{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:10:03Z","timestamp":1762074603853,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:00:00Z","timestamp":1668384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICAR-IVRI"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Understanding the molecular cross-talk between the embryo and uterine endometrium is crucial for the improvement of IVF outcomes. The present work was undertaken to investigate the effect of pre-implantation embryo on the expression profile of immune-related genes in uterine epithelial cells (UECs) and PBMCs in buffalo. UECs were isolated from slaughterhouse-derived non-gravid uteri, cultured ex vivo and characterized, and buffalo embryos were produced in vitro from slaughterhouse-derived ovaries. Embryos co-cultured with steroid-treated UECs significantly stimulated (p &lt; 0.05) the relative mRNA abundance of PTGS2, ISG15, OAS1, MX2, IFNAR1 and IFNAR2 in UECs while they significantly suppressed the mRNA expression of NFk\u03b2IA, NFk\u03b22, TNF\u03b1 and IL1B, with no significant change in TGF\u03b21 and IL10 in the co-culture of embryos with UECs. In vitro treatment of PBMCs with conditioned media (CM) derived from embryos as well as UEC\u2013embryo co-culture upregulated the mRNA abundance of ISG15, TGF\u03b21, PTGS2OAS1, MX2 and STAT1 while it downregulated IL17 and TNF\u03b1 expression. The expression of IFNAR1 and IFNAR2 was elevated in PBMCs cultured in embryo-derived CM, but there was no significant change in PBMCs cultured in UEC\u2013embryo co-culture CM. Thus, it can be concluded that the developing embryo and its secretions modulate the expression of immune responses by inducing an anti-inflammatory action in uterine epithelial cells for acceptance of the semi-allogenic embryo in the uterus to sustain pregnancy in buffalo.<\/jats:p>","DOI":"10.3390\/ani12223138","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T02:31:15Z","timestamp":1668479475000},"page":"3138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Embryo\u2013Uterine Cross-Talk: Exploration of the Immunomodulatory Mechanism in Buffalo"],"prefix":"10.3390","volume":"12","author":[{"given":"Lakshmi Devi","family":"Huidrom","sequence":"first","affiliation":[{"name":"Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shital Nagargoje","family":"Dhanaji","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sriti","family":"Pandey","sequence":"additional","affiliation":[{"name":"Rewa Veterinary College, Nanaji Deshmukh Veterinary Science University, Jabalpur 482001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2142-3024","authenticated-orcid":false,"given":"Vikash","family":"Chandra","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taru Sharma","family":"Gutulla","sequence":"additional","affiliation":[{"name":"National Institute of Animal Biotechnology, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4081\/ijas.2007.s2.119","article-title":"Embryonic mortality in buffalo cows","volume":"6","author":"Campanile","year":"2007","journal-title":"Ital. 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