{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:21:29Z","timestamp":1790709689221,"version":"4.1.0"},"reference-count":97,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Economy and Competitiveness","doi-asserted-by":"publisher","award":["RTA08-099"],"award-info":[{"award-number":["RTA08-099"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Economy and Competitiveness","doi-asserted-by":"publisher","award":["RTA2008-00080-C02"],"award-info":[{"award-number":["RTA2008-00080-C02"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Economy and Competitiveness","doi-asserted-by":"publisher","award":["RTA2014-00045-C03-01"],"award-info":[{"award-number":["RTA2014-00045-C03-01"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Economy and Competitiveness","doi-asserted-by":"publisher","award":["RTI2018-098267-R-C31"],"award-info":[{"award-number":["RTI2018-098267-R-C31"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["RTA08-099"],"award-info":[{"award-number":["RTA08-099"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["RTA2008-00080-C02"],"award-info":[{"award-number":["RTA2008-00080-C02"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["RTA2014-00045-C03-01"],"award-info":[{"award-number":["RTA2014-00045-C03-01"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["RTI2018-098267-R-C31"],"award-info":[{"award-number":["RTI2018-098267-R-C31"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Listeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. Here we show the prevalence and transmission of L. monocytogenes in dairy farms in the Cantabria region, on the northern coast of Spain. A total of 424 samples was collected from 14 dairy farms (5 organic and 9 conventional) and 211 L. monocytogenes isolates were recovered following conventional microbiological methods. There were no statistically significant differences in antimicrobial resistance ratios between organic and conventional farms. A clonal relationship among the isolates was assessed by pulsed field gel electrophoresis (PFGE) analysis and 64 different pulsotypes were obtained. Most isolates (89%, n = 187) were classified as PCR serogroup IVb by using a multiplex PCR assay. In this case, 45 isolates of PCR serogroup IVb were whole genome-sequenced to perform a further analysis at genomic level. In silico MLST analysis showed the presence of 12 sequence types (ST), of which ST1, ST54 and ST666 were the most common. Our data indicate that the environment of cattle farms retains a high incidence of L. monocytogenes, including subtypes involved in human listeriosis reports and outbreaks. This pathogen is shed in the feces and could easily colonize dairy products, as a result of fecal contamination. Effective herd and manure management are needed in order to prevent possible outbreaks.<\/jats:p>","DOI":"10.3390\/ani12182477","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T21:47:27Z","timestamp":1663624047000},"page":"2477","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6739-8859","authenticated-orcid":false,"given":"Athanasia","family":"Varsaki","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n y Formaci\u00f3n Agrarias (CIFA), 39600 Muriedas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagrario","family":"Ortiz","sequence":"additional","affiliation":[{"name":"National Institute for Agricultural and Food Research and Technology (INIA)-Spanish National Research Council (CSIC), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Santorum","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Formaci\u00f3n Agrarias (CIFA), 39600 Muriedas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"National Institute for Agricultural and Food Research and Technology (INIA)-Spanish National Research Council (CSIC), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8129-8352","authenticated-orcid":false,"given":"Victoria","family":"L\u00f3pez-Alonso","sequence":"additional","affiliation":[{"name":"National Institute of Health Carlos III, 28222 Majadahonda, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6914-9987","authenticated-orcid":false,"given":"Marta","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n (ITACyL), 47071 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Abad","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n (ITACyL), 47071 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-6049","authenticated-orcid":false,"given":"Jorge","family":"Rodr\u00edguez-Grande","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla, Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain A.","family":"Ocampo-Sosa","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla, Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn V.","family":"Mart\u00ednez-Su\u00e1rez","sequence":"additional","affiliation":[{"name":"National Institute for Agricultural and Food Research and Technology (INIA)-Spanish National Research Council (CSIC), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1007\/s00018-003-2225-6","article-title":"Pathogen, Host and Environmental Factors Contributing to the Pathogenesis of Listeriosis","volume":"60","author":"Roberts","year":"2003","journal-title":"Cell. 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