{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T07:28:45Z","timestamp":1777361325301,"version":"3.51.4"},"reference-count":61,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T00:00:00Z","timestamp":1672099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education and Science of the University of Agriculture in Krakow, Poland","award":["SUB-020002-D015"],"award-info":[{"award-number":["SUB-020002-D015"]}]},{"name":"Ministry of Education and Science of the University of Agriculture in Krakow, Poland","award":["SUB. 215-D202"],"award-info":[{"award-number":["SUB. 215-D202"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Roundup, the most popular herbicide in global agriculture, is regarded as an endocrine disruptor causing alterations of important hormones at the hypothalamic\u2212pituitary\u2212gonadal axis as well as impairment of gametogenesis. The whole pituitary glands of crucian carp (Carassius carassius) were incubated for 3 h in the medium containing Roundup (0-control, 1 and 10 ng\/mL). The level of luteinizing hormone (LH), and mRNA transcript abundance of kisspeptin (kiss-1) and its receptor (gpr54), were determined. The isolated ovarian fragments were incubated for 24 h in the presence of Roundup and the following effects on reproductive parameters were determined: the final oocyte maturation and ovulation, structural changes in follicles, secretion of 17,20\u03b2-progesterone (17,20\u03b2-P) as well as mRNA transcript abundance of the luteinizing hormone receptor (lhr), estrogen receptors (er\u03b1, er\u03b21, er\u03b22), and zona radiata (chorion) proteins (zp2 and zp3). Roundup inhibited final oocyte maturation and decreased the percentage of ovulated eggs, and furthermore, caused structural changes in the ovarian follicular components. There were no significant changes in the measured hormone levels and analyzed genes mRNA transcript abundance. Summing up, obtained results indicate that Roundup may adversely affect oocyte maturation and the quality of eggs, suggesting that exposure to this herbicide can lead to reproductive disorders in fish.<\/jats:p>","DOI":"10.3390\/ani13010105","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T05:28:24Z","timestamp":1672205304000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Roundup in the Reproduction of Crucian Carp (Carassius carassius): An In Vitro Effect on the Pituitary Gland and Ovary"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2466-8542","authenticated-orcid":false,"given":"Magdalena","family":"Socha","sequence":"first","affiliation":[{"name":"Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Szczygie\u0142","sequence":"additional","affiliation":[{"name":"Institute of Ichthyobiology and Aquaculture in Go\u0142ysz, Polish Academy of Sciences, Zaborze, Kalinowa 2, 43-520 Chybie, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0593-2256","authenticated-orcid":false,"given":"Jaros\u0142aw","family":"Chyb","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, University of Agriculture in Krakow, Al. Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2572-0034","authenticated-orcid":false,"given":"Ewa","family":"Dr\u0105g-Kozak","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, University of Agriculture in Krakow, Al. Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miros\u0142awa","family":"Soko\u0142owska-Miko\u0142ajczyk","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, University of Agriculture in Krakow, Al. Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El\u017cbieta","family":"Brzuska","sequence":"additional","affiliation":[{"name":"Institute of Ichthyobiology and Aquaculture in Go\u0142ysz, Polish Academy of Sciences, Zaborze, Kalinowa 2, 43-520 Chybie, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Pecio","sequence":"additional","affiliation":[{"name":"Department of Comparative Anatomy, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2972-1755","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Grzesiak","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5604\/01.3001.0015.9691","article-title":"Roundup in the aquatic environment and fish reproduction","volume":"18","author":"Suder","year":"2022","journal-title":"Anim. Sci. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1021\/es404258h","article-title":"Effects of glyphosate and its formulation, roundup, on reproduction in zebrafish (Danio rerio)","volume":"48","author":"Laing","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.aquatox.2014.07.006","article-title":"Effect of glyphosate on the sperm quality of zebrafish Danio rerio","volume":"155","author":"Lopes","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1007\/s10695-018-0498-9","article-title":"The effects of Roundup on gametes and early development of common carp (Cyprinus carpio L)","volume":"44","year":"2018","journal-title":"Fish Physiol. Biochem."},{"key":"ref_5","first-page":"59","article-title":"The effect of Roundup on gametes of grass carp Ctenopharyngodon idella Val.