{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:09:36Z","timestamp":1762074576432,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T00:00:00Z","timestamp":1668124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EMBRAPA","award":["06\/2018 FAPEG"],"award-info":[{"award-number":["06\/2018 FAPEG"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The present study aimed to determine whether cumulus cells (CC) biopsy, acquired before or after in vitro maturation (IVM), presents similar gene expression pattern and if would compromises oocyte quality. First, immature cumulus oocyte complexes (COCs) were distributed: (1) maturated in groups (control); (2) individually maturated, but not biopsied; (3) subjected to CC biopsy before maturation and individually matured; (4) individually matured and submitted to CC biopsy after maturation; (5) individually matured and CC biopsied before and after maturation. Secondly, candidate genes, described as potential markers of COCs quality, were quantified by RT-qPCR in CCs before and after IVM. After in vitro fertilization (IVF), zygotes were tracked and sorted regarding their developmental potential: fully developed to embryo, cleaved and arrested, and not-cleaved. The COC\u2019s biopsy negatively affects embryo development (p &lt; 0.05), blastocyst cell number (p &lt; 0.05), and apoptotic cell ratio (p &lt; 0.05), both before and after IVM. The PTGS2, LUM, ALCAM, FSHR, PGR, SERPINE2, HAS2, and PDRX3 genes were differentially expressed (p &lt; 0.05) on matured CCs. Only PGR gene (p = 0.04) was under-expressed on matured CCs on Not-Cleaved group. The SERPINE2 gene was overexpressed (p = 0.01) in the Cleaved group on immature CCs. In summary, none of the selected gene studies can accurately predict COC\u2019s fate after fertilization.<\/jats:p>","DOI":"10.3390\/ani12223113","type":"journal-article","created":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T01:39:54Z","timestamp":1668130794000},"page":"3113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Using Cumulus Cell Biopsy as a Non-Invasive Tool to Access the Quality of Bovine Oocytes: How Informative Are They?"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5507-3923","authenticated-orcid":false,"given":"Jos\u00e9 Felipe Warmling","family":"Spr\u00edcigo","sequence":"first","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s, UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-2490","authenticated-orcid":false,"given":"Ana Luiza Silva","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Centro Universit\u00e1rio Luterano de Palmas, CEULP, Palmas 77019-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7272-468X","authenticated-orcid":false,"given":"Andrielle Thainar Mendes","family":"Cunha","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio de Desenvolvimento do Centro Oeste, Luzi\u00e2nia 72852-580, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Animal, Universidade de Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4818-1202","authenticated-orcid":false,"given":"Ligiane de Oliveira","family":"Leme","sequence":"additional","affiliation":[{"name":"Embrapa Recursos Gen\u00e9ticos e Biotecnologia, Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Bras\u00edlia 70770-917, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Coura","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s, UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-9079","authenticated-orcid":false,"given":"Maur\u00edcio Machaim","family":"Franco","sequence":"additional","affiliation":[{"name":"Embrapa Recursos Gen\u00e9ticos e Biotecnologia, Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Bras\u00edlia 70770-917, Brazil"},{"name":"Institute of Genetics and Biochemistry, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-32, Brazil"},{"name":"School of Veterinary Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38410-337, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margot Alves Nunes","family":"Dode","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Animal, Universidade de Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"},{"name":"Embrapa Recursos Gen\u00e9ticos e Biotecnologia, Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Bras\u00edlia 70770-917, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1002\/mrd.1153","article-title":"Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst quality","volume":"61","author":"Rizos","year":"2002","journal-title":"Mol. 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