{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T14:47:50Z","timestamp":1777560470391,"version":"3.51.4"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T00:00:00Z","timestamp":1672358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572434"],"award-info":[{"award-number":["31572434"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["201903a06020013"],"award-info":[{"award-number":["201903a06020013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022YL007"],"award-info":[{"award-number":["2022YL007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Science and Technology Program of the Anhui Province","award":["31572434"],"award-info":[{"award-number":["31572434"]}]},{"name":"the Science and Technology Program of the Anhui Province","award":["201903a06020013"],"award-info":[{"award-number":["201903a06020013"]}]},{"name":"the Science and Technology Program of the Anhui Province","award":["2022YL007"],"award-info":[{"award-number":["2022YL007"]}]},{"name":"the Grant from Anhui Academy of Agricultural Sciences","award":["31572434"],"award-info":[{"award-number":["31572434"]}]},{"name":"the Grant from Anhui Academy of Agricultural Sciences","award":["201903a06020013"],"award-info":[{"award-number":["201903a06020013"]}]},{"name":"the Grant from Anhui Academy of Agricultural Sciences","award":["2022YL007"],"award-info":[{"award-number":["2022YL007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Improvements in the feeding of calves are of increasing importance for the development of the dairy industry. While colostrum is essential for the health of newborn calves, knowledge of protein phosphorylation alterations in neonatal calves that are fed colostrum or mature milk is lacking. Here, mid-jejunum tissue samples were collected from calves that received colostrum or milk. Subsequently, the jejunum phosphoproteome was analyzed using a phosphopeptide enrichment method, i.e., titanium immobilized metal ion affinity chromatography, coupled with liquid chromatography-tandem mass spectrometry. A total of 2093 phosphopeptides carrying unique 1851 phosphorylation sites corresponding to 1180 phosphoproteins were identified. Of the 1180 phosphoproteins, 314 phosphorylation sites on 241 proteins were differentially expressed between the groups. Gene ontology analysis indicated that the phosphoproteins were strongly associated with developmental and macromolecule metabolic processes, signal transduction, and responses to stimuli and insulin. Pathway analysis showed that the spliceosome, Hippo, insulin, and neurotrophin signaling pathways were enriched. These results reveal the expression pattern and changes in the function of phosphoproteins in bovine jejunum tissues under different feeding conditions and provide further insights into the crucial role of colostrum feeding during the early stages of life.<\/jats:p>","DOI":"10.3390\/ani13010145","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T03:26:11Z","timestamp":1672370771000},"page":"145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phosphoproteomic Analysis of the Jejunum Tissue Response to Colostrum and Milk Feeding in Dairy Calves during the Passive Immunity Period"],"prefix":"10.3390","volume":"13","author":[{"given":"Xiaowei","family":"Zhao","sequence":"first","affiliation":[{"name":"Anhui Key Laboratory of Animal and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxia","family":"Qi","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Animal and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Animal and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanglong","family":"Cheng","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Animal and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.3168\/jds.S0022-0302(03)73765-5","article-title":"Intestinal morphology, epithelial cell proliferation, and absorptive capacity in neonatal calves fed milk-born insulin-like growth factor-I or a colostrum extract","volume":"86","author":"Roffler","year":"2003","journal-title":"J. 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