{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:33:46Z","timestamp":1782952426741,"version":"3.54.5"},"reference-count":43,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>A 15-day-old, female, captive Panthera tigris cub was hospitalized after developing severe hyperthermia, depression, and lack of appetite. The clinical condition rapidly worsened, and the tiger cub died in 72 h after the onset of neurological symptoms, septic shock, and multiple organ dysfunction syndrome. The postmortem main gross findings consisted of a severe and diffuse bilateral fibrino-suppurative meningoencephalitis and ventriculitis, mild fibrinous and sero-hemorrhagic polyserositis and cystitis, severe pulmonary edema, and hemorrhages. Microscopically, the meninges, ependyma, and choroid plexuses were diffusely expanded by abundant infiltration of neutrophils and macrophages, with multifocal fibrinous exudation. Histiocytic interstitial pneumonia, fibrinous and neutrophilic polyserositis, and pyelocystitis were also observed. Vascular thrombosis with multifocal vasculitis and vascular necrosis were frequently observed. Aerobic and anaerobic cultures performed on the brain, lungs, intestine, kidneys, and in pericardial effusion reported the presence of Salmonella enterica subsp. enterica serovar Enteritidis. Environmental and nutritional contamination were identified as putative sources of infections. To the best of the authors\u2019 knowledge, this is the first report of Salmonella Enteritidis septicemia with meningoencephalitis in a tiger cub, which highlights the need to further investigate the cause of acute perinatal death to reduce the risk of infectious disease outbreaks.<\/jats:p>","DOI":"10.3390\/ani12192490","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T21:12:53Z","timestamp":1663708373000},"page":"2490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Salmonella Enteritidis Fatal Septicemia with Meningoencephalitis in a Tiger (Panthera tigris) Cub"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-8467","authenticated-orcid":false,"given":"Elisa","family":"Mazzotta","sequence":"first","affiliation":[{"name":"Diagnostics in Animal Health Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8400-8573","authenticated-orcid":false,"given":"Greta","family":"Foiani","sequence":"additional","affiliation":[{"name":"Specialist Diagnostics, Histopathology and Parasitology Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8422-6829","authenticated-orcid":false,"given":"Giulia Maria","family":"De Benedictis","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Productions and Health (MAPS), University of Padua, Viale dell\u2019Universit\u00e0 12, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-9553","authenticated-orcid":false,"given":"Enrico","family":"Fiore","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Productions and Health (MAPS), University of Padua, Viale dell\u2019Universit\u00e0 12, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2104-4531","authenticated-orcid":false,"given":"Alda","family":"Natale","sequence":"additional","affiliation":[{"name":"Diagnostics in Animal Health Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Spagnolo","sequence":"additional","affiliation":[{"name":"Diagnostics in Animal Health Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4537-4386","authenticated-orcid":false,"given":"Marta","family":"Vascellari","sequence":"additional","affiliation":[{"name":"Specialist Diagnostics, Histopathology and Parasitology Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5846-7231","authenticated-orcid":false,"given":"Giulia","family":"Cento","sequence":"additional","affiliation":[{"name":"WOAH and National Reference Laboratory for Salmonellosis, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Corr\u00f2","sequence":"additional","affiliation":[{"name":"Diagnostics in Animal Health Department, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0 10, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"ref_1","unstructured":"Grimont, P.A.D., and Weil, F. (2007). WHO Collaborating Centre for Reference and Research on Salmonella. Antigenic Formulae of the Salmonella Serovars, Institute Pasteur. [9th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.resmic.2014.07.004","article-title":"Supplement 2008\u20132010 (no. 48) to the White\u2013Kauffmann\u2013Le Minor scheme","volume":"165","author":"Roggentin","year":"2014","journal-title":"Res. Microbiol."},{"key":"ref_3","unstructured":"Maxie, M.G. (2016). Alimentary System. Jubb, Kennedy & Palmer\u2019s Pathology of Domestic Animals, Elsevier."},{"key":"ref_4","unstructured":"Zachary, J.F., and McGavin, M.D. (2011). Alimentary System and the Peritoneum, Omentum, Mesentery, and Peritoneal Cavity. Pathologic Basis of Veterinary Disease, Mosby."