{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:35:14Z","timestamp":1790951714666,"version":"4.1.0"},"reference-count":48,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ELANCO ANIMAL HEALT","award":["35295"],"award-info":[{"award-number":["35295"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The presence of \u03b2-mannans in feed can produce a futile and chronic immune stimulation in fattening pigs. In this trial, a 1-4-endo-D-\u03b2-mannanase was added to the feed (HC) during growth and fattening (0.03% of Hemicell HT) and physical performance and pathological data were recorded, and intestinal integrity and immune activation were studied by molecular biomarkers, compared to a control group (CON). The treatment diet was reduced in energy content by 40 Kcal\/kg NE. From each group, 113 and 112 animals housed in 8 pens were individually identified and weighed three times: at 7th, 63rd and 116th days in feed. The FCR was calculated for groups of two pens and ADG individually. There was no difference in ADG (CON = 0.836, HC = 0.818) nor in FCR between groups (p = 0.486). During growth, there was a higher frequency of normal feces in HC and there were also no differences in the frequency of gastric lesions. A significant increase in Claudin, Occludin, IFN-\u03b3 and IL8 was observed in the CON in feces and a significant decrease in IL-6 in HC. In tissues, there were differences for IL-12p40, TNF-alpha in jejunum (increased CON) and TGF-\u03b2 in ileum and jejunum, (decreased HC). The economic performance was EUR 4.7 better in the treated group. In conclusion, the addition of 1-4-endo-D-\u03b2--mannanase to the feed with a 1.6% reduction in net energy compared to the control, allowed the animals to perform as well as the animals on the higher energy diet, with lower prevalence of diarrhea.<\/jats:p>","DOI":"10.3390\/ani12213012","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T03:11:21Z","timestamp":1667445081000},"page":"3012","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effect of \u03b2-Mannanase Addition during Whole Pigs Fattening on Production Yields and Intestinal Health"],"prefix":"10.3390","volume":"12","author":[{"given":"Pedro","family":"S\u00e1nchez-Uribe","sequence":"first","affiliation":[{"name":"ELANCO Animal Health, 20108 Alcobendas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8861-6322","authenticated-orcid":false,"given":"Eva","family":"Romera-Recio","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn (CSIC), 18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina G.","family":"Cabrera-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa V.","family":"Hern\u00e1ndez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"Lamrani","sequence":"additional","affiliation":[{"name":"ELANCO Animal Health, 20108 Alcobendas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"Gonz\u00e1lez-Guijarro","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"de Pascual-Monreal","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Mendon\u00e7a-Pascoal","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s, Goi\u00e1nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7497-6709","authenticated-orcid":false,"given":"Laura","family":"Mart\u00ednez-Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto Murciano de Investigaci\u00f3n en Biomedicina (IMIB), 30100 Murcia, Spain"},{"name":"UDICA, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5580-1612","authenticated-orcid":false,"given":"Guillermo","family":"Ramis","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain"},{"name":"Instituto Murciano de Investigaci\u00f3n en Biomedicina (IMIB), 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"ref_1","unstructured":"Committee for Medicinal Products for Veterinary Use (CVMP) (2016). 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