{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T10:24:12Z","timestamp":1782987852243,"version":"3.54.5"},"reference-count":48,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Project of Science and Technology Program of Guizhou Province","award":["5411 2017"],"award-info":[{"award-number":["5411 2017"]}]},{"name":"Key Project of Science and Technology Program of Guizhou Province","award":["125 2019"],"award-info":[{"award-number":["125 2019"]}]},{"name":"Key Project of Science and Technology Program of Guizhou Province","award":["(2021) 294"],"award-info":[{"award-number":["(2021) 294"]}]},{"name":"Key Project of Science and Technology Program of Guizhou Province","award":["(2021) B16"],"award-info":[{"award-number":["(2021) B16"]}]},{"name":"World Top Discipline Program of Guizhou Province","award":["5411 2017"],"award-info":[{"award-number":["5411 2017"]}]},{"name":"World Top Discipline Program of Guizhou Province","award":["125 2019"],"award-info":[{"award-number":["125 2019"]}]},{"name":"World Top Discipline Program of Guizhou Province","award":["(2021) 294"],"award-info":[{"award-number":["(2021) 294"]}]},{"name":"World Top Discipline Program of Guizhou Province","award":["(2021) B16"],"award-info":[{"award-number":["(2021) B16"]}]},{"name":"Natural Science Research Project of Education Department of Guizhou Province","award":["5411 2017"],"award-info":[{"award-number":["5411 2017"]}]},{"name":"Natural Science Research Project of Education Department of Guizhou Province","award":["125 2019"],"award-info":[{"award-number":["125 2019"]}]},{"name":"Natural Science Research Project of Education Department of Guizhou Province","award":["(2021) 294"],"award-info":[{"award-number":["(2021) 294"]}]},{"name":"Natural Science Research Project of Education Department of Guizhou Province","award":["(2021) B16"],"award-info":[{"award-number":["(2021) B16"]}]},{"name":"Guizhou Normal University Academic New Seedling Fund project","award":["5411 2017"],"award-info":[{"award-number":["5411 2017"]}]},{"name":"Guizhou Normal University Academic New Seedling Fund project","award":["125 2019"],"award-info":[{"award-number":["125 2019"]}]},{"name":"Guizhou Normal University Academic New Seedling Fund project","award":["(2021) 294"],"award-info":[{"award-number":["(2021) 294"]}]},{"name":"Guizhou Normal University Academic New Seedling Fund project","award":["(2021) B16"],"award-info":[{"award-number":["(2021) B16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>EGF plays an important role in the intestinal repair and nutrients transport of animals. However, the effect of EGF on the intestinal health of piglets with IUGR has not been reported. Thus, the present study was performed to investigate the effects of EGF on the intestinal morphology, glucose absorption, antioxidant capacity, and barrier function of piglets with IUGR. A total of 6 NBW piglets and 12 IUGR piglets were randomly divided into three treatments: NC group (NBW piglets fed with basal diet, n = 6), IC group (IUGR piglets fed with basal diet, n = 6), and IE group (IUGR piglets fed with basal diet supplemented with 2 mg\/kg EGF, n = 6). Growth performance, serum biochemical profile, jejunum histomorphology, jejunum glucose absorption and antioxidant capacity, and jejunal barrier function were measured. The results showed that EGF supplementation significantly increased the final body weight (FBW), average daily gain (ADG), and average daily feed intake (ADFI) of piglets with IUGR; EGF supplementation significantly increased the total protein (TP), glucose (GLU), and immunoglobulin G (IgG) levels compared with the IUGR piglets in the IC group; EGF administration effectively exhibited an increased jejunum villus height (VH) and the villus-height-to-crypt-depth ratio (V\/C) of IUGR piglets compared with the IC group; EGF supplementation significantly increased sodium\/potassium-transporting adenosine triphosphatase (Na+\/K+-ATPase) activity, intestinal alkaline phosphatase (AKP) activity, glucose transporter sodium\/glucose cotransporter 1 (SGLT1), glucose transporter 2 (GLUT2), and AMP-activated protein kinase \u03b11 (AMPK-\u03b11) mRNA expressions in the jejunum of IUGR piglets compared with the IC group; EGF supplementation exhibited increased superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) levels, tended to increase glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and tended to decrease the malondialdehyde (MDA) level in the jejunum of IUGR piglets compared with the IC group; EGF supplementation significantly increased ZO-1, Claudin-1, Occludin, and MUC2 mRNA expressions and improved secreted immunoglobulin A (sIgA) secretion in the jejunum of IUGR piglets compared with the IC group and tended to decrease the interleukin 1\u03b2 (IL-1\u03b2), IL-6, and tumor necrosis factor \u03b1 (TNF-\u03b1) levels in the jejunum of IUGR piglets compared with the IC group. Pearson\u2019s correlation analysis further showed that EGF can promote intestinal development and nutrient absorption by promoting intestinal barrier function, thus improving the growth performance of IUGR piglets.