\u2014Preliminary research","volume":"16","year":"2020","journal-title":"Sci. Ann. Pol. Soc. Anim. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.marpolbul.2014.03.022","article-title":"Effect of glyphosate-based herbicide on early life stages of Java medaka (Oryzias javanicus): A potential tropical test fish","volume":"85","author":"Yusof","year":"2014","journal-title":"Mar. Pollut. Bull."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"8542","DOI":"10.1007\/s11356-017-1141-5","article-title":"Effects of glyphosate on early life stages: Comparison between Cyprinus carpio and Danio rerio","volume":"25","author":"Fiorino","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.cbpc.2019.04.007","article-title":"The effects of Roundup\u00ae in embryodevelopment and energy metabolism of the zebrafish (Danio rerio)","volume":"222","author":"Panetto","year":"2019","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.aquatox.2019.03.005","article-title":"Developmental and epigenetic effects of Roundup and glyphosate exposure on Japanese medaka (Oryzias latipes)","volume":"210","author":"Smith","year":"2019","journal-title":"Aquat. Toxicol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.theriogenology.2021.09.007","article-title":"The effect of Roundup on embryonic development, early foxr1 and hsp70 gene expression and hatching of common carp (Cyprinus carpio L.)","volume":"175","author":"Socha","year":"2021","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1002\/jat.2997","article-title":"Impact of glyphosate and glyphosate-based herbicides on the freshwater environment","volume":"34","author":"Annett","year":"2014","journal-title":"J. Appl. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1007\/s11356-016-7835-2","article-title":"Occurrence of the herbicide glyphosate and its metabolite AMPA in surface waters in Switzerland determined with on-line solid phase extraction LC-MS\/MS","volume":"24","author":"Poiger","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ijheh.2016.09.016","article-title":"Glyphosate in German adults\u2014Time trend (2001 to 2015) of humanexposure to a widely used herbicide","volume":"220","author":"Conrad","year":"2017","journal-title":"Int. J. Hyg. Environ. Health."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16451","DOI":"10.1007\/s11356-020-08183-8","article-title":"Low-dose Roundup induces developmental toxicity in bovine preimplantation embryos in vitro","volume":"27","author":"Cai","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"106710","DOI":"10.1016\/j.foodcont.2019.106710","article-title":"Glyphosate contamination in grains and foods: An overview","volume":"18","author":"Xu","year":"2019","journal-title":"Food Control."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1289\/ehp.00108769","article-title":"Roundup inhibits steroidogenesis by disrupting steroidogenic acute regulatory (StAR) protein expression","volume":"108","author":"Walsh","year":"2000","journal-title":"Environ. Health Perspect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1289\/ehp.7728","article-title":"Differential effects of glyphosate and roundup on human placental cells and aromatase","volume":"113","author":"Richard","year":"2005","journal-title":"Environ. Health Perspect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.tiv.2011.12.009","article-title":"A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro, and testosterone decrease at lower levels","volume":"26","author":"Clair","year":"2012","journal-title":"Toxicol. In Vitro"},{"key":"ref_19","first-page":"663","article-title":"Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression","volume":"86","author":"Romano","year":"2012","journal-title":"Reprod. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.etp.2017.04.007","article-title":"Reproductive toxicity of Roundup herbicide exposure in male albino rat","volume":"69","author":"Owagboriaye","year":"2017","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.chemosphere.2017.09.007","article-title":"Influence of a Roundup formulation on glyphosate effects on steroidogenesis and proliferation of bovine granulosa cells in vitro","volume":"188","author":"Parego","year":"2017","journal-title":"Chemosphere"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1080\/13813455.2017.1352606","article-title":"Subchronic exposure to kalach 360 SL-induced endocrine disruption and ovary damage in female rats","volume":"124","author":"Hamdaoui","year":"2018","journal-title":"Arch. Physiol. