{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:06:49Z","timestamp":1781114809845,"version":"3.54.1"},"reference-count":68,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172785"],"award-info":[{"award-number":["32172785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Cold stress will have a negative impact on animal welfare and health. In order to explore the effect of intermittent cold stimulation training on the cold resistance of broilers. Immune-related and intestinal barrier genes were detected before and after acute cold stress (ACS), aiming to find an optimal cold stimulation training method. A total of 240 1-day-old Ross broilers (Gallus) were divided into three groups (G1, G2, and G3), each with 5 replicates (16 chickens each replicate). The broilers of G1 were raised at normal temperature, while the broilers of G2 and G3 were treated with cold stimulation at 3 \u00b0C lower than the G1 for 3 h and 6 h from 15 to 35 d, respectively, at one-day intervals. At 50 d, the ambient temperature for all groups was reduced to 10 \u00b0C for six hours. The results demonstrated that before ACS, IL6, IL17, TLR21, and HSP40 mRNA levels in G3 were apparently down-regulated (p &lt; 0.05), while IL8 and Claudin-1 mRNA levels were significantly up-regulated compared with G1 (p &lt; 0.05). After ACS, IL2, IL6, and IL8 expression levels in G3 were lower than those in G2 (p &lt; 0.05). Compared to G2, Claudin-1, HSP90 mRNA levels, HSP40, and HSP70 protein levels were increased in G3 (p &lt; 0.05). The mRNA levels of TLR5, Mucin2, and Claudin-1 in G2 and IL6, IL8, and TLR4 in G3 were down-regulated after ACS, while IL2, IL6, and IL17 mRNA levels in G2 and HSP40 protein levels in G3 were up-regulated after ACS (p &lt; 0.05). Comprehensive investigation shows that cold stimulation at 3 \u00b0C lower than the normal feeding temperature for six hours at one day intervals can enhanced immune function and maintain the stability of intestinal barrier function to lessen the adverse effects on ACS in broilers.<\/jats:p>","DOI":"10.3390\/ani12233260","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T02:25:36Z","timestamp":1669256736000},"page":"3260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of Acute Cold Stress after Intermittent Cold Stimulation on Immune-Related Molecules, Intestinal Barrier Genes, and Heat Shock Proteins in Broiler Ileum"],"prefix":"10.3390","volume":"12","author":[{"given":"Xiaotao","family":"Liu","sequence":"first","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Xing","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rixin","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7736-0333","authenticated-orcid":false,"given":"Jianhong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Bao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/j.psj.2019.10.024","article-title":"Short cold exposures during incubation and postnatal cold temperature affect performance, breast meat quality, and welfare parameters in broiler chickens","volume":"99","author":"Nyuiadzi","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.5713\/ajas.16.0957","article-title":"Performance differences of Rhode Island Red, Bashang Long-tail Chicken, and their reciprocal crossbreds under natural cold stress","volume":"30","author":"Xie","year":"2017","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"139","DOI":"10.5958\/j.0976-0555.48.2.030","article-title":"Impact of cold stress on blood biochemical and immune status in male and female vanaraja chickens","volume":"48","author":"Aarif","year":"2014","journal-title":"Indian J. Anim. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1080\/03079457.2015.1083094","article-title":"The effect of cold stress on the pathogenesis of necrotic enteritis in broiler chicks","volume":"44","author":"Tsiouris","year":"2015","journal-title":"Avian Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"101294","DOI":"10.1016\/j.psj.2021.101294","article-title":"Warm perches: A novel approach for reducing cold stress effect on production, plasma hormones, and immunity in laying hens","volume":"100","author":"Hu","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1080\/00071669308417573","article-title":"Effect of cold stress on performance and immune responses of Bedouin and White Leghorn hens","volume":"34","author":"Spinu","year":"1993","journal-title":"Br. Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7198","DOI":"10.1002\/jcp.27473","article-title":"Pre-cold acclimation improves the immune function of trachea and resistance to cold stress in broilers","volume":"234","author":"Su","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Liu, Y.H., Xue, G., Li, S., Fu, Y.J., Yin, J.W., Zhang, R.X., and Li, J.H. (2020). Effect of Intermittent and Mild Cold Stimulation on the Immune Function of Bursa in Broilers. Animals, 10.","DOI":"10.3390\/ani10081275"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101492","DOI":"10.1016\/j.psj.2021.101492","article-title":"Intermittent mild cold stimulation improves the immunity and cold resistance of spleens in broilers","volume":"100","author":"Xue","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.livsci.2017.08.004","article-title":"Effects of intermittent cold stimulation on antioxidant capacity and mRNA expression in broilers","volume":"204","author":"Li","year":"2017","journal-title":"Livest. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0306-4565(02)00025-6","article-title":"Early age cold conditioning in broiler chickens (Gallus domesticus): Thermotolerance and growth responses","volume":"27","author":"Shinder","year":"2002","journal-title":"J. Therm. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3382\/ps\/pez570","article-title":"Proper cold stimulation starting at an earlier age can enhance immunity and improve adaptability to cold stress in broilers","volume":"99","author":"Su","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Nguyen, P., Greene, E., Ishola, P., Huff, G., Donoghue, A., Bottje, W., and Dridi, S. (2015). Chronic Mild Cold Conditioning Modulates the Expression of Hypothalamic Neuropeptide and Intermediary Metabolic-Related Genes and Improves Growth Performances in Young Chicks. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0142319"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.3390\/polym3042010","article-title":"Role of Antimicrobial Peptides in Inflammatory Bowel Disease","volume":"3","author":"Otte","year":"2011","journal-title":"Polymers"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.cbi.2015.11.010","article-title":"Pathway underlying small intestine apoptosis by dietary nickel chloride in broiler chickens","volume":"24","author":"Wu","year":"2016","journal-title":"Chem. Biol. Interact."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.3382\/ps\/pew074","article-title":"Effects of early life dextran sulfate sodium administration on pathology and immune response in broilers and layers","volume":"95","author":"Simon","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_17","first-page":"612","article-title":"Multisociety Consensus Quality Improvement Revised Consensus Statement for Endovascular Therapy of Acute Ischemic Stroke","volume":"13","author":"Sacks","year":"2018","journal-title":"Int. J. Stroke"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101282","DOI":"10.1016\/j.psj.2021.101282","article-title":"Research Note: Immunocompetent cells in blood and intestine after administration of Lacto-Immuno-Vital in drinking water of broiler chickens","volume":"100","author":"Levkut","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5424\/sjar\/2018162-12753","article-title":"Comparison of growth performance and immune responses of broiler chicks reared under heat stress, cold stress and thermoneutral conditions","volume":"16","author":"Olfati","year":"2018","journal-title":"Span. J. Agric. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.3389\/fphys.2018.01589","article-title":"Effects of Acute Cold Stress after Long-Term Cold Stimulation on Antioxidant Status, Heat Shock Proteins, Inflammation and Immune Cytokines in Broiler Heart","volume":"9","author":"Wei","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4228","DOI":"10.3382\/ps\/pey308","article-title":"Effects of prior cold stimulation on inflammatory and immune regulation in ileum of cold-stressed broilers","volume":"97","author":"Su","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_22","first-page":"A7394","article-title":"Dynamics of Cell Pattern and Profile of Bronchial Cytokines in Response to Acute Cold Exposure in Asthma Patients with Cold Airway Hyperresponsiveness","volume":"201","author":"Perelman","year":"2020","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1365-2249.2010.04156.x","article-title":"The effect of adrenomedullin and cold stress on interleukin-6 levels in some rat tissues","volume":"161","author":"Yildirim","year":"2010","journal-title":"Clin. Exp. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s10571-017-0531-z","article-title":"Prior Repeated Stress Attenuates Cold-Induced Immunomodulation Associated with \"Browning\" in Mesenteric Fat of Rats","volume":"38","author":"Vargovic","year":"2018","journal-title":"Cell. Mol. Neurobiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern Recognition.n Receptors and Inflammation","volume":"140","author":"Takeuchi","year":"2010","journal-title":"Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1093\/molbev\/msy119","article-title":"Toll-like Receptor Evolution in Birds: Gene Duplication, Pseudogenization, and Diversifying Selection","volume":"35","author":"Burt","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.lfs.2017.11.047","article-title":"Lycium barbarum polysaccharides improve CCl 4 -induced liver fibrosis, inflammatory response and TLRs\/NF-kB signaling pathway expression in wistar rats","volume":"192","author":"Gan","year":"2018","journal-title":"Life Sci."