{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:36:11Z","timestamp":1787841371373,"version":"build-2784847793"},"reference-count":36,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003046","name":"Brazilian Agricultural Research Corporation","doi-asserted-by":"publisher","award":["03.16.05.004"],"award-info":[{"award-number":["03.16.05.004"]}],"id":[{"id":"10.13039\/501100003046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Nicarbazin (NCZ) is a worldwide used anticoccidial in poultry farming to avoid coccidiosis disease when chickens are reared on conventional poultry litter. If proper dosage and withdrawal time are not followed, the component dinitrocarbanilide (DNC) of NCZ may be present in chicken tissues, therefore posing a risk to consumers if the residues are above 200 \u00b5g\/kg. Litter reuse is a common and important practice in commercial chicken production. Literature is lacking about the influence of litter reuse on DNC deposition in chicken tissues and its impact on food safety. We aimed to evaluate DNC residues in breast and liver by LC-MS\/MS from broilers from an experiment with 10 consecutive flocks during 2 years. The experiment included three treatments containing NCZ in the diet (T1 = 125 mg\/kg, 1\u201321 d; T2 = 125 mg\/kg, 1\u201332 d; T3 = 40 mg\/kg, 1\u201332 d). DNC residues in chicken breast at 21 d in T1 ranged from 648.8\u2013926 \u00b5g\/kg, at 32 d in T2 and T3 varied, respectively, from 232\u2013667 \u00b5g\/kg and 52\u2013189 \u00b5g\/kg. Regarding liver, DNC residues at 21 days in T1 ranged from 11,754\u201315,281 \u00b5g\/kg, at 32 days in T2 and T3 varied, respectively, from 10,168\u201315,021 \u00b5g\/kg and 2899\u20134573 \u00b5g\/kg. When NCZ was withdrawn from feed, DNC residues dropped to &lt;LOQ at 42 d in all treatments. Therefore, the reuse of poultry litter does not compromise food safety regarding DNC residues in chicken tissues, as shown herein up to 10 flocks.<\/jats:p>","DOI":"10.3390\/ani12223107","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T19:18:29Z","timestamp":1668107909000},"page":"3107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Nicarbazin Residue in Tissues from Broilers Reared on Reused Litter Conditions"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8678-2415","authenticated-orcid":false,"given":"Vivian","family":"Feddern","sequence":"first","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson N.","family":"Scheuermann","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlei","family":"Coldebella","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3162-2630","authenticated-orcid":false,"given":"Vanessa","family":"Gressler","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gizelle C.","family":"Bedendo","sequence":"additional","affiliation":[{"name":"Embrapa Solos [Embrapa Soil], Setor de Transfer\u00eancia de Tecnologia, Jardim Bot\u00e2nico 1024, Rio de Janeiro 22460-000, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luizinho","family":"Caron","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio C.","family":"Pedroso","sequence":"additional","affiliation":[{"name":"Instituto Federal Catarinense, Conc\u00f3rdia 89703-720, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danniele M.","family":"Bacila","sequence":"additional","affiliation":[{"name":"Departamento de Engenharia Qu\u00edmica, Universidade Federal do Paran\u00e1, Curitiba 80060-000, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Anildo","family":"Cunha","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves [Embrapa Swine and Poultry], BR 153, km 110, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pt.2013.10.003","article-title":"Securing Poultry Production from the Ever-Present Eimeria Challenge","volume":"30","author":"Blake","year":"2014","journal-title":"Trends Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3382\/ps.2013-03634","article-title":"Milestones in Avian Coccidiosis Research: A Review","volume":"93","author":"Chapman","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1126\/science.122.3162.244.b","article-title":"Antiparasitic Activity of Substituted Carbanilide Complexes","volume":"122","author":"Cuckler","year":"1955","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.3382\/ps.0351355","article-title":"Biological Studies on Nicarbazin, a New Anticoccidial