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"124435","DOI":"10.1016\/j.chemosphere.2019.124435","article-title":"The toxic effects and possible mechanisms of glyphosate on mouse oocytes","volume":"237","author":"Zhang","year":"2019","journal-title":"Chemosphere"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12007","DOI":"10.1038\/s41598-020-68813-6","article-title":"Glyphosate and its formulation Roundup impair pig oocyte maturation","volume":"10","author":"Spinaci","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.ygcen.2009.07.019","article-title":"Perspectives on fish gonadotropins and their receptors","volume":"165","author":"Bogerd","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.ecoenv.2017.06.002","article-title":"Exposure to E2 and EE2 environmental concentrations affect different components of the Brain-Pituitary-Gonadal axis in pejerrey fish (Odontesthes bonariensis)","volume":"144","author":"Miranda","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1210\/en.2017-00808","article-title":"Evolutionally conserved function of kisspeptin neuronal system is non reproductive regulation as revealed by no mammalian study","volume":"159","author":"Nakajo","year":"2018","journal-title":"Endocrinology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"113544","DOI":"10.1016\/j.ygcen.2020.113544","article-title":"Fish reproductive biology\u2014Reflecting on five decades of fundamental and translational research","volume":"300","author":"Zohar","year":"2021","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1440-169X.2008.01019.x","article-title":"Regulation of oocyte maturation in fish","volume":"50","author":"Nagahama","year":"2008","journal-title":"Dev. Growth Differ."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Biran, J., and Levavi-Sivan, B. (2018). Endocrine Control of Reproduction, Fish. Encyclopedia of Reproduction (Second Edition), Elsevier Inc.","DOI":"10.1016\/B978-0-12-809633-8.20579-7"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.aquaculture.2016.10.029","article-title":"Maternal investment in fish oocytes and eggs: The molecular cargo and its contributions to fertility and early development","volume":"472","author":"Lubzens","year":"2017","journal-title":"Aquaculture"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/1477-7827-3-69","article-title":"Induction and inhibition of oocyte maturation by EDCs in zebrafish","volume":"3","author":"Tokumoto","year":"2005","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.tiv.2018.09.017","article-title":"Disruption of oocyte maturation by selected environmental chemicals in zebrafish","volume":"54","author":"Maskey","year":"2019","journal-title":"Toxicol. In Vitro"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1095\/biolreprod.102.008086","article-title":"Evolution and nomenclature of the zona pellucida gene family","volume":"68","author":"Spargo","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4674","DOI":"10.1111\/j.1742-4658.2010.07874.x","article-title":"Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica","volume":"277","author":"Sano","year":"2010","journal-title":"FEBS J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cbpc.2018.08.008","article-title":"Expression of zona pellucida 3 gene is regulated by 17\u03b1-ethinylestradiol in adult topmouth culter Culter alburnus","volume":"214","author":"Qi","year":"2018","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/(SICI)1098-2795(199607)44:3<295::AID-MRD3>3.0.CO;2-H","article-title":"Molecular cloning, structural analysis, and expression of carp ZP3 gene","volume":"44","author":"Chang","year":"1996","journal-title":"Mol. Reprod. Dev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5640","DOI":"10.4238\/2013.November.18.13","article-title":"Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis","volume":"12","author":"Shi","year":"2013","journal-title":"Genet. Mol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s10695-017-0434-4","article-title":"Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia","volume":"44","author":"Wu","year":"2018","journal-title":"Fish. Physiol. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1080\/15287394.2014.880393","article-title":"Changes in ultrastructure and expression of steroidogenic factor-1 in ovaries of zebrafish Danio rerio exposed to glyphosate","volume":"7","author":"Armiliato","year":"2014","journal-title":"J. Toxicol. Environ. Health A"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"15147","DOI":"10.1007\/s11356-020-11527-z","article-title":"Reproductive toxicity of Roundup WG\u00ae herbicide: Impairments in ovarian follicles of model organism Danio rerio","volume":"28","author":"Davico","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"57","DOI":"10.25225\/fozo.v59.i1.a9.2010","article-title":"Alien fish species in the Czech Republic and their impact on the native fish fauna","volume":"59","author":"Lusk","year":"2010","journal-title":"Folia Zool."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3750\/AIP2011.42.2.01","article-title":"The non-indigenous freshwater fishes of Poland. Threats to the native ichthyofauna and consequences for the fishery: A review","volume":"42","author":"Witkowski","year":"2012","journal-title":"Acta Ichthyol. Et Piscat."