{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:29:11Z","timestamp":1791613751700,"version":"4.3.4"},"reference-count":60,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research &amp; Development Plan","award":["2021YFD1200403"],"award-info":[{"award-number":["2021YFD1200403"]}]},{"name":"National Key Research &amp; Development Plan","award":["32060753"],"award-info":[{"award-number":["32060753"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021YFD1200403"],"award-info":[{"award-number":["2021YFD1200403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060753"],"award-info":[{"award-number":["32060753"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Project of Guizhou Province","award":["2021YFD1200403"],"award-info":[{"award-number":["2021YFD1200403"]}]},{"name":"Science and Technology Project of Guizhou Province","award":["32060753"],"award-info":[{"award-number":["32060753"]}]},{"name":"Guizhou high level Innovative Talents Project","award":["2021YFD1200403"],"award-info":[{"award-number":["2021YFD1200403"]}]},{"name":"Guizhou high level Innovative Talents Project","award":["32060753"],"award-info":[{"award-number":["32060753"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Dietary supplementation with N-acetyl-L-cysteine (NAC) may support early pregnancy regulation and fertility in female animals. The purpose of this study was to investigate the effect of supplementation with 0.07% NAC on the expression of the uterine keratin gene and protein in Qianbei-pockmarked goats during early pregnancy using tandem mass spectrometry (TMT) relative quantitative proteomics. The results showed that there were significant differences in uterine keratin expression between the experimental group (NAC group) and the control group on day 35 of gestation. A total of 6271 proteins were identified, 6258 of which were quantified by mass spectrometry. There were 125 differentially expressed proteins (DEPs), including 47 upregulated and 78 downregulated proteins, in the NAC group. Bioinformatic analysis showed that these DEPs were mainly involved in the transport and biosynthesis of organic matter and were related to the binding of transition metal ions, DNA and proteins and the catalytic activity of enzymes. They were enriched in the Jak-STAT signalling pathway, RNA monitoring pathway, amino acid biosynthesis, steroid biosynthesis and other pathways that may affect the early pregnancy status of does through different pathways and thus influence early embryonic development. Immunohistochemistry, real-time quantitative PCR and Western blotting were used to verify the expression and localization of glial fibrillary acidic protein (GFAP) and pelota mRNA surveillance and ribosomal rescue factor (PELO) in uterine horn tissue. The results showed that both PELO and GFAP were localized to endometrial and stromal cells, consistent with the mass spectrometry data at the transcriptional and translational levels. Moreover, NAC supplementation increased the levels of the reproductive hormones follicle-stimulating hormone (FSH), luteinizing hormone (LH), oestradiol (E2), progesterone (P4), superoxide dismutase (SOD), glutamate peroxidase (GSH-Px) and nitric oxide (NO) in the serum of does. These findings provide new insight into the mechanism by which NAC regulates early pregnancy and embryonic development in goats.<\/jats:p>","DOI":"10.3390\/ani12182439","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T22:11:10Z","timestamp":1663279870000},"page":"2439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Proteomic Study of the Effect of N-acetylcysteine on the Regulation of Early Pregnancy in Goats"],"prefix":"10.3390","volume":"12","author":[{"given":"Peifang","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzhou","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhinan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1161-2657","authenticated-orcid":false,"given":"Kaibin","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3594-6800","authenticated-orcid":false,"given":"Jiafu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, China"},{"name":"College of Animal Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0895-1356","authenticated-orcid":false,"given":"Yong","family":"Ruan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Guiyang 550025, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang 550025, 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Reprod. Fertil. Suppl."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/rda.13647","article-title":"Embryo gene expression in pig pregnancy","volume":"55","author":"Llobat","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1177\/1933719117711258","article-title":"Effect of 2,3\u2032,4,4\u2032,5-Pentachlorobiphenyl Exposure on Endometrial Receptivity and the Methylation of HOXA10","volume":"25","author":"Qu","year":"2017","journal-title":"Reprod. