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Intestinal regionalization is necessary for normal physiological function, but it also means the research results obtained at specific sites may not be applicable to other intestinal segments. In order to comprehensively describe the functional changes in the intestine, different intestinal segments and their contents (duodenum, jejunum, ileum, cecum, colon, and rectum) of guinea pigs were collected for RNA seq and 16S rRNA seq, respectively. The results showed differential genes of each intestinal segment mainly involve mucosa, digestion, absorption, and immunity. The gene sets related to fat, bill salts, vitamins, aggregates, amino acids, and water absorption were highly expressed in the small intestine, and the gene sets related to metal ions, nucleotides, and SCFAs were highly expressed in the large intestine. In terms of immunity, the CD8+ T, Th1, eosinophils, pDCs, and natural killer (NK) T cells in the small intestine showed higher scores than those in the large intestine, while the pattern-recognition receptor signaling pathway-related genes are highly expressed in the large intestine. In terms of microbial composition, Proteobacteria and Actinobacteria are abundant in the small intestine, while Firmicutes and Spirochaete are abundant in large intestine. The correlation analysis showed a high correlation between intestinal microorganisms and gene modules related to digestion and absorption. In addition, cross-species analysis showed the SCFA metabolism gene expression trends in human and rodent intestine were different. In conclusion, we analyzed the changes in substance transport, immune and microbial composition between different intestinal segments of guinea pigs, and explored the relationship between intestinal transcriptome and microorganisms, our research will provides a reference for subsequent intestinal-related research.<\/jats:p>","DOI":"10.3390\/ani12212925","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T12:29:45Z","timestamp":1666700985000},"page":"2925","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Analysis of Transcriptomes and Microorganisms Reveals Differences between the Intestinal Segments of Guinea Pigs"],"prefix":"10.3390","volume":"12","author":[{"given":"Chuang","family":"Tang","sequence":"first","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jideng","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Chongqing Academy of Animal Sciences, Chongqing 402460, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanli","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinjiao","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yali","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoming","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianzi","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9974-7910","authenticated-orcid":false,"given":"Silu","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuewei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8681-7684","authenticated-orcid":false,"given":"Mingzhou","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1038\/s41385-021-00420-8","article-title":"Intestinal immune compartmentalization: Implications of tissue specific determinants in health and disease","volume":"14","author":"Brown","year":"2021","journal-title":"Mucosal Immunol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev.cellbio.042308.113344","article-title":"Vertebrate Endoderm Development and Organ Formation","volume":"25","author":"Zorn","year":"2009","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/nri3738","article-title":"Regional specialization within the intestinal immune system","volume":"14","author":"Mowat","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1146\/annurev.cellbio.20.010403.092805","article-title":"Signaling pathways in intestinal development and cancer","volume":"20","author":"Sancho","year":"2004","journal-title":"Annu Rev Cell Dev Biol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20191130","DOI":"10.1084\/jem.20191130","article-title":"Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine","volume":"217","author":"Wang","year":"2019","journal-title":"J. Exp. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/sj.embor.7400731","article-title":"The gut flora as a forgotten organ","volume":"7","author":"Shanahan","year":"2006","journal-title":"Embo Rep."