{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T15:18:44Z","timestamp":1783091924065,"version":"3.54.6"},"reference-count":72,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P4-0053"],"award-info":[{"award-number":["P4-0053"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P4-0092"],"award-info":[{"award-number":["P4-0092"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P2-0185"],"award-info":[{"award-number":["P2-0185"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["Postgraduate Research Funding"],"award-info":[{"award-number":["Postgraduate Research Funding"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Bisphenol A (BPA) is a well-known synthetic compound that belongs to the group of endocrine-disrupting chemicals. Although bone tissue is a target for these compounds, studies on BPA-related effects on bone morphology in farm animals are limited. In this preliminary study, we investigated the effects of short-term dietary BPA exposure on femoral morphology, metabolism, mineral content, and biomechanical behavior in rams aged 9\u201312 months. Fourteen rams of the Istrian Pramenka breed were randomly divided into a BPA group and a control group (seven rams\/group) and exposed to 25 \u00b5g BPA\/kg bw for 64 days in feed. Blood was collected for determination of bone turnover markers (procollagen N-terminal propeptide, C-terminal telopeptide), and femurs were assessed via computed tomography, histomorphometry, three-point bending test, and mineral analysis. BPA had no significant effects on most of the parameters studied. Only mineral analysis showed decreased manganese (50%; p \u2264 0.05) and increased copper content (25%; p \u2264 0.05) in the femurs of BPA-exposed rams. These results suggest that a 2-month, low-dose exposure to BPA in growing rams did not affect the histomorphology, metabolism, and biomechanical behavior of femurs; however, it affected the composition of microelements, which could affect the histometric and biophysical properties of bone in the long term.<\/jats:p>","DOI":"10.3390\/ani12172179","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T21:28:12Z","timestamp":1661462892000},"page":"2179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Experimental Exposure to Bisphenol A Has Minimal Effects on Bone Tissue in Growing Rams\u2014A Preliminary Study"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7934-3302","authenticated-orcid":false,"given":"Jana","family":"Brankovi\u010d","sequence":"first","affiliation":[{"name":"Institute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Leskovec","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Dom\u017eale, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"\u0160turm","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesna","family":"Cerkvenik-Flajs","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u0161a","family":"\u0160terpin","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry and Biochemistry, University Medical Centre Ljubljana, Zalo\u0161ka Cesta 2, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1839-2415","authenticated-orcid":false,"given":"Jo\u0161ko","family":"Osredkar","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry and Biochemistry, University Medical Centre Ljubljana, Zalo\u0161ka Cesta 2, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Pharmacy, University of Ljubljana, A\u0161ker\u010deva 7, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estera","family":"Pogorevc","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Cesta v Mestni log 47, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1142-076X","authenticated-orcid":false,"given":"David","family":"Antolinc","sequence":"additional","affiliation":[{"name":"Chair for Testing in Materials and Structures, Faculty of Civil and Geodetic Engineering, University of Ljubljana, Jamova 2, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9968-9228","authenticated-orcid":false,"given":"Milka","family":"Vrecl","sequence":"additional","affiliation":[{"name":"Institute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s11154-019-09521-z","article-title":"Endocrine disrupting chemicals: Exposure, effects on human health, mechanism of action, models for testing and strategies for prevention","volume":"21","author":"Yilmaz","year":"2020","journal-title":"Rev. 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