{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:27:06Z","timestamp":1785864426860,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:00:00Z","timestamp":1660435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Austrian ministries BMVIT and BMDW"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In a low-fibre diet destined for broilers, the effects of two lignocellulose products and soybean hulls were evaluated regarding their effect on ileal morphometric parameters, caecal gene expression, foot pad dermatitis, and performance. A total of 5040-day-old broilers (Ross 308) were allotted to four treatments and fattened for 36 days applying a three-phase feeding program. The control diet consisted of corn, wheat, and soybean meal. Experimental diets were supplemented with 0.8% lignocellulose product 1, 0.8% lignocellulose product 2, or 1.6% soybean hulls. Tissue samples for caecal expression of inflammation-related genes and ileal morphometries were collected on day 21. Gizzard pH and weights were recorded, and foot pad scores were evaluated at day of slaughter (day 36). In starter (day 1\u201310) and finisher phase (day 28\u201336), no effect on the performance was observed. In grower phase (day 11\u201327), fibre-supplemented diets showed significantly heavier body weights and daily weight gains (p &lt; 0.05). Daily feed intake, feed conversion ratio, and gene expression analysis were unaffected by dietary fibre supplementation. Positive effects regarding ileal morphometrics (higher villi) and foot pad health occurred in fibre-supplemented diets. In conclusion, fibre supplementation improved performance in grower phase and showed beneficial effects regarding ileal morphology and foot pad dermatitis.<\/jats:p>","DOI":"10.3390\/ani12162069","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T21:09:06Z","timestamp":1660511346000},"page":"2069","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Influence of Insoluble Dietary Fibre on Expression of Pro-Inflammatory Marker Genes in Caecum, Ileal Morphology, Performance, and Foot Pad Dermatitis in Broiler"],"prefix":"10.3390","volume":"12","author":[{"given":"Mariella","family":"Liebl","sequence":"first","affiliation":[{"name":"FFoQSI GmbH, Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, 3430 Tulln, Austria"},{"name":"Department of Agrobiotechnology, Institute of Animal Nutrition, Livestock Products and Nutrition Physiology, University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6909-0356","authenticated-orcid":false,"given":"Martin","family":"Gierus","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology, Institute of Animal Nutrition, Livestock Products and Nutrition Physiology, University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Potthast","sequence":"additional","affiliation":[{"name":"Agromed Austria GmbH, 4550 Kremsm\u00fcnster, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-4015","authenticated-orcid":false,"given":"Karl","family":"Schedle","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology, Institute of Animal Nutrition, Livestock Products and Nutrition Physiology, University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3382\/japr.2011-00477","article-title":"Poultry response to high levels of dietary fibre sources varying in physical and chemical characteristics","volume":"21","author":"Mateos","year":"2012","journal-title":"J. Appl. Poult. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0377-8401(03)00163-9","article-title":"A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals","volume":"108","author":"Montagne","year":"2003","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3382\/ps\/pey345","article-title":"Effects of dietary supplementation of the lignocelluloses FibreCell and OptiCell on performance, expression of inflammation-related genes and the gut microbiome of broilers","volume":"98","author":"Zeitz","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.anifeedsci.2018.10.008","article-title":"The effect of microbiome modulation on the intestinal health of poultry","volume":"250","author":"Kogut","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Tejeda, O.J., and Kim, W.K. (2021). Role of Dietary Fiber in Poultry Nutrition. Animals, 11.","DOI":"10.3390\/ani11020461"},{"key":"ref_6","first-page":"531","article-title":"Effects of insoluble fibre on growth performance, carcass traits and ileum morphological parameters on broiler chick males","volume":"12","author":"Sarikhan","year":"2010","journal-title":"Int. J. Agric. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1002\/jsfa.9612","article-title":"Physicochemical properties to support fibre characterization in monogastric animal nutrition","volume":"99","author":"Slama","year":"2019","journal-title":"J. Sci. Food Agri."},{"key":"ref_8","unstructured":"(1999). Society of Nutrition Physiology. Empfehlungen zur Energie- und N\u00e4hrstoffversorgung der Legehennen und Masth\u00fchner (Broiler). Ausschuss f\u00fcr Bedarfsnormen der Gesellschaft f\u00fcr Ern\u00e4hrungsphysiologie, DLG-Verlag."},{"key":"ref_9","unstructured":"Butterworth, A., Arnould, C., van Niekerk, T., Veissier, I., and Keeling, L. (2009). Welfare Quality\u00ae Assessment Protocol for Poultry (Broilers, Laying Hens), ASG Wageningen University and Research Centre."},{"key":"ref_10","unstructured":"Naumann, C., and Bassler, R. (2012). Die Chemische Untersuchung von Futtermitteln, VDLUFA-Verlag."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/00015128909438504","article-title":"Determination of nitrogen as elementary N, an alternative to Kjeldahl","volume":"39","author":"Hansen","year":"1989","journal-title":"Acta Agric. Scand."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.3382\/ps.2013-03783","article-title":"Effects of grinding method, particle size, and physical form of the diet on gastrointestinal morphology and jejunal glucose transport in laying hens","volume":"93","author":"Ruhnke","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.vetimm.2007.12.002","article-title":"Identification and validation of housekeeping genes as internal control for gene expression in an intravenous LPS inflammation model in chickens","volume":"122","author":"Vangestel","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Liu, J., Liu, L., Li, L., Tian, D., Li, W., Xu, L., Yan, R., Li, X., and Song, X. (2018). Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1665-z"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.aninu.2020.04.003","article-title":"Priming of intestinal cytoprotective genes and antioxidant capacity by dietary phytogenic inclusion in broilers","volume":"6","author":"Mountzouris","year":"2020","journal-title":"Anim. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_18","first-page":"45","article-title":"Sustainable Pig and Poultry Nutrition by Improvement of Nutrient Utilisation\u2014A Review","volume":"67","author":"Schedle","year":"2016","journal-title":"Bodenkultur"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1017\/S1751731119003343","article-title":"A diet containing native or fermented wheat bran does not interfere with natural microbiota of laying hens","volume":"14","author":"Zitz","year":"2020","journal-title":"Animal"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s40104-021-00576-0","article-title":"Dietary fibre in poultry nutrition and their effects on nutrient utilization, performance, gut health, and on the environment: A review","volume":"12","author":"Jha","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_21","first-page":"83","article-title":"Effects of reducing growth rate via diet dilution on bone mineralization, performance and carcass yield of coccidia-infected broilers","volume":"25","author":"Oikeh","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s40104-021-00594-y","article-title":"Lignocellulose as an insoluble fibre source in poultry nutrition: A review","volume":"12","author":"Zentek","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1080\/00071668.2017.1380295","article-title":"Effect of particle size distribution of maize and soybean meal on the precaecal amino acid digestibility in broiler chickens","volume":"59","author":"Siegert","year":"2018","journal-title":"Br. Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1093\/ps\/86.8.1705","article-title":"Effect of type of cereal, heat processing of the cereal, and inclusion of fibre in the diet on productive performance and digestive traits of broilers","volume":"86","author":"Mateos","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_25","first-page":"83","article-title":"Performances, ileal and cecal microbial populations and histological characteristics in broilers fed diets supplemented with lignocellulose","volume":"25","author":"Makivic","year":"2019","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1111\/jpn.12238","article-title":"Gender-specific effects of a phytogenic feed additive on performance, intestinal physiology and morphology in broiler chickens","volume":"99","author":"Humer","year":"2014","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.3382\/ps\/pex123","article-title":"Coarse particle inclusion and lignocellulose-rich fibre addition in feed benefit performance and health of broiler chickens","volume":"96","author":"Kheravii","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_28","unstructured":"Mateos, G., Guzman, P., Saldana, B., Bonilla, A., Lazar, R., and Jimenez-Moreno, E. (2013, January 26\u201329). Relevance of dietary fibre in poultry feeding. Proceedings of the 19th European Symposium on Poultry Nutrition, Potsdam, Germany."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2141\/jpsa.44.175","article-title":"Influence of feed form on gizzard morphology, particle size spectra of duodenal digesta in broiler chickens","volume":"44","author":"Amerah","year":"2007","journal-title":"J. Poult Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1080\/00071668.2021.1919995","article-title":"Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers","volume":"62","author":"Morgan","year":"2021","journal-title":"Br. Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s13567-018-0538-6","article-title":"Biomarkers for monitoring intestinal health in poultry: Present status and future perspectives","volume":"49","author":"Ducatelle","year":"2018","journal-title":"Vet. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3382\/ps.2011-01352","article-title":"The influence of different levels of micronized insoluble fibre on broiler performance and litter moisture","volume":"90","author":"Rezaei","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.3382\/ps.2010-00770","article-title":"Footpad dermatitis in poultry","volume":"89","author":"Shepherd","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6828","DOI":"10.1016\/j.psj.2020.08.081","article-title":"The effects of cellulose and soybean hulls as sources of dietary fibre on the growth performance, organ growth, gut histomorphology, and nutrient digestibility of broiler chickens","volume":"99","author":"Tejeda","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"104189","DOI":"10.1016\/j.livsci.2020.104189","article-title":"Small intestine morphology and ileal biogenic amines content in broiler chickens fed diets supplemented with lignocellulose","volume":"241","author":"Bogucka","year":"2020","journal-title":"Livest. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.3382\/ps.2010-813","article-title":"Digestive tract measurements and histological adaptation in broiler lines divergently selected for digestive efficiency","volume":"89","author":"Verdal","year":"2010","journal-title":"Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/16\/2069\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:08:35Z","timestamp":1760141315000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/16\/2069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,14]]},"references-count":36,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ani12162069"],"URL":"https:\/\/doi.org\/10.3390\/ani12162069","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,14]]}}}