratin-associated protein 20-2 (KAP20-2) gene and its effect on cashmere traits. Genes, 8.","DOI":"10.3390\/genes8110328"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0301-6226(93)90241-9","article-title":"Heritabilities and genetic correlations for live weight and fibre traits in New Zealand Cashmere goats","volume":"33","author":"Bigham","year":"1993","journal-title":"Livest. Prod. 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