{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:54:04Z","timestamp":1778572444571,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"A.Y.B. from the Minist\u00e8re de l\u2019Enseignement Sup\u00e9rieur et de la Recherche Scientifique d\u2019Alg\u00e9rie","award":["000114"],"award-info":[{"award-number":["000114"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Toxoplasmosis is a zoonotic disease, caused by the protozoan Toxoplasma gondii, affecting most warm-blooded animals. Assessing the seroprevalence of T. gondii in different animal species gives a good estimate of the global circulation of the parasite and the risk for human infections. However, the seroprevalence of T. gondii in dogs is not studied as much as other species, despite their close contact with wildlife and humans in rural or urban environments and evidence that dogs can also be a potential source for human contaminations. A commercial enzyme-inked immunosorbent assay (ELISA) kit to detect anti-T. gondii antibodies in sera of hunting dogs potentially naturally infected, was compared to the modified agglutination test (MAT), used as the reference method. The ELISA presented a sensitivity of 76.5% (CI 95%: 60.0\u201387.6) and a specificity of 87.7% (CI 95%: 76.7\u201393.9) and a substantial agreement with the MAT for the detection of canine anti-T. gondii antibodies. Both tests can therefore be used widely for epidemiology studies on T. gondii infections in dogs. With a mean seroprevalence of T. gondii infection in hunting dogs from northern Algeria of 36.8% (CI 95%: 34.9\u201338.7), this study also highlights the importance of T. gondii seroprevalence studies in companion animals to assess infectious risk for human populations.<\/jats:p>","DOI":"10.3390\/ani12202813","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T21:18:02Z","timestamp":1666127882000},"page":"2813","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of a Commercial Enzyme-Linked Immunosorbent Assay (ELISA) with the Modified Agglutination Test (MAT) for the Detection of Antibodies against Toxoplasma gondii in a Cohort of Hunting Dogs"],"prefix":"10.3390","volume":"12","author":[{"given":"Aicha Yasmine","family":"Bellatreche","sequence":"first","affiliation":[{"name":"Ecole Nationale Sup\u00e9rieure V\u00e9t\u00e9rinaire, Rue Issad Abbes, Oued Smar, El Harrach, Algiers 16000, Algeria"},{"name":"Anses, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Laboratoire de Sant\u00e9 Animale, BIPAR, F-94700 Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riad","family":"Bouzid","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 Chadli Bendjedid El Tarf, BP 76, El Tarf 36000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandine","family":"Blaizot","sequence":"additional","affiliation":[{"name":"Anses, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Laboratoire de Sant\u00e9 Animale, BIPAR, F-94700 Maisons-Alfort, France"},{"name":"National Reference Laboratory for Animal Toxoplasmosis, Laboratoire de Sant\u00e9 Animale, ANSES, F-94700 Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Aubert","sequence":"additional","affiliation":[{"name":"EA 7510, UFR M\u00e9decine, University of Reims Champagne Ardenne, F-51095 Reims, France"},{"name":"Laboratory of Parasitology, National Reference Centre on Toxoplasmosis, Centre Hospitalier Universitaire de Reims, F-51095 Reims, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radu","family":"Blaga","sequence":"additional","affiliation":[{"name":"Anses, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Laboratoire de Sant\u00e9 Animale, BIPAR, F-94700 Maisons-Alfort, France"},{"name":"National Reference Laboratory for Animal Toxoplasmosis, Laboratoire de Sant\u00e9 Animale, ANSES, F-94700 Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khatima","family":"Ait-Oudhia","sequence":"additional","affiliation":[{"name":"Ecole Nationale Sup\u00e9rieure V\u00e9t\u00e9rinaire, Rue Issad Abbes, Oued Smar, El Harrach, Algiers 16000, Algeria"},{"name":"Laboratory of Biotechnology in Animal Reproduction, Universit\u00e9 de Blida, Blida 09000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3252-9155","authenticated-orcid":false,"given":"Delphine","family":"Le Roux","sequence":"additional","affiliation":[{"name":"Anses, INRAE, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Laboratoire de Sant\u00e9 Animale, BIPAR, F-94700 Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Darde","year":"2012","journal-title":"Clin. 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