},{"key":"ref_28","first-page":"1054","article-title":"The role and difference of TLR2 and TLR4 in rhabdomyolysis induced acute kidney injury in mice","volume":"11","author":"He","year":"2018","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1080\/09291016.2018.1464638","article-title":"Summer season induced heat stress impact on the expression patterns of different toll-like receptor genes in Malabari goats","volume":"50","author":"Vandana","year":"2019","journal-title":"Biol. Rhythm Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, S., Li, J.H., Liu, Y.H., Li, C., Zhang, R.X., and Bao, J. (2020). Effects of Intermittent Mild Cold Stimulation on mRNA Expression of Immunoglobulins, Cytokines, and Toll-Like Receptors in the Small Intestine of Broilers. Molecules, 10.","DOI":"10.3390\/ani10091492"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00441-010-1026-0","article-title":"Avian toll-like receptors","volume":"343","author":"Brownlie","year":"2011","journal-title":"Cell Tissue Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"357","DOI":"10.3906\/vet-1609-58","article-title":"Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens","volume":"41","author":"Theerawatanasirikul","year":"2017","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"183339","DOI":"10.1016\/j.bbamem.2020.183339","article-title":"Ruffles and spikes: Control of tight junction morphology and permeability by claudins","volume":"1862","author":"Lynn","year":"2020","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.amjms.2019.10.006","article-title":"The Prognostic Value of Endotoxemia and Intestinal Barrier Biomarker ZO-1 in Bacteremic Sepsis","volume":"359","author":"Assimakopoulos","year":"2019","journal-title":"Am. J. Med. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100138","DOI":"10.1016\/j.animal.2020.100138","article-title":"Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers","volume":"15","author":"Zhou","year":"2021","journal-title":"Animal"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.ajpath.2021.02.003","article-title":"Lactobacillus acidophilus Induces a Strain-specific and Toll-like Receptor 2-Dependent Enhancement of Intestinal Epithelial Tight Junction Barrier and Protection Against Intestinal Inflammation","volume":"191","author":"Nighot","year":"2021","journal-title":"Am. J. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"172","DOI":"10.3382\/ps\/peu064","article-title":"Development of intestinal mucin 2, IgA, and polymeric Ig receptor expressions in broiler chickens and Pekin ducks","volume":"94","author":"Zhang","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e00905-16","DOI":"10.1128\/IAI.00905-16","article-title":"MUC2 Mucin and Butyrate Contribute to the Synthesis of the Antimicrobial Peptide Cathelicidin in Response to Entamoeba histolytica- and Dextran Sodium Sulfate-Induced Colitis","volume":"85","author":"Cobo","year":"2017","journal-title":"Infect. Immun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.3382\/ps\/pey229","article-title":"Heat exposure affects jejunal tight junction remodeling independently of adenosine monophosphate-activated protein kinase in 9-day-old broiler chicks","volume":"97","author":"Uerlings","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/03079457.2018.1524574","article-title":"Heat stress as a predisposing factor for necrotic enteritis in broiler chicks","volume":"47","author":"Tsiouris","year":"2018","journal-title":"Avian Pathol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4502","DOI":"10.1038\/s41598-017-04755-w","article-title":"Chronic stress and intestinal barrier dysfunction: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter","volume":"7","author":"Zheng","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1571406","DOI":"10.1155\/2018\/1571406","article-title":"Role of MAPKs in HSP70's Protection against Heat Stress-Induced Injury in Rat Small Intestine","volume":"2018","author":"Hao","year":"2018","journal-title":"Biomed Res. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"919","DOI":"10.3389\/fphys.2017.00919","article-title":"Effect of Morinda citrifolia (Noni)-Enriched Diet on Hepatic Heat Shock Protein and Lipid Metabolism-Related Genes in Heat Stressed Broiler Chickens","volume":"27","author":"Flees","year":"2017","journal-title":"Front. Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3382\/ps\/pew402","article-title":"Hsp70 expression induced by Co-Enzyme Q10 protected chicken myocardial cells from damage and apoptosis under in vitro heat stress","volume":"96","author":"Xu","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.rvsc.2018.11.027","article-title":"Impact of strain and duration of thermal stress on carcass yield, metabolic hormones, immunological indices and the expression of HSP90 and Myogenin genes in broilers","volume":"122","author":"Zaglool","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110078","DOI":"10.1016\/j.ecoenv.2019.110078","article-title":"Ammonia inhalation impaired immune function and mitochondrial integrity in the broilers bursa of fabricius: Implication of oxidative stress and apoptosis","volume":"190","author":"Shah","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"111413","DOI":"10.1016\/j.ecoenv.2020.111413","article-title":"The effect of ammonia exposure on energy metabolism and mitochondrial dynamic proteins in chicken thymus: Through oxidative stress, apoptosis, and autophagy","volume":"206","author":"Shah","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.rvsc.2013.01.021","article-title":"Effects of cold stress on mRNA expression of immunoglobulin and cytokine in the small intestine of broilers","volume":"95","author":"Zhao","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.3382\/ps.0571430","article-title":"Influence of temperature on the immune response of birds","volume":"57","author":"Thaxton","year":"1978","journal-title":"Poult. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1080\/15321819.2018.1500375","article-title":"Use of chicken immunoglobulin Y in general virology","volume":"39","author":"Lanzarini","year":"2018","journal-title":"J. Immunoass. Immunochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0889-1591(92)90059-W","article-title":"Phentolamine but not propranolol blocks the immunopotentiating effect of cold stress on antigen-specific IgM production in mice orally immunized with sheep red blood cells","volume":"6","author":"Carr","year":"1992","journal-title":"Brain Behav. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/S1054-3589(08)60818-2","article-title":"The role of the sympathetic nervous system in the modulation of immune responses","volume":"42","author":"Felten","year":"1998","journal-title":"Advan Pharmacol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.3382\/ps\/pey499","article-title":"Effects of pre-hatch thermal manipulation and post-hatch acute heat stress on the mRNA expression of interleukin-6 and genes involved in its induction pathways in 2 broiler chicken breeds","volume":"98","author":"Saleh","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.intimp.2014.12.027","article-title":"Zerumbone enhances the Th1 response and ameliorates ovalbumin-induced Th2 responses and airway inflammation in mice","volume":"24","author":"Shieh","year":"2015","journal-title":"Int. Immunopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.3923\/javaa.2012.1538.1545","article-title":"Different Duration of Cold Stress Enhances Pro-Inflammatory Cytokines Profile and Alterations of Th1 and Th2 Type Cytokines Secretion in Serum of Wistar Rats","volume":"11","author":"Guo","year":"2012","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1152\/jappl.1999.87.2.699","article-title":"Immune changes in humans during cold exposure: Effects of prior heating and exercise","volume":"87","author":"Brenner","year":"1999","journal-title":"J. Appl. Physiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"878","DOI":"10.2307\/3285825","article-title":"Cold stress-induced modulation of inflammatory responses and intracerebral cytokine mRNA expression in acute murine toxoplasmosis","volume":"85","author":"Monroy","year":"1999","journal-title":"J. Parasitol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"153177","DOI":"10.1016\/j.tox.2022.153177","article-title":"Toll-like receptors in the mechanism of tributyltin-induced production of pro-inflammatory cytokines, IL-1\u03b2 and IL-6","volume":"30","author":"Alcala","year":"2022","journal-title":"Toxicology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"850394","DOI":"10.3389\/fopht.2022.850394","article-title":"Toll-like Receptor Signalling Pathways and the Pathogenesis of Retinal Diseases","volume":"2","author":"Titi","year":"2022","journal-title":"Front. Ophthalmol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s11684-010-0600-x","article-title":"Toll-like receptors in innate immunity and infectious diseases","volume":"4","author":"Wu","year":"2010","journal-title":"Front. Med. China"},{"key":"ref_61","first-page":"19","article-title":"Heat stress decreases expression of the cytokines, avian beta-defensins 4 and 6 and Toll-like receptor 2 in broiler chickens infected with Salmonella Enteritidis","volume":"40","author":"Calefi","year":"2017","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s13205-015-0306-5","article-title":"Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla)","volume":"5","author":"Basu","year":"2015","journal-title":"3 Biotech"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Varasteh, S., Braber, S., Akbari, P., Garssen, J., and Fink-Gremmels, J. (2017). Differences in Susceptibility to Heat Stress along the Chicken Intestine and the Protective Effects of Galacto-Oligosaccharides. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0138975"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.pharmthera.2003.11.004","article-title":"Heat shock proteins in the regulation of apoptosis: New strategies in tumor therapy: A comprehensive review","volume":"101","author":"Sreedhar","year":"2004","journal-title":"Pharmacol. Ther."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/s12011-015-0282-y","article-title":"Selenium Deficiency Downregulates Selenoproteins and Suppresses Immune Function in Chicken Thymus","volume":"167","author":"Khoso","year":"2015","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.lfs.2010.02.010","article-title":"Overexpression of a 60-kDa heat shock protein enhances cytoprotective function of small intestinal epithelial cells","volume":"86","author":"Takada","year":"2010","journal-title":"Life Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.canlet.2012.06.003","article-title":"Inhibition of HSP70: A challenging anti-cancer strategy","volume":"325","author":"Goloudina","year":"2012","journal-title":"Cancer Lett."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.1161\/ATVBAHA.107.151274","article-title":"Induction of oral tolerance to HSP60 or an HSP60-peptide activates T cell regulation and reduces atherosclerosis","volume":"27","author":"Puijvelde","year":"2007","journal-title":"Arterioscler. Thromb. Vasc. 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