{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:23:52Z","timestamp":1762079032939,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"United States Department of Agriculture","doi-asserted-by":"publisher","award":["2018-67001-28266"],"award-info":[{"award-number":["2018-67001-28266"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fraxa Research Foundation","award":["2018-67001-28266"],"award-info":[{"award-number":["2018-67001-28266"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Animal husbandry conditions, including rodent diet, constitute an example highlighting the importance of reporting experimental variables to enhance scientific rigor. In the present study, we examine the effects of three common rodent diets including two chows (Purina 5015 and Teklad 2019) and one purified ingredient diet (AIN-76A) on growth anthropometrics (body weight), behavior (nest building, actigraphy, passive avoidance) and blood biomarkers (ketones, glucose, amino acid profiles) in male and female C57BL\/6J mice. We find increased body weight in response to the chows compared to purified ingredient diet albeit selectively in male mice. We did not find significantly altered behavior in female or male wild type C57BL\/6J mice. However, amino acid profiles changed as an effect of sex and diet. These data contribute to a growing body of knowledge indicating that rodent diet impacts experimental outcomes and needs to be considered in study design and reporting.<\/jats:p>","DOI":"10.3390\/ani12243448","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T03:27:57Z","timestamp":1670383677000},"page":"3448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Improving Reproducibility to Enhance Scientific Rigor through Consideration of Mouse Diet"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3919-3279","authenticated-orcid":false,"given":"Cara J.","family":"Westmark","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Wisconsin, Madison, WI 53706, USA"},{"name":"Molecular Environmental Toxicology Center, University of Wisconsin, Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Brower","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice K.","family":"Held","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/nature11556","article-title":"A call for transparent reporting to optimize the predictive value of preclinical research","volume":"490","author":"Landis","year":"2012","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106891","DOI":"10.1016\/j.eplepsyres.2022.106891","article-title":"Spontaneous seizures in adult Fmr1 knockout mice: FVB.129P2-Pde6b(+)Tyr(c-ch)Fmr1(tm1Cgr)\/J","volume":"182","author":"Armstrong","year":"2022","journal-title":"Epilepsy Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/aur.168","article-title":"Modifying behavioral phenotypes in Fmr1KO mice: Genetic background differences reveal autistic-like responses","volume":"4","author":"Spencer","year":"2011","journal-title":"Autism Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"797","DOI":"10.3233\/JAD-2012-121426","article-title":"Soy-based diet exacerbates seizures in mouse models of neurological disease","volume":"33","author":"Westmark","year":"2013","journal-title":"J. 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