{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T01:59:00Z","timestamp":1794275940329,"version":"4.9.0"},"reference-count":33,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014181","name":"Junta de Extremadura","doi-asserted-by":"publisher","award":["GR21085"],"award-info":[{"award-number":["GR21085"]}],"id":[{"id":"10.13039\/501100014181","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Regional Development Funds \u201cUna Manera de Hacer Europa\u201d","award":["GR21085"],"award-info":[{"award-number":["GR21085"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The transversus abdominis plane (TAP) block causes desensitization of the abdominal wall and peritoneum. Of all the approaches proposed to perform it, the two-injection-point TAP showed the best results in terms of the area reached by the anesthetic solution. However, to date, no clinical data exist. The aim of this study was to evaluate the intra- and postoperative analgesic efficacy of a two-injection-point TAP block in dogs undergoing laparoscopic ovariectomy. A total of 26 animals were assigned to receive general inhalation anesthesia (control group), and 26 dogs were assigned to general inhalation anesthesia combined with TAP block (TAP group). The ultrasound-guided TAP block was carried out with a subcostal and cranial-to-ilium injection per hemiabdomen. The end-tidal concentration of isoflurane (EtISO) was recorded at different moments during the surgery. Postoperative pain was assessed at different time points during the first 24 h after surgery. The control group required significantly higher EtISO concentration during the ovarian resection and showed higher postoperative pain scores than the TAP group. Fewer dogs in the TAP group required intra- or postoperative rescue analgesia. TAP block can be implemented to improve postoperative pain management after laparoscopy, reducing the dosage of the systemic drugs used and, hence, their possible side effects.<\/jats:p>","DOI":"10.3390\/ani12243556","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T02:16:41Z","timestamp":1671157001000},"page":"3556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of the Two-Point Ultrasound-Guided Transversus Abdominis Plane Block for Laparoscopic Canine Ovariectomy"],"prefix":"10.3390","volume":"12","author":[{"given":"Lorena","family":"Espadas-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Department of Animal Medicine, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7289-7852","authenticated-orcid":false,"given":"Jes\u00fas M.","family":"Us\u00f3n-Casa\u00fas","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Pastor-Sirvent","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3722-8503","authenticated-orcid":false,"given":"Massimo","family":"Santella","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2536-9480","authenticated-orcid":false,"given":"Javier","family":"Ezquerra-Calvo","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9118-1139","authenticated-orcid":false,"given":"Eva M.","family":"P\u00e9rez-Merino","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s\/n, 10003 C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1111\/j.1532-950X.2009.00572.x","article-title":"The effect of laparoscopic versus open ovariectomy on postsurgical activity in small dogs","volume":"38","author":"Culp","year":"2009","journal-title":"Vet. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"921","DOI":"10.2460\/javma.2005.227.921","article-title":"Duration, complications, stress, and pain of open ovariohysterectomy versus a simple method of laparoscopic-assisted ovariohysterectomy in dogs","volume":"227","author":"Devitt","year":"2005","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1532-950X.2004.04003.x","article-title":"Comparison of laparoscopic ovariohysterectomy and ovariohysterectomy in dogs","volume":"33","author":"Davidson","year":"2004","journal-title":"Vet. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Scott, J., Singh, A., and Valverde, A. (2020). Pneumoperitoneum in Veterinary Laparoscopy: A Review. Vet. Sci., 7.","DOI":"10.3390\/vetsci7020064"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/j.vaa.2018.06.014","article-title":"Two-point ultrasound-guided transversus abdominis plane injection in canine cadavers\u2014A pilot study","volume":"45","author":"Johnson","year":"2018","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cicirelli, V., Burgio, M., Lacalandra, G.M., and Aiudi, G.G. (2022). Local and Regional Anaesthetic Techniques in Canine Ovariectomy: A Review of the Literature and Technique Description. Animals, 12.","DOI":"10.3390\/ani12151920"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/vaa.12122","article-title":"Retrospective clinical evaluation of ultrasound guided transverse abdominis plane block in dogs undergoing mastectomy","volume":"41","author":"Portela","year":"2014","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/ahe.12279","article-title":"Anatomical Evaluation of the Thoracolumbar Nerves Related to the Transversus Abdominis Plane Block Technique in the Dog","volume":"46","year":"2017","journal-title":"Anat. Histol. Embryol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1213\/ane.0b013e3181ae0989","article-title":"An anatomical study of the transversus abdominis plane block: Location of the lumbar triangle of Petit and adjacent nerves","volume":"109","author":"Jankovic","year":"2009","journal-title":"Anesth. Analg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1467-2995.2011.00612.x","article-title":"Ultrasound-guided transversus abdominis plane block in the dog: An anatomical evaluation","volume":"38","author":"Schroeder","year":"2011","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/S0195-5616(08)70010-X","article-title":"Local and regional anesthesia","volume":"30","author":"Lemke","year":"2000","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_12","first-page":"2966","article-title":"Transversus abdominis plane block for postoperative analgesia after laparoscopic surgery: A systematic review and meta-analysis","volume":"7","author":"Zhao","year":"2014","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1097\/DCR.0000000000001292","article-title":"Randomized Clinical Trial Comparing Laparoscopic Versus Ultrasound-Guided Transversus Abdominis Plane Block in Minimally Invasive Colorectal Surgery","volume":"62","author":"Zaghiyan","year":"2019","journal-title":"Dis. Colon Rectum"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1007\/s00464-020-07558-9","article-title":"Laparoscopically guided transversus abdominis plane block offers better pain relief after laparoscopic cholecystectomy: Results of a triple blind randomized controlled trial","volume":"35","author":"Vindal","year":"2021","journal-title":"Surg. Endosc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1093\/bja\/aer069","article-title":"Ultrasound-guided transversus abdominis plane blocks for laparoscopic appendicectomy in children: A prospective randomized trial","volume":"106","author":"Sandeman","year":"2011","journal-title":"Br. J. Anaesth."