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3878","DOI":"10.1021\/pr100096b","article-title":"Proteomic Profile of Uterine Luminal Fluid from Early Pregnant Ewes","volume":"6","author":"Koch","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/oxfordjournals.humrep.a136378","article-title":"Causes of early embryonic loss in human pregnancy","volume":"3","author":"Edwards","year":"1986","journal-title":"Hum. Reprod."},{"key":"ref_6","first-page":"7","article-title":"Placental Responses to Changes in the Maternal Environment Determine Fetal Growth","volume":"29","author":"Dimasuay","year":"2016","journal-title":"Front. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3004","DOI":"10.1021\/pr300144q","article-title":"Proteomic Characterization of Histotroph during the Preimplantation Phase of the Estrous Cycle in Cattle","volume":"11","author":"Mullen","year":"2012","journal-title":"J. Proteome Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/ppe.12465","article-title":"Association between prenatal psychological stress and oxidative stress during pregnancy","volume":"32","author":"Eick","year":"2018","journal-title":"Paediatr. Pe\u00e9rinat. Epidemiology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e12868","DOI":"10.1111\/aji.12868","article-title":"Saturated and unsaturated fatty acids differentially regulate in vitro and ex vivo placental antioxidant capacity","volume":"80","author":"Manuel","year":"2018","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1177\/1933719117702247","article-title":"Choline Supplementation During Pregnancy Protects Against Gestational Lipopolysaccharide-Induced Inflammatory Responses","volume":"25","author":"Zhang","year":"2018","journal-title":"Reprod. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.anifeedsci.2016.04.012","article-title":"Effect of feeding diets with processed Moringa oleifera meal as protein source in lactating Anglo-Nubian goats","volume":"217","author":"Kholif","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.sajb.2019.03.022","article-title":"Antioxidant potential of Moringa leaves for improvement of milk and serum quality of Aardi goats","volume":"129","author":"Alsawmahi","year":"2020","journal-title":"S. Afr. J. Bot."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"566","DOI":"10.5455\/javar.2020.g454","article-title":"Effect of prepartum dietary energy source on goat maternal metabolic profile, neonatal performance, and economic profitability","volume":"7","author":"Mahboub","year":"2020","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1007\/s00784-020-03721-7","article-title":"Effects of N-acetyl cysteine on mitochondrial ROS, mitochondrial dynamics, and inflammation on lipopolysaccharide-treated human apical papilla cells","volume":"25","author":"Jariyamana","year":"2021","journal-title":"Clin. Oral Investig."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tian, X., Li, J., Luo, Q., Wang, X., Wang, T., Zhou, D., Xie, L., Ban, C., and Lu, Q. (2022). Effects of Purple Corn Anthocyanin on Growth Performance, Meat Quality, Muscle Antioxidant Status, and Fatty Acid Profiles in Goats. Foods, 11.","DOI":"10.3390\/foods11091255"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1093\/carcin\/22.7.999","article-title":"Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points","volume":"22","author":"Izzotti","year":"2001","journal-title":"Carcinogenesis"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.pharmthera.2013.09.006","article-title":"Existing and potential therapeutic uses for N-acetylcysteine: The need for conversion to intracellular glutathione for antioxidant benefits","volume":"141","author":"Rushworth","year":"2014","journal-title":"Pharmacol. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/s41467-020-14788-x","article-title":"Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection","volume":"11","author":"Wei","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1002\/tox.22221","article-title":"N-acetyl cysteine-mediated effective attenuation of methoxychlor-induced granulosa cell apoptosis by counteracting reactive oxygen species generation in caprine ovary","volume":"32","author":"Bhardwaj","year":"2017","journal-title":"Environ. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s10815-018-1215-4","article-title":"Preincubation with glutathione ethyl ester improves the developmental competence of vitrified mouse oocytes","volume":"35","author":"Li","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1093\/humrep\/des019","article-title":"Delay in oocyte aging in mice by the antioxidant N-acetyl-l-cysteine (NAC)","volume":"27","author":"Liu","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.cryobiol.2016.05.002","article-title":"N-acetyl cysteine prolonged the developmental ability of mouse two-cell embryos against oxidative stress at refrigerated temperatures","volume":"72","author":"Horikoshi","year":"2016","journal-title":"Cryobiology"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Guo, M.-Y., Wang, H., Chen, Y.-H., Xia, M.-Z., Zhang, C., and Xu, D.-X. (2018). N-acetylcysteine alleviates cadmium-induced placental endoplasmic reticulum stress and fetal growth restriction in mice. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191667"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.theriogenology.2012.05.013","article-title":"Effects of oral treatment with N-acetylcysteine on the viscosity of intrauterine mucus and endometrial function in estrous mares","volume":"78","author":"Witte","year":"2012","journal-title":"Theriogenology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.18632\/aging.101898","article-title":"N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse","volume":"11","author":"Wang","year":"2019","journal-title":"Aging"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e22046","DOI":"10.1002\/jbt.22046","article-title":"N-Acetyl-cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue","volume":"32","author":"Bhardwaj","year":"2018","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1002\/vms3.338","article-title":"Effects of N-Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats","volume":"7","author":"Luo","year":"2020","journal-title":"Vet. Med. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1021\/ac0262560","article-title":"Tandem Mass Tags: A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS\/MS","volume":"75","author":"Andrew","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.yexcr.2019.02.016","article-title":"Proteomic analysis of mouse ovaries during the prepubertal stages","volume":"377","author":"Xiong","year":"2019","journal-title":"Exp. Cell Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e1800003","DOI":"10.1002\/pmic.201800003","article-title":"Quantitative Analysis of the Global Proteome in Peripheral Blood Mononuclear Cells from Patients with New-Onset Psoriasis","volume":"18","author":"Li","year":"2018","journal-title":"PROTEOMICS"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.cell.2016.05.069","article-title":"CPTAC Investigators. Integrated Proteogenomic Characterization of Human High-Grade Serous Ovarian Cancer","volume":"166","author":"Zhang","year":"2016","journal-title":"Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"105","DOI":"10.5194\/aab-65-105-2022","article-title":"TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells","volume":"65","author":"Hong","year":"2022","journal-title":"Arch. Anim. Breed."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.domaniend.2016.05.006","article-title":"Molecular cloning, expression analysis, and function of decorin in goat ovarian granulosa cells","volume":"57","author":"Peng","year":"2016","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1631\/jzus.B2100366","article-title":"Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats","volume":"22","author":"Zhou","year":"2021","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"792869","DOI":"10.3389\/fvets.2021.792869","article-title":"Proteomic Exploration of Porcine Oocytes During Meiotic Maturation in vitro Using an Accurate TMT-Based Quantitative Approach","volume":"8","author":"Jia","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_36","first-page":"1018","article-title":"[Progress in proteomics of mammalian oocyte and early embryo]","volume":"30","author":"Chen","year":"2014","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/humupd\/dmy008","article-title":"Trophoblast retrieval and isolation from the cervix: Origins of cervical trophoblasts and their potential value for risk assessment of ongoing pregnancies","volume":"24","author":"Moser","year":"2018","journal-title":"Hum. Reprod. Updat."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.theriogenology.2020.11.005","article-title":"Influence of N-acetylcysteine on steroidogenesis and gene expression in porcine placental trophoblast cells","volume":"161","author":"Ding","year":"2021","journal-title":"Theriogenology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1113\/expphysiol.1989.sp003316","article-title":"Uptake of Amino Acids and Ammonia at Mid-Gestation by the Fetal Lamb.","volume":"74","author":"Bell","year":"1989","journal-title":"Q. J. Exp. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Tian, X., Lu, Q., Zhao, S., Li, J., Luo, Q., Wang, X., Zhang, Y., and Zheng, N. (2021). Purple Corn Anthocyanin Affects Lipid Mechanism, Flavor Compound Profiles, and Related Gene Expression of Longissimus Thoracis et Lumborum Muscle in Goats. Animals, 11.","DOI":"10.3390\/ani11082407"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"813672","DOI":"10.3389\/fvets.2021.813672","article-title":"Effect of Supplementation with Selenium-Yeast on Muscle Antioxidant Activity, Meat Quality, Fatty Acids and Amino Acids in Goats","volume":"8","author":"Tian","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_42","first-page":"1757","article-title":"Effects of n-acetyl-cysteine supplementation in late gestational diet on maternal-placental redox status, placental NLRP3 inflammasome, and fecal microbiota in sows1","volume":"97","author":"Luo","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1186\/s13287-018-0913-2","article-title":"Neural