{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T17:49:42Z","timestamp":1767203382245,"version":"build-2238731810"},"reference-count":40,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102695"],"award-info":[{"award-number":["32102695"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011136","name":"State Key Laboratory of Veterinary Etiological Biology","doi-asserted-by":"publisher","award":["SKLVEB2021KFKT008"],"award-info":[{"award-number":["SKLVEB2021KFKT008"]}],"id":[{"id":"10.13039\/501100011136","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["SWU-KT22014"],"award-info":[{"award-number":["SWU-KT22014"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Animals"],"abstract":"<jats:p>As there are few studies of Babesia spp. infection in cats in China, or anywhere in the world, the aim of this study was to explore the epidemic features of babesiosis in pet cats in China. In total, 429 blood samples were randomly collected in four different geographical regions. The 18S rRNA gene fragment of Babesia spp. was amplified by nest polymerase chain reaction (PCR), and haplotype and phylogenetic analysis of Babesia were performed to analyze the relationship of this protozoa. The total positive rate of infection was 2.8%. BLAST analysis indicated that Babesia gibsoni was detected in 12 cats. Among these, 4.3%, 3.1%, 0.8% and 2.0% were from Chongqing, Fujian, Hubei and Shandong, respectively. Haplotype and phylogenetic analysis showed that there were nine haplotypes and no obvious genetic variation among B. gibsoni populations. These findings will be helpful for understanding the epidemiology of Babesia spp. in China, and provide a foundation for developing effective preventative strategies.<\/jats:p>","DOI":"10.3390\/ani12223066","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T11:31:51Z","timestamp":1667907111000},"page":"3066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Detection of Babesia gibsoni in Cats in China"],"prefix":"10.3390","volume":"12","author":[{"given":"Fangyuan","family":"Yin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daoe","family":"Mu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuojia","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiting","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2076-3764","authenticated-orcid":false,"given":"Jinming","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiquan","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Facai","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1177\/0300985809354465","article-title":"Global climate change and implications for disease emergence","volume":"47","author":"Slenning","year":"2010","journal-title":"Vet. Pathol."},{"key":"ref_2","first-page":"56","article-title":"Present Situation and Development Trend of Pet Industry in China","volume":"8","author":"Chen","year":"2019","journal-title":"China Clean. Ind."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e0026620","DOI":"10.1128\/CMR.00266-20","article-title":"Canine and feline parasitology: Analogies, differences, and relevance for human health","volume":"34","author":"Morelli","year":"2021","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.vetpar.2017.12.021","article-title":"Arthropod-borne pathogens of dogs and cats: From pathways and times of transmission to disease control","volume":"251","author":"Otranto","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1186\/s13071-016-1596-0","article-title":"A review of canine babesiosis: The European perspective","volume":"9","author":"Sainz","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Almazan, C., Scimeca, R.C., Reichard, M.V., and Mosqueda, J. (2022). Babesiosis and Theileriosis in North America. Pathogens, 11.","DOI":"10.3390\/pathogens11020168"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1556\/avet.54.2006.3.7","article-title":"Clinical manifestations of canine babesiosis in Hungary (63 cases)","volume":"54","author":"Mathe","year":"2006","journal-title":"Acta. Vet. Hung."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1476-4431.2009.00493.x","article-title":"Feline babesiosis","volume":"20","author":"Ayoob","year":"2010","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_9","first-page":"119","article-title":"Canine babesiosis: A perspective on clinical complications, biomarkers, and treatment","volume":"6","author":"Koster","year":"2015","journal-title":"Vet. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/j.cvsm.2010.08.001","article-title":"Canine babesiosis","volume":"40","author":"Irwin","year":"2010","journal-title":"Vet. Clin. Small Anim. Pract."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.pt.2011.11.003","article-title":"Canine babesiosis in Europe: How many diseases?","volume":"28","author":"Matijatko","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.vetpar.2013.03.015","article-title":"Autochthonous canine babesiosis caused by Babesia canis canis in Latvia","volume":"196","author":"Berzina","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/s13071-015-0830-5","article-title":"Reclassification of Theileria annae as Babesia vulpes sp. nov","volume":"8","author":"Baneth","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1186\/s13071-015-1120-y","article-title":"Molecular detection of vector-borne agents in dogs from ten provinces of China","volume":"8","author":"Xu","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.3389\/fmicb.2017.01577","article-title":"First molecular detection of Babesia gibsoni in dogs from Wuhan, China","volume":"8","author":"He","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3389\/fmicb.2017.01029","article-title":"First molecular detection of piroplasm infection in pet dogs from Gansu, China","volume":"8","author":"Niu","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1292\/jvms.16-0484","article-title":"First molecular detection of tick-borne pathogens in dogs from Jiangxi, China","volume":"79","author":"Zheng","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.parint.2019.03.011","article-title":"Molecular detection and genetic diversity of Babesia canis canis in pet dogs in Henan Province, China","volume":"71","author":"Wang","year":"2019","journal-title":"Parasitol. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3389\/fvets.2020.00134","article-title":"Babesia species of domestic cats: Molecular characterization has opened pandora\u2019s box","volume":"7","author":"Penzhorn","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1177\/1098612X13489230","article-title":"Babesiosis in cats: ABCD guidelines on prevention and management","volume":"15","author":"Hartmann","year":"2013","journal-title":"J. Feline Med. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kelly, P.J., Koster, L., Li, J., Zhang, J., Huang, K., Branford, G.C., Marchi, S., Vandenplas, M., and Wang, C. (2017). Survey of vector-borne agents in feral cats and first report of Babesia gibsoni in cats on St Kitts, West Indies. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1230-1"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhang, X.L., Li, X.W., Li, W.J., Huang, H.L., Huang, S.J., and Shao, J.W. (2019). Molecular evidence of Babesia in pet cats in mainland China. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2214-0"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1128\/JCM.01300-12","article-title":"Detection of Babesia hongkongensis sp. nov. in a free-roaming felis catus cat in Hong Kong","volume":"50","author":"Wong","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, J., Wang, X., Sun, H., Lv, Z., Li, Y., Luo, J., Guan, G., and Yin, H. (2020). Molecular evidence of piroplasm infection in companion animals in Hunan Province, China. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02500-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.parint.2014.06.002","article-title":"Molecular evidence for piroplasms in wild Reeves\u2019 muntjac (Muntiacus reevesi) in China","volume":"63","author":"Yang","year":"2014","journal-title":"Parasitol. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","first-page":"14","article-title":"GeneDoc: Analysis and visualization of genetic variation","volume":"4","author":"Nicholas","year":"1997","journal-title":"Embnew. News"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1046\/j.1365-294x.2000.01020.x","article-title":"TCS: A computer program to estimate gene genealogies","volume":"9","author":"Clement","year":"2000","journal-title":"Mol. Ecol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"PopART: Full-feature software for haplotype network construction","volume":"6","author":"Leigh","year":"2015","journal-title":"Methods Ecol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetpar.2004.07.028","article-title":"First molecular detection of Babesia vogeli in dogs from Brazil","volume":"127","author":"Passos","year":"2005","journal-title":"Vet. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.vetpar.2010.06.025","article-title":"Detection of Babesia vogeli in stray cats of metropolitan Bangkok, Thailand","volume":"173","author":"Simking","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/vcp.12451","article-title":"Acute phase proteins response in cats naturally infected with Hepatozoon felis and Babesia vogeli","volume":"46","author":"Vilhena","year":"2017","journal-title":"Vet. Clin. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.3201\/eid2606.191832","article-title":"Zoonotic vectorborne pathogens and ectoparasites of dogs and cats in eastern and southeast Asia","volume":"26","author":"Colella","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.vetpar.2015.10.002","article-title":"Seroprevalence survey of Babesia gibsoni infection and tick species in dogs in East China","volume":"214","author":"Cao","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1186\/s13071-020-04232-w","article-title":"Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China","volume":"13","author":"Guo","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"101729","DOI":"10.1016\/j.ttbdis.2021.101729","article-title":"PCR detection and genetic characterization of piroplasms from dogs in Myanmar, and a possible role of dogs as reservoirs for Theileria parasites infecting cattle, water buffaloes, and goats","volume":"12","author":"Bawm","year":"2021","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s10493-010-9335-2","article-title":"Ticks (acari: Ixodoidea: Argasidae, ixodidae) of China","volume":"51","author":"Chen","year":"2010","journal-title":"Exp. Appl. Acarol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e21544","DOI":"10.1002\/arch.21544","article-title":"Ticks (Acari: Ixodoidea) in China: Geographical distribution, host diversity, and specificity","volume":"102","author":"Zhang","year":"2019","journal-title":"Arch. Insect. Biochem. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"959","DOI":"10.2460\/javma.2003.222.959","article-title":"Transfusion-associated Babesia gibsoni infection in a dog","volume":"222","author":"Stegeman","year":"2003","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1751-0813.2007.00220.x","article-title":"Blood, Bull Terriers and Babesiosis: Further evidence for direct transmission of Babesia gibsoni in dogs","volume":"85","author":"Jefferies","year":"2007","journal-title":"Aust. Vet. J."}],"updated-by":[{"DOI":"10.3390\/ani13010138","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000}}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3066\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T22:38:10Z","timestamp":1754260690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,8]]},"references-count":40,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ani12223066"],"URL":"https:\/\/doi.org\/10.3390\/ani12223066","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,8]]}}}