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Diether, N., and Willing, B. (2019). Microbial Fermentation of Dietary Protein: An Important Factor in Diet\u2013Microbe\u2013Host Interaction. Microorganisms, 7.","DOI":"10.3390\/microorganisms7010019"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/s41385-021-00389-4","article-title":"Human gut-associated lymphoid tissues (GALT); diversity, structure, and function","volume":"14","author":"Fenton","year":"2021","journal-title":"Mucosal Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"G341","DOI":"10.1152\/ajpgi.00046.2013","article-title":"Studies of mucus in mouse stomach, small intestine, and colon. I. Gastrointestinal mucus layers have different properties depending on location as well as over the Peyer\u2019s patches","volume":"305","author":"Ermund","year":"2013","journal-title":"AJP Gastrointest. Liver Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nri3608","article-title":"Intestinal epithelial cells: Regulators of barrier function and immune homeostasis","volume":"14","author":"Peterson","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/s41579-018-0036-x","article-title":"Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier","volume":"16","author":"Martens","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"584288","DOI":"10.3389\/fimmu.2020.584288","article-title":"Host factors of favorable intestinal microbial colonization","volume":"11","author":"Pirr","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.chom.2019.08.011","article-title":"Regional Diversity of the Gastrointestinal Microbiome","volume":"26","author":"Leone","year":"2019","journal-title":"Cell. Host. Microbe."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1124\/dmd.117.077313","article-title":"RNA-Seq Profiling of Intestinal Expression of Xenobiotic Processing Genes in Germ-Free Mice","volume":"45","author":"Fu","year":"2017","journal-title":"Drug Metab. Dispos. Biol. Fate Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1136\/gutjnl-2011-301104","article-title":"Analysis of gut microbial regulation of host gene expression along the length of the gut and regulation of gut microbial ecology through MyD88","volume":"61","author":"Larsson","year":"2012","journal-title":"Gut"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/ame2.12022","article-title":"The role of the gut microbiota on animal model reproducibility","volume":"1","author":"Turner","year":"2018","journal-title":"Anim. Model. Exp. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Hildebrand, F., Ebersbach, T., Nielsen, H., Li, X., Sonne, S., Bertalan, M., Dimitrov, P., Madsen, L., Qin, J., and Wang, J. (2012). A comparative analysis of the intestinal metagenomes present in guinea pigs (Cavia porcellus) and humans (Homo sapiens). Bmc Genom., 13.","DOI":"10.1186\/1471-2164-13-514"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Elfers, K., Armbrecht, Y., and Mazzuoli-Weber, G. (2021). Good to Know: Baseline Data on Feed Intake, Fecal Pellet Output and Intestinal Transit Time in Guinea Pig as a Frequently Used Model in Gastrointestinal Research. Animals, 11.","DOI":"10.3390\/ani11061593"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ejps.2010.09.019","article-title":"Assessment of gastrointestinal pH, fluid and lymphoid tissue in the guinea pig, rabbit and pig, and implications for their use in drug development","volume":"42","author":"Merchant","year":"2011","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"845","DOI":"10.3389\/fgene.2020.00845","article-title":"Comprehensive Analysis of mRNA and lncRNA Transcriptomes Reveals the Differentially Hypoxic Response of Preadipocytes during Adipogenesis","volume":"11","author":"Zhang","year":"2020","journal-title":"Front Genet."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Feng, S., Ma, J., Long, K., Zhang, J., Qiu, W., Li, Y., Jin, L., Wang, X., Jiang, A., and Liu, L. (2020). Comparative microRNA transcriptomes in domestic goats reveal acclimatization to high altitude. Front. Genet., 809.","DOI":"10.3389\/fgene.2020.00809"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1126\/science.1230612","article-title":"The evolutionary landscape of alternative splicing in vertebrate species","volume":"338","author":"Irimia","year":"2012","journal-title":"Science"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1093\/bioinformatics\/btp536","article-title":"QuickGO: A web-based tool for Gene Ontology searching","volume":"25","author":"Binns","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-017-1349-1","article-title":"xCell: Digitally portraying the tissue cellular heterogeneity landscape","volume":"18","author":"Aran","year":"2017","journal-title":"Genome Biol."},{"key":"ref_25","first-page":"1","article-title":"An RNA-Seq atlas of gene expression in mouse and rat normal tissues","volume":"4","author":"Leparc","year":"2017","journal-title":"Sci. Data"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"508","DOI":"10.3389\/fgene.2018.00508","article-title":"Weighted gene correlation network analysis (WGCNA) reveals novel transcription factors associated with bisphenol A dose-response","volume":"9","author":"Maertens","year":"2018","journal-title":"Front. Gene.t"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"14S","DOI":"10.1177\/0148607113520005","article-title":"Pathophysiology of short bowel syndrome: Considerations of resected and residual anatomy","volume":"38","author":"Tappenden","year":"2014","journal-title":"J. Parenter. Enter. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.smim.2015.03.009","article-title":"PRR-signaling pathways\u2013Learning from microbial tactics","volume":"27","author":"Sellge","year":"2015","journal-title":"Semin. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: An R package for weighted correlation network analysis. BMC Bioinform., 9.","DOI":"10.1186\/1471-2105-9-559"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Borrello, K., Lim, U., Park, S., Monroe, K.R., Maskarinec, G., Boushey, C.J., Wilkens, L.R., Randolph, T.W., Le Marchand, L., and Hullar, M.A. (2022). Dietary Intake Mediates Ethnic Differences in Gut Microbial Composition. Nutrients, 14.","DOI":"10.3390\/nu14030660"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, B., Kong, Q., Li, X., Zhao, J., Zhang, H., Chen, W., and Wang, G. (2020). A high-fat diet increases gut microbiota biodiversity and energy expenditure due to nutrient difference. Nutrients, 12.","DOI":"10.3390\/nu12103197"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1093\/ajcn\/nqz139","article-title":"Dietary quality and the colonic mucosa\u2013associated gut microbiome in humans","volume":"110","author":"Liu","year":"2019","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.3389\/fmicb.2017.01818","article-title":"Illumina sequencing approach to characterize thiamine metabolism related bacteria and the impacts of thiamine supplementation on ruminal microbiota in dairy cows fed high-grain diets","volume":"8","author":"Pan","year":"2017","journal-title":"Front Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1096\/fj.201901943RR","article-title":"Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition","volume":"34","author":"Liu","year":"2020","journal-title":"Faseb. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6873","DOI":"10.1039\/C9FO00296K","article-title":"Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota","volume":"10","author":"Guo","year":"2019","journal-title":"Food Funct."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1007\/s11095-006-9041-2","article-title":"Why is it challenging to predict intestinal drug absorption and oral bioavailability in human using rat model","volume":"23","author":"Cao","year":"2006","journal-title":"Pharm Res-Dordr."},{"key":"ref_37","first-page":"e10","article-title":"Regulation of intestinal epithelial barrier function by long noncoding RNA uc","volume":"38","author":"Wang","year":"2018","journal-title":"173 through interaction with microRNA 29b. Mol. Cell. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1423-0127-20-87","article-title":"LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn\u2019s disease","volume":"20","author":"Qiao","year":"2013","journal-title":"J. Biomed. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chen, L., Zhang, Y., Pan, X., Liu, M., Wang, S., Huang, T., and Cai, Y. (2018). Tissue expression difference between mRNAs and lncRNAs. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113416"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1002\/jcb.22090","article-title":"Expression of GLUT8 in mouse intestine: Identification of alternative spliced variants","volume":"106","author":"Romero","year":"2009","journal-title":"J. Cell. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"681","DOI":"10.3109\/00365521.2014.898326","article-title":"Surface area of the digestive tract\u2013revisited","volume":"49","author":"Helander","year":"2014","journal-title":"Scand. J. Gastroentero."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1365-2982.2012.01921.x","article-title":"Intestinal barrier function in health and gastrointestinal disease","volume":"24","author":"Camilleri","year":"2012","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-7075-7-19","article-title":"Dietary fat and bile juice, but not obesity, are responsible for the increase in small intestinal permeability induced through the suppression of tight junction protein expression in LETO and OLETF rats","volume":"7","author":"Suzuki","year":"2010","journal-title":"Nutr. Metab."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.addr.2006.01.003","article-title":"Tight junction modulation and its relationship to drug delivery","volume":"58","author":"Salama","year":"2006","journal-title":"Adv. Drug Deliver. Rev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5056","DOI":"10.1074\/jbc.M808128200","article-title":"Slc2a5 (Glut5) is essential for the absorption of fructose in the intestine and generation of fructose-induced hypertension","volume":"284","author":"Barone","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.mam.2012.11.002","article-title":"Glucose transport families SLC5 and SLC50","volume":"34","author":"Wright","year":"2013","journal-title":"Mol. Aspects. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1146\/annurev.nutr.28.061807.155518","article-title":"Sugar absorption in the intestine: The role of GLUT2","volume":"28","author":"Kellett","year":"2008","journal-title":"Annu. Rev. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.bbaexp.2005.06.012","article-title":"Functional characterization of the promoter of the human glucose transporter 10 gene","volume":"1730","author":"Segade","year":"2005","journal-title":"Biochim. Et Biophys. Acta. Gene. Struct. Expr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.atherosclerosissup.2010.03.002","article-title":"Update on genetics of postprandial lipemia","volume":"11","year":"2010","journal-title":"Atheroscler. Supp."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.biochi.2013.08.026","article-title":"Perilipins: Lipid droplet coat proteins adapted for tissue-specific energy storage and utilization, and lipid cytoprotection","volume":"96","author":"Sztalryd","year":"2014","journal-title":"Biochimie"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/s41580-019-0190-7","article-title":"Mechanisms and regulation of cholesterol homeostasis","volume":"21","author":"Luo","year":"2020","journal-title":"Nat. Rev. Mol. Cell. Bio."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"158658","DOI":"10.1016\/j.bbalip.2020.158658","article-title":"Role of lipids in pathophysiology, diagnosis and therapy of hepatocellular carcinoma","volume":"1865","author":"Buechler","year":"2020","journal-title":"Biochim. Biophys. Acta. Mol. Cell. Biol. Lipids"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ajps.2020.02.005","article-title":"Glutamine transporters as pharmacological targets: From function to drug design","volume":"15","author":"Scalise","year":"2020","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Monn\u00e9, M., Vozza, A., Lasorsa, F.M., Porcelli, V., and Palmieri, F. (2019). Mitochondrial carriers for aspartate, glutamate and other amino acids: A review. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184456"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1017\/S1751731118001404","article-title":"Rabbit SLC15A1, SLC7A1 and SLC1A1 genes are affected by site of digestion, stage of development and dietary protein content","volume":"13","author":"Liu","year":"2019","journal-title":"Animal"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Feichtinger, R.G., and Lang, R. (2019). Targeting L-lactate metabolism to overcome resistance to immune therapy of melanoma and other tumor entities. J. Oncol., 1\u201312.","DOI":"10.1155\/2019\/2084195"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.tibs.2018.05.003","article-title":"Amino acid transporters revisited: New views in health and disease","volume":"43","author":"Kandasamy","year":"2018","journal-title":"Trends Biochem. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.mam.2012.07.004","article-title":"The solute carrier family 10 (SLC10): Beyond bile acid transport","volume":"34","author":"Polli","year":"2013","journal-title":"Mol. Aspects Med."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Praslickova, D., Torchia, E.C., Sugiyama, M.G., Magrane, E.J., Zwicker, B.L., Kolodzieyski, L., and Agellon, L.B. (2012). The ileal lipid binding protein is required for efficient absorption and transport of bile acids in the distal portion of the murine small intestine. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050810"},{"key":"ref_60","first-page":"1894","article-title":"A frequent 1085delC\/insGAAG mutation in the RDH5 gene in Japanese patients with fundus albipunctatus","volume":"41","author":"Wada","year":"2000","journal-title":"Invest. Ophth. Vis. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"10435","DOI":"10.1073\/pnas.0701382104","article-title":"Synthetic ecosystems based on airborne inter-and intrakingdom communication","volume":"104","author":"Weber","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3627","DOI":"10.1093\/hmg\/ddy267","article-title":"Mice lacking the transcobalamin-vitamin B12 receptor, CD320, suffer from anemia and reproductive deficits when fed vitamin B12-deficient diet","volume":"27","author":"Bernard","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Zhu, C., Chen, Z., and Jiang, Z. (2016). Expression, distribution and role of aquaporin water channels in human and animal stomach and intestines. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17091399"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1078\/0171-9335-00210","article-title":"Expression and immunolocalization of the aquaporin-8 water channel in rat gastrointestinal tract","volume":"80","author":"Calamita","year":"2001","journal-title":"Eur. J. Cell. Biol."},{"key":"ref_65","first-page":"8047","article-title":"Hypermethylation of SCNN1A gene-body increases the risk of essential hypertension","volume":"9","author":"Mao","year":"2016","journal-title":"Int. J. Clin. Exp. Pathol"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5732","DOI":"10.3748\/wjg.v25.i38.5732","article-title":"Role of ion channels in gastrointestinal cancer","volume":"25","author":"Anderson","year":"2019","journal-title":"World. J. Gastroentero."