{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:10:36Z","timestamp":1790349036735,"version":"4.1.0"},"reference-count":31,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The main purpose of the study was to analyse the concentrations of selected essential and toxic trace elements in the hair of sports and recreational riding horses from studs located in central Poland and thus test the usefulness of this matrix for monitoring the exposure of these animals. We also measured the concentration of heavy metals in oats used as a basic component of the equine diet to investigate the interactions between these elements in the feed and hair. The basic chemical composition of oats was analysed. Elemental analysis was performed using an EcaFlow 150 GLP electrochemical analyser with an E-104L electrode and reference to a calibration curve. The chemical composition of oats was investigated by near infrared transmission (NIR) spectroscopy calibrated for an artificial neural network (ANN) using a Foss InfraXact spectrometer. Among all elements, the coefficient of variation was highest for Pb and lowest for Cd, regardless of the study group. The content of elements in horse hair was in the range of 153.56 to 185.79 mg\u00b7kg\u22121 for Zn, 6.10 to 11.99 mg\u00b7kg\u22121 for Cu, 0.578 to 0.813 mg\u00b7kg\u22121 for Pb and 0.011 to 0.015 mg\u00b7kg\u22121 for Cd (in kg of d.w.). For hair, we found a significant negative correlation for Zn-Cu (rxy = \u22120.539) and Cd-Cu (rxy = \u22120.676) at p \u2264 0.05. For feed, there was a highly significant positive correlation for Cu-Pb (rxy = 0.723) and Zn-Cd (rxy = 0.714) at p \u2264 0.01. We found significant negative oats\u2013hair interactions for Cu-Zn, Pb-Cu, Cd-Cu and Zn-Pb, and a positive oats\u2013hair interaction for Cu-Cu.<\/jats:p>","DOI":"10.3390\/ani12192665","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T22:47:27Z","timestamp":1665182847000},"page":"2665","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Concentration of Selected Essential and Toxic Trace Elements in Horse Hair as an Important Tool for the Monitoring of Animal Exposure and Health"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4304-7648","authenticated-orcid":false,"given":"Dorota","family":"Cygan-Szczegielniak","sequence":"first","affiliation":[{"name":"Department of Animal Physiology and Physiotherapy, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6700-141X","authenticated-orcid":false,"given":"Karolina","family":"Stasiak","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Physiotherapy, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e04691","DOI":"10.1016\/j.heliyon.2020.e04691","article-title":"Heavy metal pollution in the environment and their toxicological effects on humans","volume":"6","author":"Briffa","year":"2020","journal-title":"Heliyon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10782","DOI":"10.1007\/s11356-021-16425-6","article-title":"Effects of age and sex on the content of heavy metals in the hair, liver and the longissimus lumborum muscle of roe deer Capreolus capreolus L","volume":"29","author":"Stasiak","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Cygan-Szczegielniak, D. (2021). The Levels of Mineral Elements and Toxic Metals in the Longissimus lumborum Muscle, Hair and Selected Organs of Red Deer (Cervus elaphus L.) in Poland. Animals, 11.","DOI":"10.3390\/ani11051231"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3078","DOI":"10.1016\/j.envpol.2009.05.048","article-title":"Food chain transfer of cadmium and lead to cattle in a lead\u2013zinc smelter in Guizhou, China","volume":"157","author":"Cai","year":"2009","journal-title":"Environ. Pollut."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105192","DOI":"10.1016\/j.envint.2019.105192","article-title":"Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures","volume":"133","author":"Zhou","year":"2019","journal-title":"Environ. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jtemb.2019.05.003","article-title":"Heavy metal toxicity: An update of chelating therapeutic strategies","volume":"54","author":"Kim","year":"2019","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"18554","DOI":"10.1007\/s11356-019-05163-5","article-title":"The content of toxic elements in hair of dairy cows as an indicator of productivity and elemental status of animals","volume":"26","author":"Miroshnikov","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111197","DOI":"10.1016\/j.molliq.2019.111197","article-title":"A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives","volume":"290","author":"Vardhan","year":"2019","journal-title":"J. Mol. Liq."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108901","DOI":"10.1016\/j.jnutbio.2021.108901","article-title":"Zinc is an essential element for the maintenance of redox homeostasis and cell cycle in murine auditory hair cells","volume":"100","author":"Yi","year":"2022","journal-title":"J. Nutr. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0034-5288(03)00074-2","article-title":"Trace element, toxin and drug elimination in hair with particular reference to the horse","volume":"75","author":"Dunnett","year":"2003","journal-title":"Res. Vet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jevs.2015.01.018","article-title":"Effects of dietary mineral intake on hair and serum mineral contents of horses","volume":"35","author":"Ghorbani","year":"2015","journal-title":"J. Equine. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2017.09.017","article-title":"L Elemental hair analysis: A review of procedures and applications","volume":"992","author":"Pozebon","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102922","DOI":"10.1016\/j.jevs.2020.102922","article-title":"Interrelationships between age and trace element concentration in horse mane hair and whole blood","volume":"87","author":"Cassill","year":"2020","journal-title":"J. Equine. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Fazio, F., Gugliandolo, E., Nava, V., Piccione, G., Giannetto, C., and Licata, P. (2020). Bioaccumulation of mineral elements in different biological substrates of athletic horse from Messina, Italy. Animals, 10.","DOI":"10.3390\/ani10101877"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21961","DOI":"10.1007\/s11356-018-2334-2","article-title":"The content of essential and toxic elements in the hair of the mane of the trotter horses depending on their speed","volume":"25","author":"Kalashnikov","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"24620","DOI":"10.1007\/s11356-019-05630-z","article-title":"The total content of toxic elements in horsehair given the level of essential elements","volume":"26","author":"Kalashnikov","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_17","unstructured":"Goszczy\u0144ski, J. (2020). The report on the state of the environment Kuyavian-Pomeranian Province. Voivodeship Inspectorate for Environmental Protection in Bydgoszcz, Library of Environmental, Monitoring. (In Polish)."},{"key":"ref_18","unstructured":"(2014). Polish Standard. Foodstuffs. Determination of Trace Elements. Pressure Digestion. Standard No. PN-EN 13805:2014."},{"key":"ref_19","unstructured":"Ry\u015b, R. (1985). Livestock Feeding Standards, PWRiL. (In Polish)."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1292\/jvms.64.607","article-title":"Concentrations of toxic metals and essential minerals in the many hair of healthy racing horses and their relation to age","volume":"64","author":"Asano","year":"2002","journal-title":"J. Vet. Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jevs.2017.03.133","article-title":"Trace element concentrations in mare and foal mane hair","volume":"52","author":"Hayes","year":"2017","journal-title":"J. Equine Vet. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jevs.2019.03.028","article-title":"Postpartum trace element concentrations in mare and foal mane hair and blood","volume":"76","author":"Krebs","year":"2019","journal-title":"J. Equine Vet. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0048-9697(00)00497-6","article-title":"Concentrations of cadmium, lead and zinc in livestock feed and organs around a metal production centre in eastern Kazakhstan","volume":"257","author":"Farmer","year":"2000","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s12011-017-0991-5","article-title":"The reference intervals of hair trace element content in hereford cows and heifers (Bos taurus)","volume":"180","author":"Miroshnikov","year":"2017","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"103752","DOI":"10.1016\/j.jevs.2021.103752","article-title":"Copper Toxicity in Horses: Does it Exist?","volume":"106","author":"Belli","year":"2021","journal-title":"J. Equine Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1292\/jvms.68.769","article-title":"Relationship between trace elements status in mane hair and atrial fibrillation in horse","volume":"68","author":"Asano","year":"2006","journal-title":"J. Vet. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1023\/A:1026278914222","article-title":"Effect of chelated mineral supplementation on the absorption of Cu, Fe, K, Mn and Zn in horse hair","volume":"258","author":"Armelin","year":"2003","journal-title":"J. Radioanal. Nucl. Chem."},{"key":"ref_28","unstructured":"Weiss, W.P. (2005). National Research Council: Mineral Tolerance of Animals, National Academies Press. [2nd ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1385\/BTER:107:2:135","article-title":"Twenty-eight element concentrations in mane hair samples of adult riding horses determined by particle-induced X-ray emission","volume":"107","author":"Asano","year":"2005","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1385\/BTER:108:1-3:127","article-title":"Correlation between 25 element contents in mane hair in riding horses and atrioventricular block","volume":"108","author":"Asano","year":"2005","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s00244-001-0012-7","article-title":"Interactions between toxic and essential trace metals in cattle from a region with low levels of pollution","volume":"42","author":"Alonso","year":"2002","journal-title":"Arch. Environ. Contam. Toxicol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/19\/2665\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:46:24Z","timestamp":1760143584000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/19\/2665"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,4]]},"references-count":31,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ani12192665"],"URL":"https:\/\/doi.org\/10.3390\/ani12192665","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,4]]}}}