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1002\/aqc.2580","article-title":"A review and meta-analysis of growth and life-history traits of a declining European freshwater fish, crucian carp Carassius carassius","volume":"26","author":"Tarkan","year":"2016","journal-title":"Aquat. Conserv. Mar. Freshw. Ecosyst."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1095\/biolreprod41.1.68","article-title":"Development of an enzyme-linked immunosorbent assay for goldfish gonadotropin","volume":"41","author":"Kah","year":"1989","journal-title":"Biol. Reprod."},{"key":"ref_46","unstructured":"Brzuska, E., and Bieniarz, K. (1977). Metoda Przy\u017cyciowego Okre\u015blania Dojrza\u0142o\u015bci P\u0142ciowej Samic Karpia w Zwi\u0105zku z Iniekcjami Homogenatu Przysadki M\u00f3zgowej Karpia\u2014Broszura Nr 105, Wyd, IRS."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"36","DOI":"10.17221\/2721-CJAS","article-title":"The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching","volume":"53","author":"Szczerbik","year":"2008","journal-title":"Czech J. Anim. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1007\/s10695-016-0205-7","article-title":"Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations","volume":"42","author":"Golshan","year":"2016","journal-title":"Fish. Physiol. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3389\/fendo.2020.00175","article-title":"Seasonal Related Multifactorial Control of Pituitary Gonadotropin and Growth Hormone in Female Goldfish: Influences of Neuropeptides and Thyroid Hormone","volume":"11","author":"Ma","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_51","first-page":"2877","article-title":"A rapid approach to assess estrogenic transcriptional activityof bisphenol A in the liver of goldfish","volume":"19","author":"Golshan","year":"2020","journal-title":"Iran. J. Fish. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1007\/s11356-019-04596-2","article-title":"Effects of glyphosate and a commercial formulation Roundup\u00ae exposures on maturation of Xenopus laevis oocytes","volume":"27","author":"Slaby","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e2014021","DOI":"10.5620\/eht.e2014021","article-title":"The role of chorion on toxicity of silver nanoparticles in the embryonic zebrafish assay","volume":"29","author":"Kim","year":"2014","journal-title":"Environ. Anal. Health Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1002\/etc.4899","article-title":"Perfluorooctanesulfonic Acid\u2013Induced Toxicity on Zebrafish Embryos in the Presence or Absence of the Chorion","volume":"40","author":"Mylroie","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"123389","DOI":"10.1016\/j.jhazmat.2020.123389","article-title":"Effects of the chorion on the developmental toxicity of organophosphate esters in zebrafish embryos","volume":"401","author":"Tran","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/s41598-019-57113-3","article-title":"Exploitation of drosophila choriogenesis process as a model cellular system for assessment of compound toxicity: The phloroglucinol paradigm","volume":"10","author":"Keramaris","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1007\/s10695-018-0507-z","article-title":"Endocrine-disrupting chemicals in aquatic environment: What are the risks for fish gametes?","volume":"44","author":"Carnevali","year":"2018","journal-title":"Fish Physiol. Biochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.chemosphere.2011.12.047","article-title":"Gene expression profiles in the testis associated with testis\u2013ova in adult Japanese medaka (Oryzias latipes) exposed to 17\u03b1-ethinylestradiol","volume":"87","author":"Hirakawa","year":"2012","journal-title":"Chemosphere"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.fct.2013.05.057","article-title":"Glyphosate induces human breast cancer cells growth via estrogen receptors","volume":"59","author":"Thongprakaisang","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_60","unstructured":"Socha, M., Dr\u0105g-Kozak, E., Soko\u0142owska-Miko\u0142ajczyk, M., and Chyb, J. (2019, January 13\u201315). Effect of herbicide Roundup and tamoxifen on Prussian carp (Carassius gibelio B.) oocyte maturation and secretion of 17\u03b120\u03b2-p in vitro. Proceedings of the 5th Winter Workshop of the Society for Biology of Reproduction, Zakopane, Poland. Unpublished Results."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1021\/tx015543g","article-title":"Pesticide Roundup provokes cell division dysfunction at the level of CDK1\/cyclin B activation","volume":"15","author":"Marc","year":"2002","journal-title":"Chem. Res. Toxicol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/1\/105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:52:58Z","timestamp":1760147578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/1\/105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,27]]},"references-count":61,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["ani13010105"],"URL":"https:\/\/doi.org\/10.3390\/ani13010105","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,27]]}}}