{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:18Z","timestamp":1787027478177,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Project of the Education Department of Guangdong Province, China","award":["2022KTSCX124"],"award-info":[{"award-number":["2022KTSCX124"]}]},{"name":"Research Project of the Education Department of Guangdong Province, China","award":["2021ZYTS34"],"award-info":[{"award-number":["2021ZYTS34"]}]},{"name":"Research Project of the Education Department of Guangdong Province, China","award":["2020KJ137"],"award-info":[{"award-number":["2020KJ137"]}]},{"name":"Research Project of the Education Department of Guangdong Province, China","award":["2020ZDZX1004"],"award-info":[{"award-number":["2020ZDZX1004"]}]},{"name":"Postgraduate exploration foundation of Foshan University","award":["2022KTSCX124"],"award-info":[{"award-number":["2022KTSCX124"]}]},{"name":"Postgraduate exploration foundation of Foshan University","award":["2021ZYTS34"],"award-info":[{"award-number":["2021ZYTS34"]}]},{"name":"Postgraduate exploration foundation of Foshan University","award":["2020KJ137"],"award-info":[{"award-number":["2020KJ137"]}]},{"name":"Postgraduate exploration foundation of Foshan University","award":["2020ZDZX1004"],"award-info":[{"award-number":["2020ZDZX1004"]}]},{"name":"Guangdong Province Modern Agricultural industry Technology system innovation team project","award":["2022KTSCX124"],"award-info":[{"award-number":["2022KTSCX124"]}]},{"name":"Guangdong Province Modern Agricultural industry Technology system innovation team project","award":["2021ZYTS34"],"award-info":[{"award-number":["2021ZYTS34"]}]},{"name":"Guangdong Province Modern Agricultural industry Technology system innovation team project","award":["2020KJ137"],"award-info":[{"award-number":["2020KJ137"]}]},{"name":"Guangdong Province Modern Agricultural industry Technology system innovation team project","award":["2020ZDZX1004"],"award-info":[{"award-number":["2020ZDZX1004"]}]},{"name":"Key field project of the Education Department of Guangdong Province, China","award":["2022KTSCX124"],"award-info":[{"award-number":["2022KTSCX124"]}]},{"name":"Key field project of the Education Department of Guangdong Province, China","award":["2021ZYTS34"],"award-info":[{"award-number":["2021ZYTS34"]}]},{"name":"Key field project of the Education Department of Guangdong Province, China","award":["2020KJ137"],"award-info":[{"award-number":["2020KJ137"]}]},{"name":"Key field project of the Education Department of Guangdong Province, China","award":["2020ZDZX1004"],"award-info":[{"award-number":["2020ZDZX1004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Duck plague (DP) is a highly contagious viral disease in ducks caused by the duck plague virus (DPV). The DPV, a member of Herpesviridae, poses a severe threat to the waterfowl farming industry worldwide. In this study, we reported a recent outbreak of DPV in domestic laying ducks at 310 days of age from southern China in December 2021. The gross lesion, histopathologic examination, molecular detection, and genetic characterization studies of DPV are described here. As a result, gross lesions such as an enlarged congestive spleen and liver were observed. Liver with vacuolar degeneration and small vacuoles and spleen with hemosiderosis were remarkable microscopic findings. Our results suggested that the liver had the highest viral load, followed by the trachea, pancreas, kidney, brain, spleen, and heart. In addition, DPV was successfully isolated in chicken embryo fibroblast cell culture and designated as DP-GD-305-21. The UL2, UL12, UL41, UL47, and LORF11 genes of DP-GD-305-21 shared a high nucleotide homology with the Chinese virulent (CHv) strain and the Chinese variant (CV) strain. In conclusion, this study reports the isolation and molecular characterization of DPV from a recent outbreak in southern China. Our results contributed to the understanding of the pathological and molecular characterization of currently circulating DPV in China.<\/jats:p>","DOI":"10.3390\/ani12243523","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T02:54:21Z","timestamp":1670986461000},"page":"3523","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021"],"prefix":"10.3390","volume":"12","author":[{"given":"Zhipeng","family":"Liang","sequence":"first","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyue","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congying","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhili","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Hou","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences\/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujian","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8790-1503","authenticated-orcid":false,"given":"Feng","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Foshan University, Foshan 528231, China"},{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2307\/1588237","article-title":"Duck plague on the American continent","volume":"12","author":"Leibovitz","year":"1968","journal-title":"Avian Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/03079450120118612","article-title":"Histopathological and ultrastructural changes associated with herpesvirus infection in waterfowl","volume":"31","author":"Salguero","year":"2002","journal-title":"Avian Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1637\/0005-2086-64.2.166","article-title":"Pathodynamics of Circulating Strains of Duck Enteritis Virus: A Step Forward to Understand Its Pathogenesis","volume":"64","author":"Sarmah","year":"2020","journal-title":"Avian Dis."},{"key":"ref_4","first-page":"308","article-title":"Duck plague outbreak in a Chara-Chemballi duck farm","volume":"20","author":"Pazhanivel","year":"2019","journal-title":"Iran. 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