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1638\/1042-7260(2002)033[0083:PEODCO]2.0.CO;2","article-title":"Positive effects of diet change on shedding of Salmonella spp. in the feces of captive felids","volume":"33","author":"Lewis","year":"2002","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_6","first-page":"76","article-title":"A Molecular Epidemiologic Investigation of Salmonella from a Meat Source to the Feces of Captive Cheetah (Acinonyx Jubatus)","volume":"34","author":"Venter","year":"2003","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101792","DOI":"10.1016\/j.cimid.2022.101792","article-title":"Salmonella enterica subsp. enterica serotypes isolated for the first time in feral cats: The impact on public health","volume":"84","author":"Rosario","year":"2022","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"263","DOI":"10.2478\/jvetres-2020-0032","article-title":"Prevalence and drug resistance of Salmonella in dogs and cats in Xuzhou, China","volume":"64","author":"Wei","year":"2020","journal-title":"J. Veter. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4142\/jvs.2017.18.3.273","article-title":"Phenotypic and genotypic antimicrobial resistance and virulence genes of Salmonella enterica isolated from pet dogs and cats","volume":"18","author":"Srisanga","year":"2017","journal-title":"J. Veter. Sci."},{"key":"ref_10","first-page":"148","article-title":"Fecal Shedding of Salmonella in Exotic Felids","volume":"28","author":"Clyde","year":"1997","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2527\/tas2017.0047","article-title":"Evaluation of raw pork as a commercially manufactured diet option for zoo-managed African wildcats (Felis silvestris lybica)","volume":"1","author":"Iske","year":"2017","journal-title":"Transl. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"538","DOI":"10.5326\/0390538","article-title":"Septicemic Salmonellosis in Two Cats Fed a Raw-Meat Diet","volume":"39","author":"Stiver","year":"2003","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1128\/microbiolspec.OH-0020-2013","article-title":"One Health and Food-Borne Disease: Salmonella Transmission between Humans, Animals, and Plants","volume":"2","author":"Silva","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.3855\/jidc.5393","article-title":"Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa","volume":"8","author":"Smith","year":"2014","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Proverbio, D., Perego, R., Baggiani, L., Ravasio, G., Giambellini, D., and Spada, E. (2021). Hematological and Biochemical Reference Values in Healthy Captive Tigers (Panthera tigris). Animals, 11.","DOI":"10.3390\/ani11123440"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1638\/2020-0132","article-title":"Mortality review for the North American snow leopard (Panthera uncia) zoo population from January 1999 to December 2019","volume":"52","author":"Womble","year":"2021","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1638\/2019-0206","article-title":"Retrospective study of causes of animal mortality in Ljubljana Zoo 2005\u20132015","volume":"51","author":"Cigler","year":"2020","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Junginger, J., Hansmann, F., Herder, V., Lehmbecker, A., Peters, M., Beyerbach, M., Wohlsein, P., and Baumg\u00e4rtner, W. (2015). Pathology in Captive Wild Felids at German Zoological Gardens. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0130573"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jviromet.2008.05.018","article-title":"Development of a quantitative real-time TaqMan PCR assay for testing the susceptibility of feline herpesvirus-1 to antiviral compounds","volume":"152","author":"Hussein","year":"2008","journal-title":"J. Virol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jviromet.2006.05.004","article-title":"Detection of canine distemper virus in dogs by real-time RT-PCR","volume":"136","author":"Elia","year":"2006","journal-title":"J. Virol. Methods"},{"key":"ref_21","first-page":"25","article-title":"Diagnostica Della Neosporosi Bovina Nel Nord Italia","volume":"3","author":"Magnino","year":"2000","journal-title":"Large Anim. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1099\/0022-1317-82-7-1589","article-title":"Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats","volume":"82","author":"Huder","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jviromet.2017.10.001","article-title":"Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation","volume":"251","author":"Meli","year":"2018","journal-title":"J. Virol. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vetmic.2004.09.018","article-title":"A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs","volume":"105","author":"Decaro","year":"2005","journal-title":"Veter. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1053\/j.tcam.2012.07.002","article-title":"Diseases Associated with Thrombosis","volume":"27","year":"2012","journal-title":"Top. Companion Anim. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ajpath.2016.09.002","article-title":"Salmonella Meningitis Associated with Monocyte Infiltration in Mice","volume":"187","author":"Bauler","year":"2016","journal-title":"Am. J. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1128\/iai.5.2.191-198.1972","article-title":"Salmonellosis in orally infected specific pathogen-free C57B1 mice","volume":"5","author":"Collins","year":"1972","journal-title":"Infect Immun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"624622","DOI":"10.3389\/fcimb.2020.624622","article-title":"Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract","volume":"10","author":"Harrell","year":"2021","journal-title":"Front. Cell. Infect. Microbiol"},{"key":"ref_29","first-page":"133","article-title":"Molecular Mechanisms Used by Salmonella to Evade the Immune System","volume":"25","year":"2018","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.anireprosci.2013.11.006","article-title":"Factors influencing breeding success, ovarian cyclicity, and cub survival in zoo-managed tigers (Panthera tigris)","volume":"144","author":"Saunders","year":"2014","journal-title":"Anim. Reprod. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hampson, M.C., and Schwitzer, C. (2016). Effects of Hand-Rearing on Reproductive Success in Captive Large Cats Panthera tigris altaica, Uncia uncia, Acinonyx jubatus and Neofelis nebulosa. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0155992"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1177\/104063871002200130","article-title":"Necrotizing Pneumonia and Pleuritis Associated with Extraintestinal Pathogenic Escherichia Coli in a Tiger (Panthera tigris) Cub","volume":"22","author":"Carvallo","year":"2010","journal-title":"J. Veter. Diagn. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1638\/2008-0015.1","article-title":"Fatal Infection with Feline Panleukopenia Virus in Two Captive Wild Carnivores (Panthera tigris and Panthera leo)","volume":"40","author":"Duarte","year":"2009","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"594","DOI":"10.7589\/0090-3558-37.3.594","article-title":"Parvovirus Infections in Wild Carnivores","volume":"37","author":"Steinel","year":"2001","journal-title":"J. Wildl. Dis."},{"key":"ref_35","first-page":"285","article-title":"Prevalence of Salmonella Spp. in Captive Wildlife at the National Zoological Garden Jos, Nigeria","volume":"1","author":"Oladapo","year":"2013","journal-title":"Curr. Res. Microbiol. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/jsap.13000","article-title":"Raw diets for dogs and cats: A review, with particular reference to microbiological hazards","volume":"60","author":"Davies","year":"2019","journal-title":"J. Small Anim. Pract."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"525","DOI":"10.2460\/javma.247.5.525","article-title":"Salmonella bacteriuria in a cat fed a Salmonella-contaminated diet","volume":"247","author":"Fauth","year":"2015","journal-title":"J. Am. Veter. Med. Assoc."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Andruzzi, M.N., Krath, M.L., Lawhon, S.D., and Boudreau, B. (2020). Salmonella enterica subspecies houtenae as an opportunistic pathogen in a case of meningoencephalomyelitis and bacteriuria in a dog. BMC Veter. Res., 16.","DOI":"10.1186\/s12917-020-02652-5"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1297-9716-42-34","article-title":"Animal contact as a source of human non-typhoidal salmonellosis","volume":"42","author":"Hoelzer","year":"2011","journal-title":"Veter. Res."},{"key":"ref_40","first-page":"1975530","article-title":"Zoonotic potential and prevalence of Salmonella serovars isolated from pets","volume":"11","author":"Paluch","year":"2021","journal-title":"Infect. Ecol. Epidemiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"702725","DOI":"10.3389\/fmicb.2021.702725","article-title":"Heavy Metal Resistance in Salmonella Typhimurium and Its Association with Disinfectant and Antibiotic Resistance","volume":"12","author":"Mustafa","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/zoo.20359","article-title":"Human-Animal Bonds Between Zoo Professionals and the Animals in Their Care","volume":"31","author":"Hosey","year":"2012","journal-title":"Zoo Biol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Rahman, M.M., Alam Tumpa, M.A., Zehravi, M., Sarker, T., Islam, R., Rashid, H.O., Ahmed, M., Ramproshad, S., and Mondal, B. (2022). An Overview of Antimicrobial Stewardship Optimization: The Use of Antibiotics in Humans and Animals to Prevent Resistance. Antibiotics, 11.","DOI":"10.3390\/antibiotics11050667"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/19\/2490\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:34:58Z","timestamp":1760142898000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/19\/2490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,20]]},"references-count":43,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ani12192490"],"URL":"https:\/\/doi.org\/10.3390\/ani12192490","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,20]]}}}