{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:00:17Z","timestamp":1790974817175,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Background: We tested the hypothesis that the ratio between lactate dehydrogenase activity (LDH) and total nucleated cell counts (TNCC) in effusions may be useful to diagnose feline infectious peritonitis (FIP). Methods: LDH\/TNCC ratio was retrospectively evaluated in 648 effusions grouped based on cytology and physicochemical analysis (step 1), on the probability of FIP estimated by additional tests on fluids (step 2) or on other biological samples (step 3, n = 471). Results of different steps were statistically compared. Receiver Operating Characteristic (ROC) curves were designed to assess whether the ratio identify the samples with FIP \u201cprobable\/almost confirmed\u201d. The cut-offs with the highest positive likelihood ratio (LR+) or Youden Index (YI) or with equal sensitivity and specificity were determined. Results: A high median LDH\/TNCC ratio was found in FIP effusions (step1: 2.01) and with probable or almost confirmed FIP (step 2: 1.99; 2.20 respectively; step 3: 1.26; 2.30 respectively). The optimal cut-offs were 7.54 (LR+ 6.58), 0.62 (IY 0.67, sensitivity: 89.1%; specificity 77.7%), 0.72 (sensitivity and specificity: 79.2%) in step 2 and 2.27 (LR+ 10.39), 0.62 (IY 0.65, sensitivity: 82.1%; specificity 83.0%), 0.54 (sensitivity: 82.1%; specificity 81.9%) in step 3. Conclusions: a high LDH\/TNCC ratio support a FIP diagnosis.<\/jats:p>","DOI":"10.3390\/ani12172262","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T23:53:21Z","timestamp":1661990001000},"page":"2262","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH\/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7310-0641","authenticated-orcid":false,"given":"Pierpaolo","family":"Romanelli","sequence":"first","affiliation":[{"name":"MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7117-7987","authenticated-orcid":false,"given":"Saverio","family":"Paltrinieri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugo","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Graziano","family":"Castaman","sequence":"additional","affiliation":[{"name":"MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Monza","sequence":"additional","affiliation":[{"name":"MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Bertazzolo","sequence":"additional","affiliation":[{"name":"MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1177\/1098612X18758592","article-title":"Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests","volume":"20","author":"Tasker","year":"2018","journal-title":"J. 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