{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:25:26Z","timestamp":1762071926377,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to evaluate the effects of GnRH administrated at day 35 after artificial insemination (AI) on the reproductive performance of buffalo cows. In ten buffalo farms in the period January\u2013February, 481 buffalo cows were subjected to estrus synchronization protocol and fixed-time artificial insemination (Ovsynch\u2013TAI program). Radioimmunoassays (RIA) for pregnancy-associated glycoproteins (PAGs) were used to detect pregnancy at day 28 after AI (cut-off value: \u22651 ng\/mL). Among pregnant subjects, those with PAG values between 1 and 2.5 ng\/mL were considered at risk of embryonic mortality (EM) and were assigned into two groups: treated (T; n = 57) control (C; n = 57). Treated buffaloes received 0.01 mg of buserelin acetate intramuscularly on day 35 after AI, whereas control buffaloes received no treatment. The pregnancy diagnosis was confirmed at day 60 through PAGs level and rectal palpation. The treatment with GnRH had a significant effect (p &lt; 0.001) in reducing EM. Between days 28 and 60 after AI, the animals that experienced EM were only 2\/57 in the T group, while were 13\/57 in the C group. Moreover, GnRH treatment produced a significant increase (p &lt; 0.001) in the PAG concentration between day 28 and day 60. Administration of GnRH at day 35 after AI in animals considered at risk of low embryo survival based on PAG levels allowed a reduction in pregnancy losses and improved the pregnancy rate during low-breeding season in buffalo.<\/jats:p>","DOI":"10.3390\/ani12202822","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T21:18:02Z","timestamp":1666127882000},"page":"2822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study"],"prefix":"10.3390","volume":"12","author":[{"given":"Corrado","family":"Pacelli","sequence":"first","affiliation":[{"name":"School of Agricultural, Forest, Food, and Environmental Sciences, University of Basilicata, 85100 Potenza, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittoria Lucia","family":"Barile","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA)\u2014Research Centre for Animal Production and Aquaculture, 00015 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1627-8926","authenticated-orcid":false,"given":"Emilio","family":"Sabia","sequence":"additional","affiliation":[{"name":"School of Agricultural, Forest, Food, and Environmental Sciences, University of Basilicata, 85100 Potenza, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Beatrice","family":"Casano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9824-0579","authenticated-orcid":false,"given":"Ada","family":"Braghieri","sequence":"additional","affiliation":[{"name":"School of Agricultural, Forest, Food, and Environmental Sciences, University of Basilicata, 85100 Potenza, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Martina","sequence":"additional","affiliation":[{"name":"School of Agricultural, Forest, Food, and Environmental Sciences, University of Basilicata, 85100 Potenza, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0828-4610","authenticated-orcid":false,"given":"Olimpia","family":"Barbato","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E128","DOI":"10.3168\/jds.S0022-0302(01)70207-X","article-title":"Reproductive management of dairy cows in high milk-producing herds","volume":"84","author":"Stevenson","year":"2001","journal-title":"J. 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