{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:43:00Z","timestamp":1789062180402,"version":"build-2803163510"},"reference-count":77,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:00:00Z","timestamp":1660435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Office of the Ministry of Higher Education, Science, Research and Innovation","award":["RUP2.2\/Con-CASAF PD 03"],"award-info":[{"award-number":["RUP2.2\/Con-CASAF PD 03"]}]},{"name":"the Office of the Ministry of Higher Education, Science, Research and Innovation","award":["#N42A650553"],"award-info":[{"award-number":["#N42A650553"]}]},{"name":"National Research Council of Thailand (NRCT)","award":["RUP2.2\/Con-CASAF PD 03"],"award-info":[{"award-number":["RUP2.2\/Con-CASAF PD 03"]}]},{"name":"National Research Council of Thailand (NRCT)","award":["#N42A650553"],"award-info":[{"award-number":["#N42A650553"]}]},{"name":"Kasetsart University Research and Development Institute (KURDI) Bangkok","award":["RUP2.2\/Con-CASAF PD 03"],"award-info":[{"award-number":["RUP2.2\/Con-CASAF PD 03"]}]},{"name":"Kasetsart University Research and Development Institute (KURDI) Bangkok","award":["#N42A650553"],"award-info":[{"award-number":["#N42A650553"]}]},{"name":"Faculty of Veterinary Medicine, Kasetsart University","award":["RUP2.2\/Con-CASAF PD 03"],"award-info":[{"award-number":["RUP2.2\/Con-CASAF PD 03"]}]},{"name":"Faculty of Veterinary Medicine, Kasetsart University","award":["#N42A650553"],"award-info":[{"award-number":["#N42A650553"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Feline infectious peritonitis (FIP) is a worldwide fatal disease caused by a mutant feline coronavirus (FCoV). Simple and efficient molecular detection methods are needed. Here, sensitive, specific, rapid, and reliable colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect the ORF1a\/1b gene of FCoV from cats with suspected FIP using neutral red as an indicator. Novel LAMP primers were specifically designed based on the gene of interest. The isothermal assay could visually detect FCoV at 58 \u00b0C for 50 min. The RT-LAMP assay was highly specific and had no cross-reactivity with other related feline viruses. The detection limit of FCoV detection by RT-LAMP was 20 fg\/\u00b5L. A blind clinical test (n = 81) of the developed RT-LAMP procedure was in good agreement with the conventional PCR method. In the light of its performance specificity, sensitivity, and easy visualization, this neutral-red-based RT-LAMP approach would be a fruitful alternative molecular diagnostic tool for veterinary inspection of FCoV when combined with nucleotide sequencing or specific PCR to affirm the highly virulent FIP-associated FCoV.<\/jats:p>","DOI":"10.3390\/ani12162075","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T21:09:06Z","timestamp":1660511346000},"page":"2075","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Feline Coronavirus"],"prefix":"10.3390","volume":"12","author":[{"given":"Witsanu","family":"Rapichai","sequence":"first","affiliation":[{"name":"Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wichayet","family":"Saejung","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Graduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotchaporn","family":"Khumtong","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Graduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaiwat","family":"Boonkaewwan","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80161, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supansa","family":"Tuanthap","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Bangpra, Chonburi 20110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-0584","authenticated-orcid":false,"given":"Peter A.","family":"Lieberzeit","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Strasse 42, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2421-7859","authenticated-orcid":false,"given":"Kiattawee","family":"Choowongkomon","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2426-0961","authenticated-orcid":false,"given":"Jatuporn","family":"Rattanasrisomporn","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Graduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s44149-021-00005-9","article-title":"The Taxonomy, Host Range and Pathogenicity of Coronaviruses and Other Viruses in the Nidovirales Order","volume":"1","author":"Zhou","year":"2021","journal-title":"Anim. 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