{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:12:15Z","timestamp":1790194335601,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minister of Education and Science","award":["010\/RID\/2018\/19"],"award-info":[{"award-number":["010\/RID\/2018\/19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>One of the important problems in poultry production is bacterial infections and increasing resistance to antibiotics. The increasing incidence of multidrug-resistant bacteria is a major challenge for physicians and veterinarians and considerably limits treatment options. This study was undertaken in order to assess the prevalence and antibiotic resistance of Klebsiella spp. strains isolated from turkeys sampled from 2019 to 2022 in Poland. The material for study consisted of clinical samples taken during routine monitoring and microbiological identification testing at commercial poultry farms. From all 507 isolates of Klebsiella, 95% were identified by MALDI-TOF (Matrix-Assisted Laser Desorption - Ionisation-Time of Flight) as Klebsiella pneumonia, 2% were Klebsiella\u00a0oxytoca, 2% Klebsiella\u00a0variicola, or unidentified (1%). All isolated Klebsiella strains were tested for antimicrobial susceptibility by disk diffusion. The results of our study indicated that colistin, neomycin, florfenicol and amoxicillin\/clavulanic acid were the most effective against the Klebsiella spp. isolated from turkeys. In addition, the results show a decrease in the number of multi-resistant Klebsiella spp. strains between 2019 and 2021.<\/jats:p>","DOI":"10.3390\/ani12223157","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T02:33:48Z","timestamp":1668566028000},"page":"3157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identification and Antimicrobial Resistance in Klebsiella spp. Isolates from Turkeys in Poland between 2019 and 2022"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8572-9014","authenticated-orcid":false,"given":"Joanna","family":"Kowalczyk","sequence":"first","affiliation":[{"name":"Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilona","family":"Czokaj\u0142o","sequence":"additional","affiliation":[{"name":"SLW Biolab Veterinary Laboratory, ul. Grunwaldzka 62, 14-100 Ostr\u00f3da, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Ga\u0144ko","sequence":"additional","affiliation":[{"name":"SLW Biolab Veterinary Laboratory, ul. Grunwaldzka 62, 14-100 Ostr\u00f3da, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3288-0789","authenticated-orcid":false,"given":"Marcin","family":"\u015amia\u0142ek","sequence":"additional","affiliation":[{"name":"Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland"},{"name":"SLW Biolab Veterinary Laboratory, ul. Grunwaldzka 62, 14-100 Ostr\u00f3da, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Koncicki","sequence":"additional","affiliation":[{"name":"Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"ref_1","unstructured":"European Medicines Agency (2022, October 03). European Surveillance of Veterinary Antimicrobial Consumption, 2021. \u2018Sales of Veterinary Antimicrobial Agents in 31 European Countries in 2019 and 2020\u2019. (EMA\/58183\/2021). Available online: https:\/\/www.ema.europa.eu\/en\/documents\/report\/sales-veterinary-antimicrobial-agents-31-european-countries-2019-2020-trends-2010-2020-eleventh_en.pdf."},{"key":"ref_2","unstructured":"Quinn, P.J., Markey, B.K., Leonard, F.C., FitzPatrick, E.S., and Fanning, S. (2016). Concise Review of Veterinary Microbiology, Wiley Blackwell. [2nd ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.3389\/fcimb.2018.00004","article-title":"Colonization, infection, and the accessory genome of Klebsiella pneumoniae","volume":"8","author":"Martin","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1186\/s12879-017-2760-7","article-title":"Whole genome sequencing for the molecular characterization of carbapenem resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012\u20132014","volume":"17","author":"Rimoldi","year":"2017","journal-title":"BMC Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12941-019-0343-8","article-title":"Klebsiella pneumoniae: An increasing threat to public health","volume":"19","author":"Effah","year":"2020","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/femsre\/fux013","article-title":"Klebsiella pneumoniae: A major worldwide source and shuttle for antibiotic resistance","volume":"41","author":"Kondratyeva","year":"2017","journal-title":"FEMS Micro. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors","volume":"11","author":"Podschun","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","unstructured":"Koncicki, A., Stenzel, T., Koncicka-\u015awiderska, K., Tyka\u0142owski, B., \u015amia\u0142ek, M., and Kowalczyk, J. (2022, October 16). Algorytmy diagnostyczno-terapeutyczne w przebiegu wybranych bakteryjnych chor\u00f3b indyk\u00f3w. Available online: https:\/\/academica.edu.pl\/reading\/readMeta?cid=90915165&uid=92924904."},{"key":"ref_9","first-page":"1668789","article-title":"Klebsiella pneumonia and Its Antibiotic Resistance: A Bibliometric Analysis","volume":"2020","author":"Li","year":"2022","journal-title":"BioMed Res. Int."},{"key":"ref_10","unstructured":"Sweeney, M.T., Diaz-Campos, D.V., Bowden, R., Fritsche, T.R., Hayes, J., Langston, C., Lubbers, B., Martin-Jimenez, T., Miller, C., and Pallotta, C. (2019). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Clinical and Laboratory Standards Institute. VET01."