stem cell therapy for subacute and chronic ischemic stroke","volume":"9","author":"Boese","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1002\/glia.22990","article-title":"Gfap-positive radial glial cells are an essential progenitor population for later-born neurons and glia in the zebrafish spinal cord","volume":"64","author":"Johnson","year":"2016","journal-title":"Glia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1159\/000085980","article-title":"Morphogenesis of the Dentate Gyrus: What We Are Learning from Mouse Mutants","volume":"27","author":"Li","year":"2005","journal-title":"Dev. Neurosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1111\/febs.15643","article-title":"Perturbed differentiation of murine embryonic stem cells upon Pelota deletion due to dysregulated FOXO1\/\u03b2-catenin signaling","volume":"288","author":"Elkenani","year":"2021","journal-title":"FEBS J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1038\/onc.2013.35","article-title":"PELO negatively regulates HER receptor signalling and metastasis","volume":"33","author":"Pedersen","year":"2014","journal-title":"Oncogene"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/genetics\/149.1.45","article-title":"Yeast dom34 Mutants Are Defective in Multiple Developmental Pathways and Exhibit Decreased Levels of Polyribosomes","volume":"149","author":"Davis","year":"1998","journal-title":"Genetics"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3477","DOI":"10.1242\/dev.121.10.3477","article-title":"The pelota locus encodes a protein required for meiotic cell division: An analysis of G2\/M arrest in Drosophila spermatogenesis","volume":"121","author":"Eberhart","year":"1995","journal-title":"Development"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0015-0282(16)58201-6","article-title":"Ovarian response to recombinant human follicle-stimulating hormone in luteinizing hormone-depleted women: Examination of the two cell, two gonadotropin theory","volume":"65","author":"Gotlieb","year":"1996","journal-title":"Fertil. Steril."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1071\/RD20148","article-title":"Effects of LH and FSH on androgen and oestrogen release in the myometrium of pigs during the oestrous cycle and early pregnancy","volume":"32","author":"Waszkiewicz","year":"2020","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4033","DOI":"10.1210\/en.2018-00497","article-title":"FSH Actions and Pregnancy: Looking Beyond Ovarian FSH Receptors","volume":"159","author":"Stilley","year":"2018","journal-title":"Endocrinology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1262\/jrd.09-179T","article-title":"Ovarian Follicular Dynamics and Hormonal Changes in Goats during Early Pregnancy","volume":"56","author":"Kandiel","year":"2010","journal-title":"J. Reprod. Dev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1017\/S1751731119002982","article-title":"Supplemental progesterone during early pregnancy exerts divergent responses on embryonic characteristics in sows and gilts","volume":"14","author":"Muro","year":"2020","journal-title":"Animal"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.domaniend.2018.12.008","article-title":"The effect of estrone and estradiol on the expression of the orexin\/hypocretin system in the porcine uterus during early pregnancy","volume":"68","author":"Kiezun","year":"2019","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"42024","DOI":"10.1007\/s11356-021-13720-0","article-title":"Exposure to butylated hydroxytoluene compromises endometrial decidualization during early pregnancy","volume":"28","author":"Sun","year":"2021","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/0006-2952(94)90528-2","article-title":"Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells","volume":"47","author":"Lu","year":"1994","journal-title":"Biochem. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jnutbio.2007.02.005","article-title":"Selenium as an anticancer nutrient: Roles in cell proliferation and tumor cell invasion","volume":"19","author":"Zeng","year":"2008","journal-title":"J. Nutr. Biochem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"7880","DOI":"10.18632\/aging.102295","article-title":"Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa","volume":"11","author":"Liu","year":"2019","journal-title":"Aging"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Shao, Y., Chen, Z., and Wu, L. (2019). Oxidative Stress Effects of Soluble Sulfide on Human Hepatocyte Cell Line LO2. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16091662"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/18\/2439\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:32:25Z","timestamp":1760142745000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/18\/2439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,15]]},"references-count":60,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ani12182439"],"URL":"https:\/\/doi.org\/10.3390\/ani12182439","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,15]]}}}