<\/jats:p>","DOI":"10.3390\/ani12172245","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T21:25:18Z","timestamp":1661894718000},"page":"2245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dietary Epidermal Growth Factor Supplementation Alleviates Intestinal Injury in Piglets with Intrauterine Growth Retardation via Reducing Oxidative Stress and Enhancing Intestinal Glucose Transport and Barrier Function"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4789-5687","authenticated-orcid":false,"given":"Xiaopeng","family":"Tang","sequence":"first","affiliation":[{"name":"State Engineering Technology Institute for Karst Desertfication Control, School of Karst Science, Guizhou Normal University, No. 116 North Baoshan Road, Yunyan District, Guiyang 550001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangning","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Engineering Technology Institute for Karst Desertfication Control, School of Karst Science, Guizhou Normal University, No. 116 North Baoshan Road, Yunyan District, Guiyang 550001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2316","DOI":"10.2527\/jas.2006-156","article-title":"Board-invited review: Intrauterine growth retardation: Implications for the animal sciences","volume":"84","author":"Wu","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/jn\/138.1.60","article-title":"Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs","volume":"138","author":"Wang","year":"2008","journal-title":"J. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s40104-020-00490-x","article-title":"Intrauterine growth restriction alters growth performance, plasma hormones, and small intestinal microbial communities in growing-finishing pigs","volume":"11","author":"Xiong","year":"2020","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2644205","DOI":"10.1155\/2022\/2644205","article-title":"Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses","volume":"2022","author":"Tang","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Fung, C.M., White, J.R., Brown, A.S., Gong, H., Weitkamp, J.H., Frey, M.R., and McElroy, S.J. (2016). Intrauterine growth restriction alters mouse intestinal architecture during development. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146542"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s12711-018-0417-7","article-title":"Genetic selection against intrauterine growth retardation in piglets: A problem at the piglet level with a solution at the sow level","volume":"50","author":"Matheson","year":"2018","journal-title":"Genet. Sel. Evol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1007\/s00726-018-2608-5","article-title":"Maternal L-glutamine supplementation during late gestation alleviates intrauterine growth restriction-induced intestinal dysfunction in piglets","volume":"50","author":"Zhu","year":"2018","journal-title":"Amino Acids"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7178123","DOI":"10.1155\/2020\/7178123","article-title":"Protective Effect of Polydatin on Jejunal Mucosal Integrity, Redox Status, Inflammatory Response, and Mitochondrial Function in Intrauterine Growth-Retarded Weanling Piglets","volume":"2020","author":"Zhang","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8149","DOI":"10.1039\/C9FO01877H","article-title":"Flaxseed oil supplementation improves intestinal function and immunity, associated with altered intestinal microbiome and fatty acid profile in pigs with intrauterine growth retardation","volume":"10","author":"Che","year":"2019","journal-title":"Food Funct."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1927348","DOI":"10.1155\/2016\/1927348","article-title":"Epidermal Growth Factor and Intestinal Barrier Function","volume":"2016","author":"Tang","year":"2016","journal-title":"Mediators Inflamm."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"719877","DOI":"10.3389\/fmicb.2021.719877","article-title":"Potential Application of Lonicera japonica Extracts in Animal Production: From the Perspective of Intestinal Health","volume":"12","author":"Tang","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Olszewski, J., Zabielski, R., Skrzypek, T., Matyba, P., Wierzbicka, M., Adamski, A., Grzesiuk, E., Sady, M., Gajewski, Z., and Ferenc, K. (2021). Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets. Animals, 11.","DOI":"10.3390\/ani11040990"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s40104-021-00589-9","article-title":"Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota","volume":"12","author":"Chen","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"847673","DOI":"10.3389\/fnut.2022.847673","article-title":"Curcumin and Intestinal Oxidative Stress of Pigs with Intrauterine Growth Retardation: A Review","volume":"9","author":"Tang","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s11427-018-9519-6","article-title":"Epidermal growth factor improves intestinal morphology by stimulating proliferation and differentiation of enterocytes and mTOR signaling pathway in weaning piglets","volume":"63","author":"Wang","year":"2020","journal-title":"Sci. China Life Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tang, X., Liu, B., Wang, X., Yu, Q., and Fang, R. (2018). Epidermal growth factor, through alleviating oxidative stress, protect IPEC-J2 cells from lipopolysaccharides-induced apoptosis. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030848"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xue, J., Xie, L., Liu, B., Zhou, L., Hu, Y., Ajuwon, K.M., and Fang, R. (2021). Dietary Supplementation of EGF Ameliorates the Negatively Effects of LPS on Early-Weaning Piglets: From Views of Growth Performance, Nutrient Digestibility, Microelement Absorption and Possible Mechanisms. Animals, 11.","DOI":"10.3390\/ani11061598"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"417","DOI":"10.17582\/journal.pjz\/20200117080156","article-title":"Effects of epidermal growth factor on glutamine and glucose absorption by IPEC-J2 cells challenged by lipopolysaccharide using the Ussing chamber system","volume":"53","author":"Tang","year":"2021","journal-title":"Pak. J. Zool."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1038\/labinvest.2011.73","article-title":"Epidermal growth factor protects the apical junctional complexes from hydrogen peroxide in bile duct epithelium","volume":"91","author":"Guntaka","year":"2011","journal-title":"Lab. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.alcohol.2014.07.008","article-title":"Epidermal growth factor improved alcohol-induced inflammation in rats","volume":"48","author":"Chen","year":"2014","journal-title":"Alcohol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3574","DOI":"10.1074\/jbc.M709141200","article-title":"Role of phospholipase C\u03b3-induced activation of protein kinase C\u03f5 (PKC\u03f5) and PKC\u03b21 in epidermal growth factor-mediated protection of tight junctions from acetaldehyde in Caco-2 cell monolayers","volume":"283","author":"Suzuki","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.acthis.2013.07.005","article-title":"The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia\/reperfusion in rats","volume":"116","author":"Bolkent","year":"2014","journal-title":"Acta Histoch."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1080\/1828051X.2022.2073832","article-title":"Epidermal growth factor activates EGFR\/AMPK signalling to up-regulate the expression of SGLT1 and GLUT2 to promote intestinal glucose absorption in lipopolysaccharide challenged IPEC-J2 cells and piglets","volume":"21","author":"Tang","year":"2022","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_24","unstructured":"NRC (2012). Nutrient Requirements of Swine, National Academy Press. [11th ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"744630","DOI":"10.3389\/fvets.2021.744630","article-title":"Effects of Microbial Fermented Feed on Serum Biochemical Profile, Carcass Traits, Meat Amino Acid and Fatty Acid Profile, and Gut Microbiome Composition of Finishing Pigs","volume":"8","author":"Tang","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3177\/jnsv.66.311","article-title":"The effects of dietary porous zinc oxide supplementation on growth performance, inflammatory cytokines and tight junction\u2019s gene expression in early-weaned piglets","volume":"66","author":"Peng","year":"2020","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.17582\/journal.pjz\/2019.51.6.2167.2174","article-title":"Effects of calcitonin on porcine intestinal epithelial cells proliferation, phosphorus absorption, and NaPi-IIb expression","volume":"51","author":"Tang","year":"2019","journal-title":"Pak. J. Zool."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1017\/S1751731119002581","article-title":"Epidermal growth factor promotes intestinal secretory cell differentiation in weaning piglets via Wnt\/\u03b2-catenin signalling","volume":"14","author":"Wang","year":"2020","journal-title":"Animal"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1080\/09540105.2020.1728235","article-title":"Functional bioactive substance improves the growth performance, antioxidant capacity and immune function of growth retardation pigs","volume":"31","author":"Wang","year":"2020","journal-title":"Food Agric. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"skab186","DOI":"10.1093\/jas\/skab186","article-title":"Dietary dimethylglycine sodium salt supplementation improves growth performance, redox status, and skeletal muscle function of intrauterine growth-restricted weaned piglets","volume":"99","author":"Bai","year":"2021","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jbiotec.2014.01.012","article-title":"Growth performance of early-weaned pigs is enhanced by feeding epidermal growth factor-expressing Lactococcus lactis fermentation product","volume":"173","author":"Bedford","year":"2014","journal-title":"J. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1515\/cclm-2013-0096","article-title":"A systematic review of data on biological variation for alanine aminotransferase, aspartate aminotransferase and \u03b3-glutamyl transferase","volume":"51","author":"Carobene","year":"2013","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"902","DOI":"10.3168\/jds.S0022-0302(95)76704-2","article-title":"Plasma proteins and a probiotic as ingredients in milk replacer","volume":"78","author":"Morrill","year":"1995","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1017\/S0007114516000738","article-title":"Effects of dietary supplementation with epidermal growth factor-expressing saccharomyces cerevisiae on duodenal development in weaned piglets","volume":"115","author":"Wang","year":"2015","journal-title":"Brit. J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S41","DOI":"10.1016\/j.jaci.2009.09.046","article-title":"Structure and function of immunoglobulins","volume":"125","author":"Schroeder","year":"2010","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1643e50","DOI":"10.1002\/jsfa.9348","article-title":"Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs","volume":"99","author":"Zou","year":"2019","journal-title":"J. Sci. Food Agric."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.2741\/4669","article-title":"Glucose and amino acid in enterocyte: Absorption, metabolism and maturation","volume":"23","author":"Chen","year":"2018","journal-title":"Front. Biosci. (Landmark Ed)"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Chen, L., Tuo, B., and Dong, H. (2016). Regulation of Intestinal Glucose Absorption by Ion Channels and Transporters. Nutrients, 8.","DOI":"10.3390\/nu8010043"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.molmet.2016.10.008","article-title":"Intestinal invalidation of the glucose transporter GLUT2 delays tissue distribution of glucose and reveals an unexpected role in gut homeostasis","volume":"6","author":"Schmitt","year":"2016","journal-title":"Mol. Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1007\/s00424-020-02439-5","article-title":"Glucose transporters in the small intestine in health and disease","volume":"472","author":"Koepsell","year":"2020","journal-title":"Pflugers Arch."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.clinbiochem.2010.07.022","article-title":"Malondialdehyde (MDA) and protein carbonyl (PCO) levels as biomarkers of oxidative stress in subjects with familial hypercholesterolemia","volume":"43","author":"Pirinccioglu","year":"2010","journal-title":"Clin. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Yan, E., Zhang, J., Han, H., Wu, J., Gan, Z., Wei, C., Zhang, L., Wang, C., and Wang, T. (2019). Curcumin Alleviates IUGR Jejunum Damage by Increasing Antioxidant Capacity through Nrf2\/Keap1 Pathway in Growing Pigs. Animals, 10.","DOI":"10.3390\/ani10010041"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/jam.12833","article-title":"Oral administration of Lactococcus lactis-expressed recombinant porcine epidermal growth factor (rpEGF) stimulates the development and promotes the health of small intestines in early-weaned piglets","volume":"119","author":"Xu","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"G938","DOI":"10.1152\/ajpgi.00090.2006","article-title":"Intestinal barrier failure during experimental necrotizing enterocolitis: Protective effect of EGF treatment","volume":"291","author":"Clark","year":"2006","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1136\/gutjnl-2020-322260","article-title":"Mucus barrier, mucins and gut microbiota: The expected slimy partners?","volume":"69","author":"Paone","year":"2020","journal-title":"Gut"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12182","article-title":"The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system","volume":"260","author":"Pelaseyed","year":"2014","journal-title":"Immunol. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jbiotec.2015.01.007","article-title":"Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved","volume":"196\u2013197","author":"Bedford","year":"2015","journal-title":"J. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Schoultz, I., and Keita, \u00c5.V. (2020). The Intestinal Barrier and Current Techniques for the Assessment of Gut Permeability. Cells, 9.","DOI":"10.3390\/cells9081909"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/17\/2245\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:35Z","timestamp":1760142035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/17\/2245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":48,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ani12172245"],"URL":"https:\/\/doi.org\/10.3390\/ani12172245","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}