},{"key":"ref_16","first-page":"487","article-title":"Transverses Abdominis Plane Block Versus Trocar Site Infiltration in Gynecologic Laparoscopy An Observational Study","volume":"8","author":"Qureshi","year":"2020","journal-title":"J. Med. Biomed. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"ajvr.22.03.0037","DOI":"10.2460\/ajvr.22.03.0037","article-title":"Transverse abdominis plane injection of bupivacaine with dexmedetomidine or a bupivacaine liposomal suspension yielded lower pain scores and requirement for rescue analgesia in a controlled, randomized trial in dogs undergoing elective ovariohysterectomy","volume":"83","author":"Campoy","year":"2022","journal-title":"Am. J. Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1031345","DOI":"10.3389\/fvets.2022.1031345","article-title":"Analgesic efficacy of ultrasound-guided transversus abdominis plane block in dogs undergoing ovariectomy","volume":"9","author":"Cavaco","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Paolini, A., Santoro, F., Bianchi, A., Collivignarelli, F., Vignoli, M., Scialanca, S., Parrillo, S., Falerno, I., De Bonis, A., and Rosto, M. (2022). Use of Transversus Abdominis Plane and Intercostal Blocks in Bitches Undergoing Laparoscopic Ovariectomy: A Randomized Controlled Trial. Vet. Sci., 9.","DOI":"10.3390\/vetsci9110604"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1532-950X.2012.00972.x","article-title":"Weight-based volume of injection influences cranial to caudal spread of local anesthetic solution in ultrasound-guided transversus abdominis plane blocks in canine cadavers","volume":"41","author":"Bruggink","year":"2012","journal-title":"Vet. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vaa.2020.09.005","article-title":"Comparison between two approaches for the transversus abdominis plane block in canine cadavers","volume":"48","author":"Romano","year":"2021","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1532-950X.2012.00961.x","article-title":"Right intercostal insertion of a Veress needle for laparoscopy in dogs","volume":"41","author":"Fiorbianco","year":"2012","journal-title":"Vet. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"885.e13","DOI":"10.1016\/j.vaa.2018.09.031","article-title":"Analgesic efficacy of ultrasound-guided transverse abdominis plane block in dogs undergoing ovariohysterectomy","volume":"45","author":"Santos","year":"2018","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.vaa.2018.02.003","article-title":"Continuous transverse abdominal plane block for analgesia in three dogs with abdominal pain","volume":"45","author":"Freitag","year":"2018","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.vaa.2020.10.001","article-title":"Evaluation of injection volumes for the transversus abdominis plane block in dog cadavers: A preliminary trial","volume":"48","author":"Freitag","year":"2021","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s12630-015-0338-7","article-title":"Evaluating the learning curve for the transversus abdominal plane block: A prospective observational study","volume":"62","author":"Vial","year":"2015","journal-title":"Can. J. Anaesth."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.vaa.2018.01.005","article-title":"Perioperative analgesic effects of an ultrasound-guided transversus abdominis plane block with a mixture of bupivacaine and lidocaine in cats undergoing ovariectomy","volume":"45","author":"Skouropoulou","year":"2018","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1590\/1678-5150-pvb-5007","article-title":"Combination of Transversus abdominis plane block and Serratus plane block anesthesia in dogs submitted to mastectomy","volume":"38","author":"Teixeira","year":"2018","journal-title":"Pesqui. Vet. Bras."},{"key":"ref_29","unstructured":"Cousins, M.J., Carr, D.B., Horlocker, T.T., and Bridenbaugh, P.O. (2009). Clinical Pharmacology of Local Anesthetics. Cousins & Bridenbaugh\u2019s Neural Blockade in Clinical Anesthesia and Pain Medicine, Lippincott Williams & Wilkins. [4th ed.]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.vaa.2018.06.012","article-title":"Comparison of intraperitoneal ropivacaine and bupivacaine for postoperative analgesia in dogs undergoing ovariohysterectomy","volume":"45","author":"Lambertini","year":"2018","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1292\/jvms.001279","article-title":"Relative nerve blocking properties of bupivacaine and ropivacaine in dogs undergoing brachial plexus block using a nerve stimulator","volume":"71","author":"Sakonju","year":"2009","journal-title":"J. Vet. Med. Sci."},{"key":"ref_32","first-page":"729","article-title":"Postoperative signs in 96 dogs undergoing soft tissue surgery","volume":"155","author":"Oksanen","year":"2004","journal-title":"Vet. Rec."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/vsu.13156","article-title":"Meloxicam vs. robenacoxib for postoperative pain management in dogs undergoing combined laparoscopic ovariectomy and laparoscopic-assisted gastropexy","volume":"48","author":"Bendinelli","year":"2019","journal-title":"Vet. Surg."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/24\/3556\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:42:15Z","timestamp":1760146935000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/24\/3556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":33,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ani12243556"],"URL":"https:\/\/doi.org\/10.3390\/ani12243556","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,15]]}}}