},{"key":"ref_11","first-page":"697","article-title":"Wykrywanie \u03b2-laktamaz o rozszerzonym zakresie dzia\u0142ania (ESBL) w izolatach klinicznych Klebsiella pneumoniae: Test dw\u00f3ch kr\u0105\u017ck\u00f3w i test ATB BLSE","volume":"32","author":"Gniadkowski","year":"1996","journal-title":"Diag. Laborator."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"194","DOI":"10.3382\/japr.2010-00168","article-title":"Probiotics and phytogenics for poultry: Myth or reality?","volume":"19","author":"Applegate","year":"2010","journal-title":"J. Appl. Poult. Res."},{"key":"ref_13","first-page":"21","article-title":"Phytogenic feed additives as an alternative to antibiotic growth promoters in broiler chickens","volume":"2","author":"Murugesan","year":"2015","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"452","DOI":"10.3389\/fvets.2019.00452","article-title":"Raising Animals Without Antibiotics: U.S. Producer and Veterinarian Experiences and Opinions","volume":"6","author":"Singer","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.aninu.2018.03.002","article-title":"Use of antibiotics in broiler production: Global impacts and alternatives","volume":"4","author":"Mehdi","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3389\/fmicb.2014.00282","article-title":"Antibiotics in canadian poultry productions and anticipated alternatives","volume":"5","author":"Diarra","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"95","DOI":"10.14720\/aas.2009.94.2.14834","article-title":"Use of herbs and spices and their extracts in animal nutrition","volume":"94","author":"Frankic","year":"2009","journal-title":"Acta Agric. Slov."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.livsci.2015.10.001","article-title":"Phytoncide, phytogenic feed additive as an alternative to conventional antibiotics, improved growth performance and decreased excreta gas emission without adverse effect on meat quality in broiler chickens","volume":"181","author":"Li","year":"2015","journal-title":"Livest. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.3389\/fmicb.2018.02302","article-title":"ESBL-Producing Klebsiella pneumoniae in the Broiler Production Chain and the First Description of ST3128","volume":"3","author":"Daehre","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1292\/jvms.11-0372","article-title":"Prevalence of extended-spectrum \u03b2-lactamase-producing Escherichia coli and Klebsiella pneumoniae in food-producing animals","volume":"74","author":"Hiroi","year":"2012","journal-title":"J. Vet. Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1089\/mdr.2016.0096","article-title":"Prevalence of CTX-M-Producing Klebsiella spp. in broiler, kuroiler, and indigenous poultry in West Bengal State, India","volume":"24","author":"Mahanti","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5431","DOI":"10.1128\/AEM.67.12.5431-5436.2001","article-title":"Prevalence of Campylobacter spp., Escherichia Coli, and Salmonella serovars in retail chicken, Turkey, pork, and beef from the greater Washington, dc, area","volume":"67","author":"Zhao","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Theocharidi, N.A., Balta, I., Houhoula, D., Tsantes, A.G., Lalliotis, G.P., Polydera, A.C., Stamatis, H., and Halvatsiotis, P. (2022). High Prevalence of Klebsiella pneumoniae in Greek Meat Products: Detection of Virulence and Antimicrobial Resistance Genes by Molecular Techniques. Foods, 11.","DOI":"10.3390\/foods11050708"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.4315\/JFP-19-520","article-title":"Foodborne Klebsiella pneumoniae: Virulence Potential, Antibiotic Resistance, and Risks to Food Safety","volume":"83","author":"Hartantyo","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"39","DOI":"10.5114\/aoms.2012.27278","article-title":"Antimicrobial-resistant Klebsiella species isolated from free-range chicken samples in an informal settlement","volume":"8","author":"Fielding","year":"2012","journal-title":"Arch. Med. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.fm.2018.09.010","article-title":"Contamination of chicken meat with extended-spectrum beta-lactamase producing-Klebsiella pneumoniae and Escherichia coli during scalding and defeathering of broiler carcasses","volume":"77","author":"Projahn","year":"2019","journal-title":"Food Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ijfoodmicro.2016.06.001","article-title":"Characterization of antimicrobial resistance in Klebsiella species isolated from chicken broilers","volume":"232","author":"Wu","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"271","DOI":"10.26444\/aaem\/120927","article-title":"Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens\u2014Clinical submissions","volume":"28","author":"Majewski","year":"2021","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/jvim.12562","article-title":"ACVIM Consensus Statement on Therapeutic Antimicrobial Use in Animals and Antimicrobial Resistance","volume":"29","author":"Weese","year":"2015","journal-title":"J. Vet. Intern. Med."},{"key":"ref_30","unstructured":"Jankowski, J., and \u015amia\u0142ek, M. Personal communication."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3157\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:19:00Z","timestamp":1760145540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/22\/3157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,15]]},"references-count":30,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ani12223157"],"URL":"https:\/\/doi.org\/10.3390\/ani12223157","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,15]]}}}