},{"key":"ref_67","first-page":"1","article-title":"Identification of uterine ion transporters for mineralisation precursors of the avian eggshell","volume":"12","author":"Brionne","year":"2012","journal-title":"BMC Physiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40246-019-0233-3","article-title":"SLC39A8 gene encoding a metal ion transporter: Discovery and bench to bedside","volume":"13","author":"Nebert","year":"2019","journal-title":"Hum. Genom."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1080\/17460441.2017.1378176","article-title":"Absorption sites of orally administered drugs in the small intestine","volume":"12","author":"Murakami","year":"2017","journal-title":"Expert Opin. Drug Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"5514","DOI":"10.3168\/jds.2019-17309","article-title":"Propionate enhances the expression of key genes involved in the gluconeogenic pathway in bovine intestinal epithelial cells","volume":"103","author":"Zhan","year":"2020","journal-title":"J. Dairy. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.cell.2013.12.016","article-title":"Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits","volume":"156","author":"Goncalves","year":"2014","journal-title":"Cell"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"S536","DOI":"10.21037\/tcr.2016.08.24","article-title":"Colonic crypts are natural gatekeepers of microbial metabolites to protect stem cells","volume":"5","author":"Vemula","year":"2016","journal-title":"Transl. Cancer Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1046\/j.1344-3941.2003.00124.x","article-title":"Digestive strategies of small hindgut fermenters","volume":"74","author":"Sakaguchi","year":"2003","journal-title":"Anim. Sci. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1042\/bj3600265","article-title":"Dietary and developmental regulation of intestinal sugar transport","volume":"360","author":"Ferraris","year":"2001","journal-title":"Biochem. J."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/jcb.10360","article-title":"Intestinal calcium absorption: Molecular vitamin D mediated mechanisms","volume":"88","author":"Bouillon","year":"2003","journal-title":"J. Cell. Biochem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3389\/fimmu.2012.00328","article-title":"NOD1 and NOD2 signaling in infection and inflammation","volume":"3","author":"Moreira","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Wang, Y., Devkota, S., Musch, M.W., Jabri, B., Nagler, C., Antonopoulos, D.A., Chervonsky, A., and Chang, E.B. (2010). Regional mucosa-associated microbiota determine physiological expression of TLR2 and TLR4 in murine colon. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0013607"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nm.2087","article-title":"Recognition of peptidoglycan from the microbiota by Nod1 enhances systemic innate immunity","volume":"16","author":"Clarke","year":"2010","journal-title":"Nat. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1126\/science.1209791","article-title":"The antibacterial lectin RegIII\u03b3 promotes the spatial segregation of microbiota and host in the intestine","volume":"334","author":"Vaishnava","year":"2011","journal-title":"Science"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1053\/j.gastro.2015.10.046","article-title":"Leukocyte trafficking to the small intestine and colon","volume":"150","author":"Habtezion","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"6347","DOI":"10.1073\/pnas.0609180104","article-title":"CCR9 is a homing receptor for plasmacytoid dendritic cells to the small intestine","volume":"104","author":"Wendland","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial signature of dysbiosis in gut microbiota","volume":"33","author":"Shin","year":"2015","journal-title":"Trends Biotechnol."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Stojanov, S., Berlec, A., and \u0160trukelj, B. (2020). The influence of probiotics on the firmicutes\/bacteroidetes ratio in the treatment of obesity and inflammatory bowel disease. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111715"},{"key":"ref_84","unstructured":"Tsinganou, E., and Gebbers, J. (2010). Human intestinal spirochetosis\u2013A review. GMS Ger. Med. Sci., 8."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/nature12687","article-title":"Histone deacetylase 3 coordinates commensal-bacteria-dependent intestinal homeostasis","volume":"504","author":"Alenghat","year":"2013","journal-title":"Nature"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3177\/jnsv.66.60","article-title":"Significant species differences in intestinal phosphate absorption between dogs, rats, and monkeys","volume":"66","author":"Ichida","year":"2020","journal-title":"J. Nutr. Sci. Vitaminol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/21\/2925\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:02:26Z","timestamp":1760144546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/21\/2925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,25]]},"references-count":86,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ani12212925"],"URL":"https:\/\/doi.org\/10.3390\/ani12212925","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2022,10,25]]}}}