Agent","volume":"35","author":"Ott","year":"1956","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.taap.2010.12.014","article-title":"Risk Assessment of Coccidostatics during Feed Cross-Contamination: Animal and Human Health Aspects","volume":"270","author":"Dorne","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.meatsci.2014.01.004","article-title":"A Review of Coccidiostats and the Analysis of Their Residues in Meat and Other Food","volume":"97","author":"Clarke","year":"2014","journal-title":"Meat Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.foodres.2017.07.011","article-title":"Current Research, Regulation, Risk, Analytical Methods and Monitoring Results for Nicarbazin in Chicken Meat: A Perspective Review","volume":"99","author":"Bacila","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_8","unstructured":"(2021). Code of Federal Regulation 21 CFR \u00a7 558.364\u2014Narasin and Nicarbazin, Code of Federal Regulations (Annual Edition)."},{"key":"ref_9","unstructured":"EC Commission Regulation (EU) (2010). No 875\/2010 of 5 October 2010 Concerning the Authorisation for 10 Years of an Additive in Feedingstuffs. Off. J. Eur. Union, 263, 4\u20136."},{"key":"ref_10","unstructured":"FAO\/WHO (2018). Maximum Residue Limits (MRLs) and Risk Management Recommendations (RMRs) for Residues of Veterinary Drugs in Foods, FAO\/WHO. CX\/MRL 2-2018."},{"key":"ref_11","unstructured":"BRASIL Ministry of Agriculture, Livestock and Food Supply (2022, October 27). National Plan for the Control of Residues and Contaminants; 2020. Results of the National Plan for the Control of Residues and Contaminants\u2014PNCRC, Available online: https:\/\/www.gov.br\/agricultura\/pt-br\/centrais-de-conteudo\/assuntos\/inspecao\/produtos-animal\/plano-de-nacional-de-controle-de-residuos-e-contaminantes\/plano-de-nacional-de-controle-de-residuos-e-contaminantes#section-1."},{"key":"ref_12","first-page":"947","article-title":"Nicarbazina: Deplezione Della Componente Dnc Dal Tessuto Muscolare Ed Epatico Di Broilers","volume":"28","author":"Castelli","year":"1989","journal-title":"Ind. Aliment."},{"key":"ref_13","unstructured":"Kan, C.A., Keukens, H.J., and Boers, E. (1996, January 6\u20138). Nicarbazin Residues in Broiler Muscle, Liver and Litter\/Faeces: Early Exposure or Recirculation. Proceedings of the EuroResidue III Conference, Veldhoven, The Netherlands."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1093\/japr\/8.3.292","article-title":"Residues in Broiler Tissue and Litter","volume":"8","author":"Penz","year":"1999","journal-title":"Appl. Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1080\/0265203001001325","article-title":"Possible Causes of Nicarbazin Residues in Chicken Tissues","volume":"17","author":"Cannavan","year":"2000","journal-title":"Food Addit. Contam."},{"key":"ref_16","first-page":"761","article-title":"Depletion Study on Nicarbazin and Narasin in Tissues and Eggs of Hens Housed in Deep Litter","volume":"55","author":"Olejnik","year":"2011","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_17","unstructured":"CONCEA (2013). Diretriz Brasileira Para o Cuidado e a Utiliza\u00e7\u00e3o de Animais Para Fins Cient\u00edficos e Did\u00e1tico [Brazilian Guideline for the Care and Use of Animals for Scientific and Educational Purposes], CONCEA."},{"key":"ref_18","unstructured":"Rostagno, H.S. (2011). Tabelas Brasileiras Para Aves e Su\u00ednos: Composi\u00e7\u00e3o de Alimentos e Exig\u00eancias Nutricionais [Brazilian Tables for Poultry and Swine: Food Composition and Nutritional Requirements], Universidade Federal de Vi\u00e7osa (UFV). [3rd ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1017\/S0043933918000909","article-title":"Wood-Based Litter in Poultry Production: A Review","volume":"75","author":"Munir","year":"2019","journal-title":"World\u2019S Poult. Sci. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1080\/00032719.2015.1025277","article-title":"Determination of Nicarbazin in Animal Feed by High-Performance Liquid Chromatography with Interlaboratory Evaluation","volume":"48","author":"Protasiuk","year":"2015","journal-title":"Anal. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"630","DOI":"10.5740\/jaoacint.13-197","article-title":"Determination and Confirmation of Nicarbazin, Measured as 4,4-Dinitrocarbanilide (DNC), in Chicken Tissues by Liquid Chromatography with Tandem Mass Spectrometry: First Action 2013.07","volume":"97","author":"Coleman","year":"2014","journal-title":"J. AOAC Int."},{"key":"ref_22","unstructured":"(Sas Institute Inc System for Microsoft Windows, 2012). Sas Institute Inc System for Microsoft Windows, Release 9.4; (CD-ROM)."},{"key":"ref_23","unstructured":"Xavier, L.H. (2019). Univariate and Multivariate Models for Analysis of Repeated Measures and Verification of the Accuracy of the Univariate Model through Simulation. [Master\u2019s Dissertation, University of S\u00e3o Paulo]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1002\/jeq2.20081","article-title":"Reused Poultry Litter Microbiome with Competitive Exclusion Potential against Salmonella Heidelberg","volume":"49","author":"Bucher","year":"2020","journal-title":"J. Environ. Qual."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.3382\/ps.0621741","article-title":"Influence of Litter Type and Size on Broiler Performance","volume":"62","author":"Malone","year":"1983","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"995","DOI":"10.3382\/ps.0340995","article-title":"The Absorption and Excretion of Orally Administered Nicarbazin by Chickens","volume":"34","author":"Porter","year":"1955","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1126\/science.6635662","article-title":"Nicarbazin Complex Yields Dinitrocarbanilide as Ultrafine Crystals with Improved Anticoccidial Activity","volume":"222","author":"Rogers","year":"1983","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1093\/jaoacint\/qsac011","article-title":"Determination and Identification of Nicarbazin, Measured as 4,4\u2032-Dinitrocarbanilide (DNC), in Chicken Tissues by Liquid Chromatography with Tandem Mass Spectrometry: Final Action 2013.07","volume":"105","author":"Brunelle","year":"2022","journal-title":"J. AOAC Int."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Martins, R.R., Silva, L.J.G., Pereira, A.M.P.T., Esteves, A., Duarte, S.C., and Pena, A. (2022). Coccidiostats and Poultry: A Comprehensive Review and Current Legislation. Foods, 11.","DOI":"10.3390\/foods11182738"},{"key":"ref_30","unstructured":"BRASIL. ANVISA\u2014National Health Surveillance Agency (2022, October 27). Normative Instruction Number 117, from February, 2nd 2022. Nicarbazin Active Ingredient Change for Authorized Use, Available online: https:\/\/pesquisa.in.gov.br\/imprensa\/jsp\/visualiza\/index.jsp?jornal=515&pagina=147&data=09\/02\/2022."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1574-6968.2002.tb11306.x","article-title":"Metabolism of Diphenylurea by a Marinobacter Sp. Isolated from a Contaminated Ephemeral Stream Bed in the Negev Desert","volume":"213","author":"Arbeli","year":"2002","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"121944","DOI":"10.1016\/j.jhazmat.2019.121944","article-title":"Fate, Risk and Removal of Triclocarban: A Critical Review","volume":"387","author":"Yun","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.2527\/jas1975.4141212x","article-title":"Medicinal Drug Residues in Broiler Litter and Tissues from Cattle Fed Litter","volume":"41","author":"Webb","year":"1975","journal-title":"J. Anim. Sci."},{"key":"ref_34","first-page":"83","article-title":"Nicarbazin","volume":"Volume 41\/11","author":"Wells","year":"1999","journal-title":"Residues of some Veterinary Drugs in Animals and Foods, Proceedings of the Fiftieth Meeting of the Joint FAO\/WHO Expert Committee on Food Additives"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Lima, A.L.d., Barreto, F., Rau, R.B., Silva, G.R.d., Lara, L.J.C., Figueiredo, T.C.d., Assis, D.C.S.d., Can\u00e7ado, S.d.V., Resende, G., and Lara, C. (2017). Determination of the Residue Levels of Nicarbazin and Combination Nicarbazin-Narasin in Broiler Chickens after Oral Administration. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181755"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8391","DOI":"10.1021\/acs.jafc.8b02370","article-title":"Degradation of 4,4-Dinitrocarbanilide in Chicken Breast by Thermal Processing","volume":"66","author":"Bacila","year":"2018","journal-title":"J. Agric. Food Chem."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3107\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:14:13Z","timestamp":1760145253000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":36,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ani12223107"],"URL":"https:\/\/doi.org\/10.3390\/ani12223107","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]}}}